Alternative animal model for studies of total skin thickness burns

Abstract

Purpose: To present an alternative experimental model of third degree burn of easy reproducibility. Methods: Eighteen male Wister rats were randomly divided into three groups, 6 of which were allocated to each group. A soldering iron coupled to an aluminum plate was used to produce burn, at a temperature of 150ºC, with different exposure times per group. Group 5 (G5) animals were burned at 150°C with exposure time of 5 seconds; Group 10 (G10) the animals were burned at 150°C with exposure time of 10 seconds and group 15 (G15) the animals were burned at 150°C with exposure time of 15 seconds. Results: Histopathological analyzes showed that all three groups had similar morphological characteristics, with total thickness involvement. Conclusion:The technique is effective to reproduce a third degree burn and suggests the temperature of 150ºC with 5 seconds of exposure in order to minimize the risks to the animals.

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

“…The animals were anesthetized with ketamine (95 mg/kg) and Xylazine (12 mg/kg) intraperitoneally and later trichotomized by the digital technique in the dorsal region corresponding to an area of 4 × 5 cm. Afterwards, the burn process was started on the skin of the animal through a circular 3‐cm diameter aluminum plate coupled to a soldering iron with a temperature of 150°C (de Andrade et al, 2017; Venter, Monte‐Alto‐Costa, & Marques, 2015). The plate was positioned on the back and maintained for 5 s, these parameters being already pre‐established in the literature (de Andrade et al, 2017), totaling a burned area of 7.1 cm 2 that corresponds to approximately 2% of the total body surface area (TBSA).…”

“…Afterwards, the burn process was started on the skin of the animal through a circular 3‐cm diameter aluminum plate coupled to a soldering iron with a temperature of 150°C (de Andrade et al, 2017; Venter, Monte‐Alto‐Costa, & Marques, 2015). The plate was positioned on the back and maintained for 5 s, these parameters being already pre‐established in the literature (de Andrade et al, 2017), totaling a burned area of 7.1 cm 2 that corresponds to approximately 2% of the total body surface area (TBSA). The TBSA was calculated with Meeh's (de Mesquita et al, 2010; Gouma et al, 2012) formula: TBSA (cm 2 ) = 9.1 BW2/3, where 9.1 is a rat constant and BW is body weight in grams.…”

“…The burn is defined by the International Society for Burn Injury as a traumatic injury of mostly thermal origin that affects the organic tissues. Burn wounds can be classified according to the extent reached and the impaired depth of skin (de Andrade, Parisi, Brassolatti, & Parizotto, 2017; Guirro & Guirro, 2004). The most complex burns, called total thickness (third degree) affect the three layers of the skin (epidermis, dermis, and hypodermis) and in some cases are understood as subcutaneous tissue, muscle, and bone (Jabeen et al, 2019; Venter, Marques, Santos, & Monte‐Alto‐Costa, 2016).…”