Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Schiavoni, Vagner Sarraipo; Silva, Jairo Pinheiro da; Neto, Fermino Sanches Lizarte; Assis, Múcio Luiz Cirino de; Tazima, Maria de Fátima Galli Sorita; Carvalho, C.; Tirapelli, Daniela Pretti da Cunha; Carlotti, Carlos Gilberto; Colli, Benedicto Oscar; Tirapelli, Luís Fernando

doi:10.1590/s0102-865020180080000001

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…Among the findings of recent studies of our group, neuronal loss was also higher in the medial striatum region of the animals of groups I and I + A and the protein expression of CASPASE -3 was higher than that of XIAP in groups I and I + A for both proteins. (Carvalho et al, 2016;Schiavoni et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Research Article Morphological, immunohistochemical and miRNA21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model

Tirapelli¹,

Cirino²,

Silva³

et al. 2020

Genet. Mol. Res.

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Cerebral ischemia is one of the main causes of morbidity and mortality as Abusive use of alcohol also causes health problems, along with considerable social and economic repercussions. We examined how these two conditions affect histopathological signs, morphometrics, expression of apoptosisrelated proteins CASPASE 3 and BCL2, and serum gene expression of miRNA-21, in cerebral ischemia associated with a chronic alcoholism model in brain tissue (striatum, lateral and dorsolteral cortex). Fifty adult Wistar rats were divided into five groups: control (C), sham (S), ischemic (I), alcoholic (A) and ischemic/alcoholic ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 19 (4): gmr18624 D.C.P. Tirapelli et al. 2 groups (I+A). Brain samples were processed for histopathological, morphological, and immunohistochemical analyses for the expression of CASPASE 3 and BCL2, and blood was collected for analysis of miRNA-21 gene expression by real-time PCR. Histopathological changes such as neurons with pyknotic nuclei and diffuse neurons with loss of the contour of their cellular membranes and cytoplasmic edema, were observed in groups I and I+A in all three areas. Protein expression of CASPASE 3 was significantly higher than that of BCL2, with higher expression in groups I and I+A for these two proteins. Serum expression of miRNA-21 was low in all groups, and was slightly higher in groups I and I+A, with no significant differences. The histopathological and morphometric alterations, which were observed mainly in the ischemic animals, correlated with the expression of CASPASE 3. The expression of BCL2 was greater where histopathological changes and expression of CASPASE 3 were less evident. The I and I+A groups presented more histopathological changes, with greater neuronal loss and cerebral edema, and greater expression of CASPASE 3, suggesting that ischemia and alcoholism, when associated, can cause considerable injury and damage to brain tissue.

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Section: Discussionmentioning

confidence: 99%

Research Article Morphological, immunohistochemical and miRNA21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model

Tirapelli¹,

Cirino²,

Silva³

et al. 2020

Genet. Mol. Res.

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Antidotes Against Methanol Poisoning: A Review

Pohanka

2019

MRMC

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Methanol is the simplest alcohol. Compared to ethanol that is fully detoxified by metabolism. Methanol gets activated in toxic products by the enzymes, alcohol dehydrogenase and aldehyde dehydrogenase. Paradoxically, the same enzymes convert ethanol to harmless acetic acid. This review is focused on a discussion and overview of the literature devoted to methanol toxicology and antidotal therapy. Regarding the antidotal therapy, three main approaches are presented in the text: 1) ethanol as a competitive inhibitor in alcohol dehydrogenase; 2) use of drugs like fomepizole inhibiting alcohol dehydrogenase; 3) tetrahydrofolic acid and its analogues reacting with the formate as a final product of methanol metabolism. All the types of antidotal therapies are described and how they protect from toxic sequelae of methanol is explained.

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Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Abstract: The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.

Cited by 2 publications

References 26 publications

Research Article Morphological, immunohistochemical and miRNA21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model

Research Article Morphological, immunohistochemical and miRNA21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model

Antidotes Against Methanol Poisoning: A Review

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