Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage

Abstract

Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage 1Acta Cir. Bras. 2017;32(4):270-279 Abstract Purpose: To evaluate the effects of HBO (Hyperbaric oxygen) and NGF (Nerve growth factor) on the long-term neural behavior of neonatal rats with HIBD (Neonatal hypoxic ischemic brain damage). Methods:The HIBD model was produced by ligating the right common carotid artery of 7 days old SD (Sprague-Dawley) rats followed by 8% O2 + 92% N2 for 2h. Totally 40 rats were randomly divided into 5 groups including sham-operated group, HIBD control group, HBO treated group, NGF treated group and NGF + HBO treated group. The learning and memory ability of these rats was evaluated by Morris water maze at 30 days after birth, and sensory motor function was assessed by experiments of foot error and limb placement at 42 days after birth. Results:The escape latency of HBO treated group, NGF treated group and NGF + HBO treated group was shorter than that of HIBD control group (p<0.01) and longer than that of shamoperated group. The piercing indexes of 3 treated groups were higher than that of HIBD control group (p<0.01). Conclusion: Hyperbaric oxygen and nerve growth factor treatments may improve learning and memory ability and sensory motor function in neonatal rats after hypoxic ischemic brain damage. Key words: Hyperbaric Oxygenation. Nerve Growth Factor. Hypoxia-Ischemia, Brain. Rats. 2-Experimental Surgery 270Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage Wei L et. al. Acta Cir Bras. 2017;32(4):270-279 271 researchers have been performed about the roles of HBO and NGF in several aspects, but the effects of HBO and NGF on long-term neural behavior of neonatal rats have not been clearly explained.In the present study, we used SD (Sprague-Dawley) newborn rats to construct HIBD models. Furthermore, the experiments of place navigation, space search, feet errors and limb placement were made to explore learning and memory ability, and sensory motor function in neonatal rats after HIBD. We aimed to study the effects of HBO and NGF on the long-term neural behavior of neonatal rats with HIBD. ■ Methods Preparation of HIBD modelsAll procedures conform to the Principles of Laboratory Animal Care issued by the National Institues of Health, with local laws and regulations, and were approved by the local Animal Care and Use Committee.A total of 40 SD newborn rats of both sexes, weighing 14.5 ± 3.2g, aged 7 days old were selected and purchased from Medical Experimental Animal Center of Guangdong Province. The preparation process of HIBD models was followed as previously described 21 . SD rats were fixed in supine position, and routine disinfection of neck was performed. Subsequently, a 3 mm longitudinal incision was made, and the right common carotid artery was isolated by vessel forceps. The carotid artery was ligated by 0 sterile line, and then the incision was ...

Research advances in the role of endogenous neurogenesis on neonatal hypoxic-ischemic brain damage

“…Hyperbaric oxygen therapy, reported to have neuroprotective effects in multiple neurological disorders, is now being evaluated as a novel Adjuvant therapy to clinical management of neonatal HIBD ( 73 ). This technique restores the blood-brain barrier via cerebral vasoconstriction, decreasing cerebral blood flow and reducing cerebral hematoma, and simultaneously increasing oxygen supply to ischemic tissues ( 74 ). A meta-analysis study ( 75 ) pointed out that Hyperbaric oxygen therapy significantly improved the total efficiency of treatment for neonatal HIE patients and reduced the risk of sequelae, however, the underlying mechanism is not clear.…”

Complementary Therapy with Traditional Chinese Medicine for Neonatal Hypoxic Ischemic Encephalopathy

“…These results suggest that Xuefu Zhuyu Decoction might protect neuron cells after HI injury by up-regulating NGF expression [82]. NGF is a neurotrophy, which can support the differentiation and survival of neuron cells and have anti-apoptotic and anti-oxidative effect which is showed to have beneficial effects on neonatal HIE rat [83].…”

Hyperbaric Oxygen Improves Cerebral Ischemia/Reperfusion Injury in Rats Probably via Inhibition of Autophagy Triggered by the Downregulation of Hypoxia-Inducing Factor-1 Alpha

“…A large number of animal experiments and clinical trials have shown that HBO alleviates infarct size and oedema and reduces the permeability of the blood-brain barrier, which has a positive effect on ischemic stroke [13][14][15][16]. Spatial memory has been effectively improved in rats with ischemic hypoxic brain injury, and this improvement may be attributable to the preservation of damaged cortical and hippocampal cells [17]. Mechanistically, the effect of HBO may be related to the suppression of oxidative stress, inflammation, and cell death after ischemic stroke [18].…”