Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity

Syafriza, Dharli; Sutadi, Heriandi; Primasari, Ameta; Siregar, Yahwardiah

doi:10.1590/pboci.2021.004

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…It was then incubated in an aerobic atmosphere at 37 0 C for 24 h. The film residue was separated by centrifuging 3000xg for 10 min, and the supernatant was considered living bacterial cells. Furthermore, it was measured by spectrophotometry with a wavelength of 600 nm [25].…”

Section: -11-antibacterial Assaymentioning

confidence: 99%

Characteristics and Antibacterial Properties of Film Membrane of Chitosan-Resveratrol for Wound Dressing

et al. 2023

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The research aimed to evaluate the film membrane of Nano Chitosan Resveratrol (NCHR) for biological, physicochemical, and antibacterial properties. Psychochemically, the functional groups of chitosan compounds were examined by FTIR, chemical compounds by GCMS, and the morphology of chitosan and chemical elements by SEM-EDS. Biologically, the characteristics of NCHR were examined by solubility, swelling, permeability, and biodegradation tests. Meanwhile, the antibacterial properties were examined for inhibition of Porphyromonas gingivalis (P. gingivalis) ATCC 33277 by Minimal inhibition concentration (MIC) and growth assessment by spectrophotometry. Nano Chitosan (NCH) has appeared at 1033.85 cm-1 as a sharp peak indicating the P=O group and contains anti-toxicity compounds (Ethane, 1,1-diethoxy- (CAS) 1,1-Diethoxye) is 81.06% and antioxidant compounds Limonene is (1.28%). In addition, NCH has chemical elements, Oxygen Weight (69.4%), calcium (19.7%), magnesium (6.6%), and phosphorus (4.3%). NaCl 0.9%, PBS, and Aquades. In addition, it has an excellent index of water vapor transmission rate (WVTR) in all solvents (R2³ 0.95). The NCHR membrane film is bacteriostatic (≤ 300 CFU/mL) with each value of Minimal Inhibition Concentration (MIC) >15 mm. The Nano chitosan contains antitoxic, antioxidant, and antibacterial compounds with high oxygen elements. The film membrane of nano chitosan resveratrol can maintain the stability of changes in pH with a very high solubility index, swelling index, and WVTR index, as well as good biodegradation and antibacterial properties. Doi: 10.28991/ESJ-2023-07-03-012 Full Text: PDF

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Section: -11-antibacterial Assaymentioning

confidence: 99%

Characteristics and Antibacterial Properties of Film Membrane of Chitosan-Resveratrol for Wound Dressing

et al. 2023

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“…In complex oral environment, the formation of dental biofilm was also affected by saliva secreted (De Foggi et al 2018). Syafriza et al (2021) found that saliva inhibited the growth and biofilm formation of S. mutans. Silva et al (2012) reported that artificial saliva could affect the biofilm formation of C. albicans.…”

Section: Introductionmentioning

confidence: 99%

“…Syafriza et al . (2021) found that saliva inhibited the growth and biofilm formation of S. mutans . Silva et al .…”

Section: Introductionmentioning

confidence: 99%

Optimization of environmental factors in a dual in vitro biofilm model of Candida albicans–Streptococcus mutans

Zhang

et al. 2022

Letters in Applied Microbiology

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The biofilm formation of Streptococcus mutans–Candida albicans is an important virulence factor for dental caries. The purpose of this study was to determine the effect of some environmental conditions on the biofilm formation like inoculation concentration, temperature, sugar, amino acid, metal ions and saliva, and then establish a persistent in vitro biofilm model for further research. Based on the single factor experiment, the factors participating in the biofilm formation including sugar, inoculation concentration, and saliva increased the biofilm mass, while amino acid, metal ions, temperatures reduced biofilm mass. Optimal conditions for biofilm formation were the inoculation dosage of S. mutans and C. albicans of 108 and 107, respectively, the addition of 0·3 g l−1 sucrose and sterile saliva. These results contribute to a deep understanding of the factors involved in oral biofilm formation of the important cariogenic pathogen S. mutans and the opportunistic pathogen C. albicans to study better for biofilm and promote the design of new therapeutic approaches. The present research also provides a model for evaluating the therapeutic potential for drugs in the future.

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Chemical compounds Anti-bacterial of Citrus aurantifolia Ethanol Extract to Inhibit the Early Biofilm Formation and Growth of Enterococcus faecalis Root Canal Isolate

Asmah

Suniarti

Bachtiar

et al. 2022

RJPT

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Enterococcus faecalis (E. faecalis) is a gram-positive commensal bacterium involved in the pathogenesis of dental root canal infections. Citrus aurantifolia can inhibit the adhesion and growth of E. faecalis. The study aims to evaluate the Antibacterial properties of Citrus aurantifolia ethanol extract to Inhibit the early biofilm formation and growth of Enterococcus faecalis Root Canal Isolate. The necrotic tissue of the root canal from infected patients was cultured by E. faecalis and E. faecalis ATCC 29212 (bacteria control) on ChromAgar medium. The inhibition of E. faecalis biofilm was measured using a violet crystal assay. In contrast, confirmation of E. faecalis growth from planktonic and biofilm masses was tested using the Total plate count assay, and data were analyzed using the Kruskal-Wallis test. All concentrations of ethanol extract of Citrus aurantifolia showed their potential in inhibiting early phase (18 hours) biofilm formation and growth of E. faecalis clinical isolates and ATCC 29212 from planktonic and biofilm masses in vitro. At concentrations 0.5, 2, 6, and 12% have a potent biofilm inhibition, and concentrations of 14% and 16% are moderate (p>0.05:0.091). Extracts with concentrations of 0.5, 2, and 6% were bacteriostatic against the clinical isolate of E. faecalis, and ATCC isolates from planktonic, while concentrations of 12, 14, and 16% were bactericidal (p>0.05:0.548). Specifically, E. faecalis clinical isolates from biofilm masses were only bacteriostatic (p<0.05;0.01). The inhibition of the biofilm from both E. faecalis and the growth of the colony was not significantly different (p>0.05:0.753) and does not positively correlate (r = - 0.095). Citrus aurantifolia has more potent biofilm inhibition against E. faecalis ATCC 29212 (100%) than E. faecalis clinical isolate (75%). The bacteriostatic and bacteriocidal properties of extracts against the E. faecalis from planktonic are more susceptible than those from biofilm mass sources.

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Spectrophotometric Analysis of Streptococcus mutans Growth and Biofilm Formation in Saliva and Histatin-5 Relate to pH and Viscosity

Cited by 3 publications

References 34 publications

Characteristics and Antibacterial Properties of Film Membrane of Chitosan-Resveratrol for Wound Dressing

Characteristics and Antibacterial Properties of Film Membrane of Chitosan-Resveratrol for Wound Dressing

Optimization of environmental factors in a dual in vitro biofilm model of Candida albicans–Streptococcus mutans

Chemical compounds Anti-bacterial of Citrus aurantifolia Ethanol Extract to Inhibit the Early Biofilm Formation and Growth of Enterococcus faecalis Root Canal Isolate

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