In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue

Catão, Maria Helena Chaves de Vasconcelos; Batista, Ana

doi:10.1590/pboci.2020.073

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…Because of the role of biofilm formations in the successful establishment of infection, its destruction is an important way of curbing gram-positive bacterial infections. PDT has been shown to reduce the formation of biofilm in various studies [ 26 ]. We have also shown that PDT reduces the formation of biofilms following the use of Hypocrellin B and light [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Aloe-Emodin-Mediated Photodynamic Therapy Attenuates Sepsis-Associated Toxins in Selected Gram-Positive Bacteria In Vitro

Odhiambo

Liu

2021

J. Microbiol. Biotechnol.

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Sepsis is an acute inflammatory response that leadsto life-threatening complications if not quickly and adequately treated. Cytolysin, hemolysin, and pneumolysin are toxins produced by grampositive bacteria and are responsible for resistance to antimicrobial drugs, cause virulence and lead to sepsis. This work assessed the effects of aloe-emodin (AE) and photodynamic therapy (PDT) on sepsis-associated gram-positive bacterial toxins. Standard and antibiotic-resistant Enterococcus faecalis, Staphylococcus aureus, and Streptococcus pneumonia bacterial strains were cultured in the dark with varying AE concentrations and later irradiated with 72 J/cm -2 light. Colony and biofilm formation was determined. CCK-8, Griess reagent reaction, and ELISA assays were done on bacteriainfected RAW264.7 cells to determine the cell viability, NO, and IL-1β and IL-6 pro-inflammatory cytokines responses, respectively. Hemolysis and western blot assays were done to determine the effect of treatment on hemolysis activity and sepsis-associated toxins expressions. AE-mediated PDT reduced bacterial survival in a dose-dependent manner with 32 μg/ml of AE almost eliminating their survival. Cell proliferation, NO, IL-1β, and IL-6 cytokines production were also significantly downregulated. Further, the hemolytic activities and expressions of cytolysin, hemolysin, and pneumolysin were significantly reduced following AE-mediated PDT. In conclusion, combined use of AE and light (435 ± 10 nm) inactivates MRSA, S. aureus (ATCC 29213), S. pneumoniae (ATCC 49619), MDR-S. pneumoniae, E. faecalis (ATCC 29212), and VRE (ATCC 51299) in an AE-dose dependent manner. AE and light are also effective in reducing biofilm formations, suppressing proinflammatory cytokines, hemolytic activities, and inhibiting the expressions of toxins that cause sepsis.

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Section: Discussionmentioning

confidence: 99%

Aloe-Emodin-Mediated Photodynamic Therapy Attenuates Sepsis-Associated Toxins in Selected Gram-Positive Bacteria In Vitro

Odhiambo

Liu

2021

J. Microbiol. Biotechnol.

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BOPHY‐Fullerene C₆₀ Dyad as a Photosensitizer for Antimicrobial Photodynamic Therapy

Lopez

Sarotti

Martínez

et al. 2021

Chemistry A European J

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A novel BOPHY–fullerene C60 dyad (BP‐C60) was designed as a heavy‐atom‐free photosensitizer (PS) with potential uses in photodynamic treatment and reactive oxygen species (ROS)‐mediated applications. BP‐C60 consists of a BOPHY fluorophore covalently attached to a C60 moiety through a pyrrolidine ring. The BOPHY core works as a visible‐light‐harvesting antenna, while the fullerene C60 subunit elicits the photodynamic action. This fluorophore–fullerene cycloadduct, obtained by a straightforward synthetic route, was fully characterized and compared with its individual counterparts. The restricted rotation around the single bond connecting the BOPHY and pyrrolidine moieties led to the formation of two atropisomers. Spectroscopic, electrochemical, and computational studies disclose an efficient photoinduced energy/electron transfer process from BOPHY to fullerene C60. Photodynamic studies indicate that BP‐C60 produces ROS by both photomechanisms (type I and type II). Moreover, the dyad exhibits higher ROS production efficiency than its individual constitutional components. Preliminary screening of photodynamic inactivation on bacteria models (Staphylococcus aureus and Escherichia coli) demonstrated the ability of this dyad to be used as a heavy‐atom‐free PS. To the best of our knowledge, this is the first time that not only a BOPHY–fullerene C60 dyad is reported, but also that a BOPHY derivative is applied to photoinactivate microorganisms. This study lays the foundations for the development of new BOPHY‐based PSs with plausible applications in the medical field.

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Sodium copper chlorophyll mediated photodynamic treatment inactivates Escherichia coli via oxidative damage

Zhang

Qin²,

Wang³

et al. 2022

Food Research International

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In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue

Abstract: How to cite this article: Catão MHCV, Batista ALA. In vitro evaluation of the antibacterial effect of photodynamic therapy with methylene blue. Pesqui Bras Odontopediatria Clín Integr. 2020; 20:e4980.

Cited by 4 publications

References 23 publications

Aloe-Emodin-Mediated Photodynamic Therapy Attenuates Sepsis-Associated Toxins in Selected Gram-Positive Bacteria In Vitro

Aloe-Emodin-Mediated Photodynamic Therapy Attenuates Sepsis-Associated Toxins in Selected Gram-Positive Bacteria In Vitro

BOPHY‐Fullerene C₆₀ Dyad as a Photosensitizer for Antimicrobial Photodynamic Therapy

Sodium copper chlorophyll mediated photodynamic treatment inactivates Escherichia coli via oxidative damage

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In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue

Abstract: How to cite this article: Catão MHCV, Batista ALA. In vitro evaluation of the antibacterial effect of photodynamic therapy with methylene blue. Pesqui Bras Odontopediatria Clín Integr. 2020; 20:e4980.

Cited by 4 publications

References 23 publications

Aloe-Emodin-Mediated Photodynamic Therapy Attenuates Sepsis-Associated Toxins in Selected Gram-Positive Bacteria In Vitro

Aloe-Emodin-Mediated Photodynamic Therapy Attenuates Sepsis-Associated Toxins in Selected Gram-Positive Bacteria In Vitro

BOPHY‐Fullerene C60 Dyad as a Photosensitizer for Antimicrobial Photodynamic Therapy

Sodium copper chlorophyll mediated photodynamic treatment inactivates Escherichia coli via oxidative damage

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Product

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BOPHY‐Fullerene C₆₀ Dyad as a Photosensitizer for Antimicrobial Photodynamic Therapy