Preparation of total triterpenoids from Antrodia cinnamomea fermentation mycelium and their in vitro inhibitory effects on hepatocellular carcinoma

Abstract

Total triterpenoids from Antrodia cinnamomea fermentation mycelium (TT-ACFM) were prepared, and their in vitro inhibitory effects on hepatocellular carcinoma were investigated. Human hepatocellular carcinoma HepG2 cells were incubated with TT-ACFM with concentration of 0 (control), 12.5, 25, 50, 100 and 200 μg/mL, respectively. The proliferation of cell was detected using CCK-8 method. The apoptosis of cells was detected by flow cytometry. The migration and invasion of cells was determined using Transwell chamber. The level of reactive oxygen species (ROS) in cells was determined using DCFH-DA method. The expressions of matrix metalloproteinse-2 (MMP-2), matrix metalloproteinse-9 (MMP-9) and vascular endothelial growth factor (VEGF) protein in cells were detected by western blot assays. Results indicate that, TT-ACFM can not only inhibit the proliferation of HepG2 cells and promote their apoptosis, but also inhibit their invasion and invasion. The mechanism may be related to its increase of ROS and down-regulation of MMP-2, MMP-9 and VEGF expression in cells.

Comparative Transcriptomic Analyses Propose the Molecular Regulatory Mechanisms Underlying 1,8-Cineole from Cinnamomum kanehirae Hay and Promote the Asexual Sporulation of Antrodia cinnamomea in Submerged Fermentation

“…Antrodia camphorate or Taiwanofungus camphoratus) is a rare and valuable edible and medicinal fungus that belongs to the phylum Basidiomycetes and family Polyporaceae [1,2]. A. cinnamomea performs various types of bioactivities, such as hepatoprotective, antitumor, anti-inflammatory, antiviral, vasodilation-inhibiting, hypoglycemic, and immunoregulation [2][3][4]. Four main artificial culture processes for A. cinnamomea have been used: wood culture, plate culture, solid-state fermentation, and submerged fermentation (SmF).…”