Chemical Composition and Antibacterial Effect of Plantago Major Extract on Periodontal Pathogens

Abstract

To determine the in vitro antibacterial effect of different concentrations of the ethanol extract of Plantago major (plantain) on Porphyromonas gingivalis and Fusobacterium nucleatum. Material and Methods: Bacterial susceptibility tests were used in conjunction with the agar diffusion test and the minimum inhibitory concentration (MIC) test using the broth macrodilution technique. Results: Different concentrations of ethanol extract (25%, 50%, 75% and 100%) dissolved in 70% ethanol were used, with a positive control (0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride) and a negative control (70% alcohol). The extracts at 75% and 100% showed inhibition halos against both strains studied. With 0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride, inhibition halos averaged 14.9 mm, in contrast to 70° alcohol, where no bacterial inhibition was observed. The MIC was 50% for both species. Conclusion: The ethanol extract of Plantago major presents an in vitro antibacterial effect on Porphyromonas gingivalis, they may have potential applications in food and pharmaceutical products.

Uso de solução de Plantago major para o controle do biofilme dental: relato de caso

“…Verificou-se ação semelhante da planta com a clorexidina, tanto para a K. pneumoniae quanto para a C. tropicalis (Santos et al, 2007 (Trindade et al, 2018). Efeitos significativos foram verificados contra a P. gingivalis (Buffon et al, 2001;Freitas et al, 2002;Pensantes-Sangay et al, 2020;Valverde & Carolina, 2017), bactéria conhecida por estar associada a doença periodontal (Bezerra et al, 2020). Contudo a literatura não é unânime, estudo como o de Sharma et al (2016) não verificaram efetividade da Plantago major frente a P. gingivalis.…”

“…Possui atividades farmacológicas como: anti-inflamatória, antifúngica, analgésica, antibiótica, antivirais, antileucêmica e de cicatrização de feridas (Barreto, Costa Feitosa, Araújo, Chagas, & Costa, 2005;Brandão, Cosenza, Moreira, & Monte-Mor, 2006;Kantawong et al, 2017;Sharma et al, 2016;Shirley, Windsor, Eckert, & Gregory, 2017). Também é utilizada no tratamento de inflamações bucais (gengivites, periodontites e parotidites) e da orofaringe, como agente antibacteriano, antifúngico e antibiofilme (Bezerra et al, 2020;Buffon, Lima, Galarda, & Cogo, 2001;Flores, Gamba, & Coppola, 2009;Freitas et al, 2002;Pensantes-Sangay et al, 2020;Santos, Slusarz, Junior, & Schwartz, 2007;Souza et al, 2020;Trindade et al, 2018). A solução de Plantago major pode ainda ser indicada para tratamento de mucosites orais em pacientes com câncer, que estejam sob tratamento de radioterapia e quimioterapia (Cabrera-Jaime et al, 2018).…”

Identification of Proteins Associated with the Formation of Oral Biofilms

“…Therefore, F. nucleatum plays a crucial role as a bridge in the development of oral biofilms that are associated with periodontitis. Therefore, the identification of proteins that could inhibit the incorporation of F. nucleatum in oral biofilms could reduce its pathogenic potential [28][29][30][31][32].…”

Efectividad de la clorhexidina y aceites esenciales asociados al raspado y alisado radicular en el tratamiento de periodontitis crónica

“…S caling and root planing is a procedure by which plaque and calculi are removed from supragingival and subgingival tooth surfaces; root planing, consequently, removes calculi and adhered portions of residual cementum from the roots to create a smooth and clean surface. This treatment restores periodontal health and completely removes the elements that produce gingival inflammation; it also eliminates microorganisms and leads to a change in subgingival plaque composition (20).…”