Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion

Castro‐e‐Silva, Orlando; D’Albuquerque, Luiz Augusto Carneiro; Silveira, Marina Rodrigues Garcia da; Zorzi, Patrícia; Liu, Jordan Bistafa; Campos, Daniel Tófoli Queiroz; Victorino, João Paulo; Jordani, Maria Cecília; Mendes, Karina Dal Sasso; Évora, Paulo Roberto Barbosa

doi:10.1590/s0102-865020180120000001

Cited by 2 publications

(2 citation statements)

References 27 publications

(33 reference statements)

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“…However, such consumption rates, when expressed in reason -RCR -are attenuated, what would probably be corrected with an increase in the number of animals in each group. The results found in the three parameters are similar to those obtained by Castro e Silva 35 in the treatment with Methylene Blue, which supports the pharmacological similarity of the compounds considered in this study 19 .…”

Section: ■ Discussionsupporting

confidence: 90%

Evaluation of Indigo carmine on hepatic ischemia and reperfusion injury

et al. 2020

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To evaluate the effects of treatment with Indigo Carmine (IC) on rat livers subjected to ischemia-reperfusion injury. Methods: The animals were subdivided into 4 groups: 1.SHAM group(SH)-saline; 2.SHAM group with IC-2mg/Kg(SHIC); 3.IR group-rats submitted to ischemia and reperfusion with saline(IR); 4.IR group with IC-2mg/Kg(IRIC). The IR protocol consists of liver exposure and administration of drug or saline intravenously, followed by 60 minutes of ischemia and 15 of reperfusion. Liver samples were collected for biochemical analysis. Results: State 3 of mitochondrial respiration showed a significant worsening of the IRIC group in relation to all others. State 4 showed a difference between IRIC and SHIC. The Respiratory Control Ratio showed statistical decrease in IR and IRIC versus Sham. The osmotic swelling showed significant difference between SHxIR; SHICxIRIC and SHxIRIC. There was a significant increase in ALT in the IRIC group in relation to all the others. Concerning the nitrate dosage, there was a decrease in the group treated with IC(IRxIRIC). There was no difference regarding the dosage of Malondialdehyde. Conclusion: IC was not able to protect mitochondria from IR injury and proved to be a potentiating agent, acting in synergy with the IR injury promoting damage to the hepatocyte membranes.

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Section: ■ Discussionsupporting

confidence: 90%

Evaluation of Indigo carmine on hepatic ischemia and reperfusion injury

et al. 2020

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“…NO/cGMP inhibition would make a difference in the treatments of vasoplegia and IRI. Our 25 years of experimental and clinical experience have led us to believe that methylene blue (MB) is a compound that can be effectively employed [18,49,296,297,298,299,300,301]. The effects of MB are apparent only regarding NO up-regulation, while the compound, per se, cannot be considered to act as a vasoconstrictor.…”

Section: Therapeutics: the Pharmacological Approachmentioning

confidence: 99%

Ischemia/Reperfusion Injury Revisited: An Overview of the Latest Pharmacological Strategies

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Jordani

et al. 2019

IJMS

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Ischemia/reperfusion injury (IRI) permeates a variety of diseases and is a ubiquitous concern in every transplantation proceeding, from whole organs to modest grafts. Given its significance, efforts to evade the damaging effects of both ischemia and reperfusion are abundant in the literature and they consist of several strategies, such as applying pre-ischemic conditioning protocols, improving protection from preservation solutions, thus providing extended cold ischemia time and so on. In this review, we describe many of the latest pharmacological approaches that have been proven effective against IRI, while also revisiting well-established concepts and presenting recent pathophysiological findings in this ever-expanding field. A plethora of promising protocols has emerged in the last few years. They have been showing exciting results regarding protection against IRI by employing drugs that engage several strategies, such as modulating cell-surviving pathways, evading oxidative damage, physically protecting cell membrane integrity, and enhancing cell energetics.

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Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion

Cited by 2 publications

References 27 publications

Evaluation of Indigo carmine on hepatic ischemia and reperfusion injury

Evaluation of Indigo carmine on hepatic ischemia and reperfusion injury

Ischemia/Reperfusion Injury Revisited: An Overview of the Latest Pharmacological Strategies

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