DNA damage in Wistar Kyoto rats exercised during pregnancy

Abstract

Purpose: To evaluate DNA damage levels in pregnant rats undergoing a treadmill exercise program. Methods: Wistar Kyoto rats were allocated into two groups (n= 5 animals/group): non-exercise and exercise. The pregnant rats were underwent an exercise protocol on a treadmill throughout pregnancy. Exercise intensity was set at 50% of maximal capacity during maximal exercise testing performed before mating. Body weight, blood pressure and glucose levels, and triglyceride concentration were measured during pregnancy. At day 10 post-natal, the animals were euthanized and maternal blood samples were collected for DNA damage. Results: Blood pressure and glucose levels and biochemical measurements showed no significant differences. Increased DNA damage levels were found in exercise group compared to those of non-exercise group (p<0.05). Conclusion:The exercise intensity protocol used in the study might have been exhaustive leading to maternal increased DNA damage levels, demonstrating the relevance of an adequate protocol of physical exercise.

Deneysel Egzersiz Uygulamasının Yaşlılık Sürecinde Etkileri

“…Görsel skor sistemine göre 5 farklı DNA hasarı sınıfı göz önünde bulundurularak 0-4 ölçeğinde incelenmiştir (0 = hasar yok, 4 = maksimum hasar, resim1). Bahsedilen tüm işlemler, çevre kaynaklı DNA hasarını önlemek amacıyla karanlık ortamda yapıldı [12].…”