Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model

Abstract

In this study, the exopolysaccharides (EPSs)-producing L. plantarum YW11 was initially evaluated for its tolerance to simulated artificial gastric juice and bile salts, and cell surface hydrophobicity. Subsequent study using a HT-29 tumor-burdened nude mouse model showed that the EPS had potent immunomodulatory and antitumor activities. Administration of the EPS had no influence on body weight of the mice, and it could significantly prevent HT-29 tumor cells induced acute liver and kidney damages. The purified EPS at a dose of 5 mg/mL was able to enhance effectively the killing activities of splenic natural killer cells and cytotoxic T lymphocyte, and to stimulate the secretion of serum cytokines IL-2 and TNF-α. As evidenced by a cell culture assay, the EPS exhibited obvious inhibition on growth of colon cancer HT-29 cells treated with 600 μg/mL of the EPS for 72 h with an inhibition rate of 58.02%. Administration of the EPS obviously inhibited growth of HT-29 xenograft tumor to an extent near that of the positive control (5-Fu, 25 mg/kg bw). Therefore, the results of this study indicated the important roles of the EPS in the potential probiotic functions of the strain.

The impact of Apocynum venetum tea flavonoids on G422 glioma in vivo

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Effects of microbial preparation on production performance and rumen microbial communities of goat

“…The exopolysaccharides (EPSs)-producing L. plantarum YW 11 has potent immunomodulatory and antitumor activities. EPS prevented HT-29 tumor cells induced acute liver and kidney damages significantly, and promoting secretion of cytokines IL-2 and TNF-α in mice (Zhang et al, 2022). Lactobacillus plantarum SCS2 obviously protected against AFB1-induced oxidative stress.…”

Production of Conjugated Linoleic Acid (CLA) by <em>Lactiplantibacillus plantarum</em>: A Review With Emphasis on Fermented Foods

“…The results showed that L. plantarum YW11 is able to convert LA into a CLA isomer (rumenic acid) and stearic acid, at different concentrations of LA (also in this case1-10 % w/v) [121]. L. plantarum YW11, isolated from kefir from Tibet, has been found to possess antimicrobial, anticancer, antioxidant, and immuno-regulatory activities [136,137].…”