Effects of the application of the amniotic membrane in the healing process of skin wounds in rats

Campelo, Mariana Barbosa Dias; Santos, Joelita de Alencar Fonseca; Filho, Antônio Luíz Martins Maia; Ferreira, Daniel Cabral Leão; Sant’Anna, Luciana Barros; Oliveira, Rauirys Alencar de; Maia, Leonardo Fonseca; Arisawa, Emília Ângela Lo Schiavo

doi:10.1590/s0102-865020180020000006

Cited by 17 publications

(9 citation statements)

References 24 publications

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“…Soluble factors released by the Am cells and present in their stroma play an important role in the treatment of inflammatory and fibrotic diseases, with their medicinal capacity being dependent on time, dosage and location of the cytokine / growth factor produced by the amnion, together with the influence of the receptor tissue microenvironment (Silini et al 2013). Considering that, Am acts mainly as a matrix and as a source of bioactive factors that diffuse through the tissue (Manuelpillai et al 2011, Silini et al 2013, we apply the membrane with the mesenchymal side facing the hepatic tissue, corroborating the previous studies of our laboratory which reported better results when this protocol was followed (Sant'Anna et al 2011a, Nicodemo et al 2017, Campelo et al 2018.…”

Section: Discussionsupporting

confidence: 83%

Antifibrotic effects of total or partial application of amniotic membrane in hepatic fibrosis

Mamede

Sant’Anna

2019

An. Acad. Bras. Ciênc.

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Liver fibrosis is the final common pathway of chronic liver diseases, having cirrhosis as a possible progression, which has liver transplantation as the only effective treatment. Human amniotic membrane represents a potential strategy as a therapy for liver fibrosis, due to its anti-inflammatory, antifibrotic and immunomodulatory properties. The aim of this study was to evaluate amniotic membrane effects as a treatment for hepatic fibrosis induced in rats by bile duct ligation (BDL), verifying alterations between two different forms of amniotic membrane application, around all the lobes of the liver and around only one lobe of the liver. Two weeks after inducing fibrosis, an amniotic membrane fragment was applied to the surface of the liver, covering it either totally or partially. Four weeks later, the animals were euthanized and liver samples were collected. Histopathological and quantitative analyses demonstrated fibrosis severity decrease and an extremely significant reduction in the deposition of collagen in the groups treated with amniotic membrane, particularly when the amniotic membrane was applied in only one liver lobe. It is concluded that the amniotic membrane acted on the repair of liver fibrosis in both modes of application, with the application of the amniotic membrane around only one hepatic lobe being more effective in reducing the severity / extent of fibrosis.

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Section: Discussionsupporting

confidence: 83%

Antifibrotic effects of total or partial application of amniotic membrane in hepatic fibrosis

Mamede

Sant’Anna

2019

An. Acad. Bras. Ciênc.

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“…The dermis of rodent skin is mainly composed of types I and III collagen and elastic fibers [61]. It is well known that collagen fibers of normal skin are different from that of scar tissue.…”

Section: Discussionmentioning

confidence: 99%

Hypoxic preconditioning of human urine-derived stem cell-laden small intestinal submucosa enhances wound healing potential

et al. 2020

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Background Urine-derived stem cells (USCs) are a valuable stem cell source for tissue engineering because they can be harvested non-invasively. Small intestine submucosa (SIS) has been used as scaffolds for soft tissue repair in the clinic. However, the feasibility and efficacy of a combination of USCs and SIS for skin wound healing has not been reported. In this study, we created a tissue-engineered skin graft, termed the SIS+USC composite, and hypothesized that hypoxic preconditioning would improve its wound healing potential. Methods USCs were seeded on SIS membranes to fabricate the SIS+USC composites, which were then cultured in normoxia (21% O2) or preconditioned in hypoxia (1% O2) for 24 h, respectively. The viability and morphology of USCs, the expression of genes related to wound angiogenesis and reepithelialization, and the secretion of growth factors were determined in vitro. The wound healing ability of the SIS+USC composites was evaluated in a mouse full-thickness skin wound model. Results USCs showed good cell viability and morphology in both normoxia and hypoxic preconditioning groups. In vitro, hypoxic preconditioning enhanced not only the expression of genes related to wound angiogenesis (VEGF and Ang-2) and reepithelialization (bFGF and EGF) but also the secretion of growth factors (VEGF, EGF, and bFGF). In vivo, hypoxic preconditioning significantly improved the wound healing potential of the SIS+USC composites. It enhanced wound angiogenesis at the early stage of wound healing, promoted reepithelialization, and improved the deposition and remodeling of collagen fibers at the late stage of wound healing. Conclusions Taken together, this study shows that hypoxic preconditioning provides an easy and efficient strategy to enhance the wound healing potential of the SIS+USC composite.

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“…The amnion was isolated from the chorion, and the decellularization process was initiated by snap freezing, employing liquid nitrogen for 1 min to detach the monolayer of amnions' epithelium. Confirmation was performed through H&E staining of the samples and histological observation by light microscopy 21 .…”

Section: Methodsmentioning

confidence: 99%

Synergistic efficacy of colistin and silver nanoparticles impregnated human amniotic membrane in a burn wound infected rat model

Wali

Shabbir

Wajid

et al. 2022

Sci Rep

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Antimicrobials used to treat burn wound infections have become multidrug-resistant, thus delaying wound healing. When combined with silver nanoparticles, antibiotics create a multifaceted antibacterial mechanism of action to which bacteria are incapable of developing resistance. Similarly, the amniotic membrane has been found to lower the bacterial number. The purpose of the current study was to observe the antibacterial activity of combined topical colistin with silver nanoparticles and decellularized human amniotic membrane as a dressing in burn wounds infected with bacteria with the goal of promoting faster healing. Bacteria commonly isolated from burn wounds and the most sensitive topical antibiotic were identified. Colistin, silver nanoparticles and combined colistin with silver nanoparticles were impregnated into decellularized human amniotic membranes. These wound dressings were evaluated in third-degree multidrug-resistant bacterial infected thermal burns induced in rats. Out of a total of 708 pus samples from burn wounds, Pseudomonas aeruginosa was the most prevalent pathogen 308 (43.5%), followed by Klebsiella pneumoniae 300 (42.4%). Topical colistin was 100% sensitive for both bacteria. Overall, maximum wound contraction (p < 0.05), and increased collagen deposition (+++) with no isolation of bacteria from wound swabs were noted on day 21 for the combined colistin with silver nanoparticle-loaded human amniotic membrane dressing group. Our study concluded that the increased antimicrobial activity of the novel combination of colistin and silver nanoparticle-loaded decellularized human amniotic membrane manifested its potential as an effective burn wound dressing.

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Effects of the application of the amniotic membrane in the healing process of skin wounds in rats

Cited by 17 publications

References 24 publications

Antifibrotic effects of total or partial application of amniotic membrane in hepatic fibrosis

Antifibrotic effects of total or partial application of amniotic membrane in hepatic fibrosis

Hypoxic preconditioning of human urine-derived stem cell-laden small intestinal submucosa enhances wound healing potential

Synergistic efficacy of colistin and silver nanoparticles impregnated human amniotic membrane in a burn wound infected rat model

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