Impact of 1,25(OH) 2 D 3 on TG content in liver of rats with type 2 diabetes

Abstract

Purpose: To evaluate the effects of 1,25 dihydroxy vitamin D3 (1,25(OH) 2 D 3 ) on the content of triglyceride (TG), as well as on the gene and protein expressions of adiponectin receptor 2 (AdipoR2), p38 mitogen-activated protein kinase (P38MAPK), and lipoprotein lipase (LPL) in the liver of rats with type 2 diabetes mellitus (T2DM) so as to provide theoretical basis for exploring the mechanism by which 1,25(OH) 2 D 3 regulates TG. Methods: Wistar rats were divided into four groups (n=25), with different treatments and detected the gene and protein expressions of AdipoR2, p38MAPK, and LPL in the liver tissue by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Meanwhile, the content of TG in the liver tissue was detected by the Enzyme-linked immunosorbent assay. Results: The expression of AdipoR2, p38MAPK, LPL gene and protein in the liver of VitD intervention group was significantly higher than that in T2DM group (P <0.05), while the TG content was significantly lower than that in T2DM group (P <0.05). Conclusion: 1,25(OH) 2 D 3 can decrease the content of TG in the liver, and its mechanism may be achieved by upregulating the expressions of AdipoR2, p38MAPK, and LPL in the liver.

Low Vitamin D is associated with Coronary Atherosclerosis in Women with HIV

“…Until recently, the mechanism by which vitamin D exerts its effects on cholesterol metabolism was unclear. Using a rat model of type 2 diabetes mellitus, Yang et al [21] demonstrated that vitamin D increased expression of adiponectin receptor 2 (adi-poR2), p28 mitogen-activated protein kinase (MAPK) and lipoprotein lipase (LPL), which are involved in fatty acid oxidation and triglyceride catabolism in chylomicrons and very low density lipoprotein (VLDL). Furthermore, vitamin D may play an intricate role in the development of non-traditional risk factors for CVD.…”

1α,25(OH)2D3 alleviates high glucose–induced lipid accumulation in rat renal tubular epithelial cells by inhibiting SREBPs

“…7 A number of population-based studies support the connection between vitamin D and lipid accumulation. 8,9 Some studies indicate that vitamin D can prevent lipid accumulation, [10][11][12][13][14] while others believe the opposite, 15,16 suggesting that the central regulation mechanisms of vitamin D involved in the initiation and accumulation of lipids are still remaining controversial.…”