Analysis of BMSCs-intervened viscoelasticity of sciatic nerve in rats with chronic alcoholic intoxication

Abstract

Purpose: To investigate the impact of bone mesenchymal stem cells (BMSCs) intervention on the viscoelasticity of sciatic nerve in rats with chronic alcohol intoxication (CAI). Methods: The CAI rat models were prepared, divided into model groups, and treated with either BMSCs or basic fibroblast growth factor (bFGF). Then the rats underwent electrophysiological test and the serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), and metallothionein (MT) were measured. Histological observation, stress relaxation test, and creep test were performed for the sciatic nerve of the CAI model in each group. Results: The MDA level of group BMSC was significantly lower (p<0.05) than that of groups MOD (the CIA model) and bFGF. The SOD and MT levels were higher in group BMSC than in groups MOD and bFGF (p<0.05). The motor nerve conduction velocity and amplitude were higher in group BMSC than in groups MOD and bFGF (p<0.05). The amounts of 7200s stress reduction and 7200 s strain increase of the sciatic nerve in group BMSC were greater than those in groups bFGF and MOD (p<0.05). Conclusion: Bone mesenchymal stem cells can improve the metabolism of free radicals, restore the tissue morphology and viscoelasticity of the chronic alcohol intoxication animal model, and positively affect the repairing of the injured sciatic nerve.

Hybrid Bionic Nerve Interface for Application in Bionic Limbs

“…In the previous study, the NCV of 50 to 70 m −1 s and the muscle conduction velocity (MCV) of ≈35 m −1 s were reported in normal rabbits. [ 37–39 ] These discrepancies are likely due to the incomplete recovery from the nerve damage and transection, which generally causes the reduction of NCV by ≈50%. [ 40–44 ]…”

“…In the previous study, the NCV of 50 to 70 m −1 s and the muscle conduction velocity (MCV) of ≈35 m −1 s were reported in normal rabbits. [37][38][39] These discrepancies are likely due to the incomplete recovery from the nerve damage and transection, which generally causes the reduction of NCV by ≈50%. [40][41][42][43][44] Figure 3f shows the signal amplitudes recorded at the BI-R depending on the stimulation intensity, indicating that the amplitude of the recorded signals increased with increasing stimulation intensity.…”

Comparative Analysis of Bone Mechanical Properties of Adipose-Derived Mesenchymal Stem Cells and Raloxifene in Treatment of Osteoporosis

“…One MODEL-55100 electronic universal testing machine produced by Changchun testing Changchun Testing Machine Research Institute (Changchun, Jilin, China) was used for the test according to the reference 11,12) after sample pre-conditioning. 12 femoral samples were randomly selected from group CON and other experimental groups, respectively, placed on the test machine table, and applied the load to the sample at a speed of 2 mm/min until the samples were destroyed.…”

Mechanisms and Treatments of Peripheral Nerve Injury

“…3 Many factors are involved in the repair of peripheral nerve damage, for instances, the Schwann cells, fibroblasts, extracellular matrix (ECM), damaged nerve distal stump, inflammatory cytokines, and neurotrophic factors and so on. The current treatment of PNJ mainly includes microsurgery, 4 autologous nerve transplantation, 5 allogeneic nerve transplantation, 6 and tissue engineering technology. 7 The main bottleneck of the current treatments of PNJ is the shortage of donor nerves.…”