Protein Profiles of Individuals with Erosive Tooth Wear

Abstract

To determine if protein profiles identified in saliva could be used to determine risk and severity of erosive tooth wear. Material and Methods: Three types of saliva sampling were performed to obtain saliva from 34 18-year old individuals that received regular dental checkups , along with clinical status of the dentition and risk factor related to erosive tooth wear using the VEDE scale. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample to test for association with erosive tooth wear status. Results: There were no differences in the number of detectable proteins sourced from the parotid gland, nor the unstimulated and stimulated whole saliva. Five out of the 34 individuals had no signs of erosive tooth wear despite an acidic diet and were more likely to have proteins with molecular weight smaller than 1 KDa (p=0.03). Conclusion: There is potential for the use of protein profiling to determine risks for erosive tooth wear.

Risk factors of tooth wear in permanent dentition: A scoping review

“…Among all factors associated with TW, the sociodemographic factors were the most frequently studied in this scoping review (68.9% of included studies, n = 188) 2–5,20–197 . Sex and age represented 58.6% and 46.9% of studies in this category (Table 1).…”

“…Medical history was the second most frequently studied factor (67% of included studies; n = 183), 2,3,5,22–24,26–35,38,40,42–44,47,49–51,53,56–58,60–64,68–70,74,75,77,78,80,81,83,85,87,90–92,94–96,98,101,102,104–108,110,111,113,114,119–130,132,136–138,140–144,147,148,151,153–157,159,161,162,166,167,169,172,173,176–178, 180,181,185,188–190,194,196,197,199–256 and 27 studies were exclusively dedicated to this topic. The influence of the presence of GORD, stomach upset or heartburn was the most studied factor from this category, with 35.2% of studies included in this scoping review (96 out of 273).…”