Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears

Zhang, Chong; Liu, Yujie

doi:10.1590/s0102-865020170120000006

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…These roles might be affected by the concentration of IL-1β and our study showed IL-1β above 5.19 pg/ml had positive effects. TGF-β1 inhibits MMP-9 and MMP-13 expression to increase collagen accumulation [44] and is known to improve tendon strength and tendon healing [45, 46]. Our study demonstrated that TGF-β1 above 61.79 μg/ml was related to functional improvement in RC tendinopathy.…”

Section: Discussionmentioning

confidence: 53%

Effect of platelet-rich plasma on the degenerative rotator cuff tendinopathy according to the compositions

Yeo¹,

Noh²,

Ha³

et al. 2019

J Orthop Surg Res

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BackgroundThere are controversies about platelet-rich plasma (PRP) as an established treatment option for rotator cuff (RC) tendinopathy. The purpose of the study was to find the relation of cellular component with clinical efficacy in RC tendinopathy and to find the composition of PRP in treating RC tendinopathy.MethodsA total 30 patients were recruited and divided into PRP and control groups. In the PRP group, 2 ml of PRP solution was injected to the hypoechoic lesion of degenerative supraspinatus via 22-gauge syringe with peppering technique. Patients in the control group were taught rotator cuff strengthening exercises. American Shoulder and Elbow Surgeons (ASES), Constant-Murley score, and numeric rating scale (NRS) were measured before, 6 weeks after, 12 weeks after, and 24 weeks after the procedure. PRP compositions were analyzed using the 1 ml of PRP solution.ResultsLinear regression analysis showed no significant difference of ASES and Constant-Murley scores between the groups at 6 weeks (P = 0.582 and 0.258) and at 12 weeks (P = 0.969 and 0.795) but showed a significant difference at 24 weeks (P = 0.050 and 0.048). Independent t test showed significant group difference of NRS at 6 weeks (P = 0.031) but not at 12 and 24 weeks (P = 0.147 and 0.935). 5.19 pg/ml in IL-1β and 61.79 μg/ml in TGF-β1 were acquired as cutoff values to predict meaningful improvement. The PRP subgroup above IL-1β or TGF-β1 cutoff value showed significant differences in all clinical outcomes compared with the exercise group while the PRP subgroup below the cutoff value showed no significant differences in linear regression analysis.ConclusionsOur study can help to find the optimal PRP condition and to enhance the effect of PRP on RC tendinopathy.Trial registrationAll the patients were registered in our Institutional Ethics Committee (approval number 2014-05-009).

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Section: Discussionmentioning

confidence: 53%

Effect of platelet-rich plasma on the degenerative rotator cuff tendinopathy according to the compositions

Yeo¹,

Noh²,

Ha³

et al. 2019

J Orthop Surg Res

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“…TGF-β1 induces differentiation of progenitor cells in tendon, increases collagen III expression and regulates collagen VI, which is important in fibrillogenesis, thereby accelerating tendon reparation processes. These accelerated reparation processes also are connected with higher VEGF expression (8,21,28,30). Some influence on the structure of tendon depends on the biomechanical strength so, knee joint has very interesting signalling pathway during motion -when there are movements in knee joint, then articulatory cartilage squeezes and interstitial fluid from cartilage gets into articulatory cavity.…”

Section: Resultsmentioning

confidence: 99%

Characterisation of Morphology in Musculus Semitendinosus Tendon Used for Anterior Cruciate Ligament Reconstruction: A Pilot Study

Osipovs

Pilmane

Ciems

2020

Acta Chirurgica Latviensis

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SummaryIntroductionAnterior cruciate ligament (ACL) rupture is very common in athletes. In the general population, incidence is approximately 33 per 100 000 people.Aim of the studyThe aim of the study was the evaluation of morphological changes in the musculus semitendinosus tendon used for the reconstruction of ACL to understand the quality of most common for surgery used material.Material and methodsThe materials were obtained from four ACL autologous hamstring reconstruction surgeries. The tissue was stained with hematoxyllin and eosin and with immunohistochemical (IMH) staining of PGP9.5, VEGF, collagen I and collagen III. The material was evaluated with semiquantitative method.ResultsRoutine staining showed practically unchanged tendon structure, with one exception when sclerotic blood vessels were observed in endotenon. Collagen III IMH demonstrated moderate to numerous positive collagen fibres in two cases, but in other two there were only few positive structures seen. Collagen I IMH showed few to moderate number of positive collagen fibres in all cases. In two cases, moderate number of PGP9.5 positive nerve fibres was observed and in two other cases occasional to few number of positive structures was detected. PGP 9.5 marked higher number of nerve fibres in peritenon than in endotenon. Numerous VEGF positive endotheliocytes were observed in two cases, but in two other cases VEGF positive endotheliocytes were occasional.ConclusionsTendon of musculus semitendinosus displays two patterns of distribution of tissue ischemia, neuropeptide containing innervation and collagen I and III. Collagen III is thought to be evaluated as a response of tendon to the ischemia and intensive innervation, while increase of collagen I probably is related to the relatively unchanged vascularity and innervation. The pattern of musculus semitendinosus tendon structural changes seems to be connected to the individual homeostasis in patients persisting before the usage of tendon for the reconstruction.

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“…17 TGF-b1 inhibits matrix metalloproteinase-9 and -13 expression and increases collagen accumulation, improving tendon strength and tendon healing. 1,39 Based on these data, it is possible that higher levels of leukocytes and TGF-b1 in LR-PRP might have better efficacy in RC repair compared with LP-PRP. type III collagen (COL3), scleraxis (SCX), and decorin (DCN) were higher in tenocytes in the PRP groups than in the control group, with LR-PRP higher than LP-PRP.…”

Section: Discussionmentioning

confidence: 99%

“… 17 TGF-β1 inhibits matrix metalloproteinase–9 and -13 expression and increases collagen accumulation, improving tendon strength and tendon healing. 1 , 39 Based on these data, it is possible that higher levels of leukocytes and TGF-β1 in LR-PRP might have better efficacy in RC repair compared with LP-PRP.…”

Section: Discussionmentioning

confidence: 99%

Leukocyte-Rich Platelet-Rich Plasma Has Better Stimulating Effects on Tenocyte Proliferation Compared With Leukocyte-Poor Platelet-Rich Plasma

Lin

Chen

et al. 2022

Orthopaedic Journal of Sports Medicine

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Background: Rotator cuff (RC) tendinopathy is one of the most common causes of shoulder pain. Platelet-rich plasma (PRP) has been frequently used in clinical scenarios, but its efficacy remains inconsistent. Purpose: To investigate the different responses of human tenocytes from torn RCs to leukocyte-rich PRP (LR-PRP) and leukocyte-poor PRP (LP-PRP) in a 2-chamber coculture device. Study Design: Controlled laboratory study. Methods: PRP was prepared using different platelet and leukocyte concentrations according to 5 groups: (1) LR-PRP with 5000 platelets/µL, (2) LR-PRP with 10,000 platelets/µL, (3) LP-PRP with 5000 platelets/µL, (4) LP-PRP with 10,000 platelets/µL, and (5) control with only culture medium supplementation and without PRP stimulation. Platelet-derived growth factor–AB (PDGF-AB) and transforming growth factor–β1 (TGF-β1) were measured in LR-PRP and LP-PRP via enzyme-linked immunosorbent assay. Microscopy, water-soluble tetrazolium salt assay, and quantitative real-time polymerase chain reaction were used to investigate the morphology, proliferation, and gene expression of RC tenocytes exposed to different PRP formulations. Data were collected from at least 3 independent measurements. The results were analyzed via 1-way analysis of variance, followed by the post hoc Bonferroni test. Results: The ratio of leukocytes to 5000 platelets/µL was 29.5 times higher in LR-PRP than in LP-PRP ( P < .05). In the 5000 platelets/µL groups, the levels of TGF-β1 and PDGF-AB were both significantly higher in LR-PRP versus LP-PRP (TGF-β1: 367.0 ± 16.5 vs 308.6 ± 30.3 pg/mL, respectively [ P = .043]; PDGF-AB: 172.1 ± 1.8 vs 94.1 ± 4.2 pg/mL, respectively [ P < .001]). Compared with the control group, RC tenocyte proliferation was 1.42 ± 0.01 and 1.41 ± 0.03 times higher in the LR-PRP groups with 5000 platelets/µL and 10,000 platelets/µL, respectively ( P < .05). The expression of tenocyte-related genes was higher in tenocytes cultured in LR-PRP. Conclusion: Both the LR-PRP groups with 5000 platelets/µL and 10,000 platelets/µL induced more growth factor release and increased RC tenocyte proliferation than did the LP-PRP groups. Clinical Relevance: In RC repair, LR-PRP may be better than LP-PRP for increasing the proliferation of tenocytes.

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Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor β1 (TGF-β1) in rotator cuff tears

Cited by 11 publications

References 33 publications

Effect of platelet-rich plasma on the degenerative rotator cuff tendinopathy according to the compositions

Effect of platelet-rich plasma on the degenerative rotator cuff tendinopathy according to the compositions

Characterisation of Morphology in Musculus Semitendinosus Tendon Used for Anterior Cruciate Ligament Reconstruction: A Pilot Study

Leukocyte-Rich Platelet-Rich Plasma Has Better Stimulating Effects on Tenocyte Proliferation Compared With Leukocyte-Poor Platelet-Rich Plasma

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