The role of ischemic preconditioning in gene expression related to inflammation in a rat model of intestinal ischemia-reperfusion injury

Abstract

Purpose: To investigate the gene expression related to inflammation on mice subjected to intestinal ischemia and reperfusion (I/R) and treated with ischemic preconditioning (IPC). Methods: Thirty rats (EPM-Wistar), distributed in five groups of six animals each, were underwent anesthesia and laparotomy. The ischemia time was standardized in 60 minutes and the reperfusion time 120 minutes. IPC was standardized in 5 minutes of ischemia followed by 10 minutes of reperfusion accomplished before I/R. The control group was submitted only to anesthesia and laparotomy. The other groups were submitted to ischemia, I/R, ischemia + IPC and I/R + IPC. It was collected a small intestine sample to analyses by Quantitative Polymerase Chain Reaction in real Time (RT-qPCR) and histological analyses. It was studied 27 genes. Results: The groups that received IPC presented downregulation of genes, observed in of genes in IPC+ischemia group and IPC+I/R group. Data analysis by clusters showed upregulation in I/R group, however in IPC groups occurred downregulation of genes related to inflammation. Conclusion: The ischemia/reperfusion promoted upregulation of genes related to inflammation, while ischemic preconditioning promoted downregulation of these genes.

Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

“…The effectiveness of antioxidant treatments on IRI has been demonstrated by a large number of previous studies [19][20][21][22][23][24]. Our results are in accordance with this and show that nitroindazole reduces intestinal mucosal damage after i-IRI.…”

Edaravone reduces oxidative stress and intestinal cell apoptosis after burn through up-regulating miR-320 expression

“…Severe burn injury, increases intestinal microvascular permeability increases, damages intestinal cell necrosis, apoptosis and intestinal mucosal barrier function impair, lead to displacement of intestinal bacterial and toxin, induction of systemic inflammatory response syndrome and multiple organ dysfunction syndromes (Zhang et al, 2017a; Osuka et al, 2017) . Recent studies showed that hypoxic ischemia and reperfusion injury in intestinal tissue after burn injury is possibly a main contributing to intestinal barrier damage (Miranda et al, 2018; Zhang et al, 2017b; Zhou et al, 2015; Tassopoulos et al, 2017). Therefore, protecting the function of intestinal mucosal barrier after burn injury could be effective to the prevention and treatment of intestinal infection and multiple organ dysfunctions.…”

The role of ischemic preconditioning in the expression of apoptosis-related genes in a rat model of intestinal ischemia-reperfusion injury

“…Ischemia was confirmed by observing the pale appearance of the clamped intestine and the lack of arterial beating. All animal procedures were carried out as previously described 7 .…”

“…Then, 2 μg of RNA was used for reverse transcription into cDNA and Real-time PCR was run in a thermal cycler (model MX3000P, Applied Biosystems). The assays were performed in 96-well plates to detect the expression of 84 genes related to Endothelial Cell Biology, which contain 28 genes related to apoptosis (PARN-015Z, QIAGEN) 7 . The conditions for PCR were 95°C for 5 min, 40 amplification cycles of 95°C for 30 s, 58°C for 30 s, and extension at 72°C for 45 s. β-actin served as an internal reference and the expression of target genes was normalized to that of β-actin.…”

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