Efeitos de diferentes concentrações de nitrogênio no crescimento de Aechmea blanchetiana (Baker) L.B. Sm. cultivada in vitro

Kanashiro, Shoey; Ribeiro, Roberval de Cassia Salvador; Gonçalves, Antônio Natal; Dias, Carlos Tadeu dos Santos; Jocys, T.

doi:10.1590/s2236-89062007000100003

Cited by 30 publications

(22 citation statements)

References 10 publications

(10 reference statements)

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“…Indivíduos de A. bromeliifolia (Rudge) Baker apresentaram maior crescimento da parte aérea e mais massa seca de raízes e da parte aérea em meio MS contendo de zero a 50% da concentração original de macronutrientes (Fráguas et al 2002). Para A. blanchetiana, o aumento gradativo da concentração de sais nitrogenados no meio MS levou ao decréscimo linear dos parâmetros morfológicos avaliados, sendo o meio com a menor concentração de sais nitrogenados (12,5%) considerado adequado para o cultivo desta bromélia (Kanashiro et al 2007). Em um estudo de micropropagação de Alcantarea imperialis (Carriere) Harms em meio MS, os tratamentos com 25 e 100% de sais nitrogenados proporcionaram o maior crescimento dos indivíduos .…”

Section: Número De Raízesunclassified

Baixas concentrações de macronutrientes beneficiam a propagação in vitro de Vriesea incurvata (Bromeliaceae), uma espécie endêmica da Floresta Atlântica, Brasil

2016

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A cultura in vitro é uma ferramenta eficiente para a propagação de plantas de importância ecológica e econômica e permite o entendimento acerca de aspectos ecofisiológicos das espécies. Este estudo teve por objetivo avaliar a influência de diferentes concentrações de macronutrientes sobre o desenvolvimento in vitro e a sobrevivência ex vitro de plântulas de Vriesea incurvata, visando à conservação desta bromélia epifítica endêmica da Floresta Atlântica. A germinação in vitro foi avaliada aos 60 dias e as plântulas foram cultivadas por 180 dias em meio MS com 25 ou 50% dos macronutrientes, 25 ou 50% dos sais nitrogenados, bem como com 100% da formulação original do meio. As sementes apresentaram 95% de germinação. Em todos os tratamentos, houve 100% de sobrevivência das plântulas cultivadas in vitro. A redução de todos os macronutrientes ou dos sais nitrogenados mostrou-se benéfica, proporcionando maior comprimento da parte aérea e da raiz maior, maior número de folhas e de raízes, bem como maior massa fresca, além de ter propiciado 97% de sobrevivência das plântulas aclimatizadas ex vitro. Os dados obtidos permitem o estabelecimento de um protocolo de propagação in vitro de V. incurvata, com o objetivo de sua futura reintrodução no habitat natural. Palavras-chave: bromélias, conservação, micropropagação, nutrição mineral. AbstractIn vitro culture is an efficient tool for the propagation of plants of ecological and economic importance and allows the understanding about ecophysiological aspects of the species. The objective of this study was to assess the influence of different macronutrient concentrations on in vitro development and ex vitro survival of Vriesea incurvata plantlets, aiming the conservation of this endemic epiphytic bromeliad from the Atlantic Forest. The in vitro germination was assessed at 60 days and the plantlets were cultivated for 180 days on MS medium with 25 or 50% of the macronutrients, 25 or 50% of the nitrogen salts, or with 100% of the original formulation of the medium. The seeds showed 95% germination. In all treatments, there was 100% survival of in vitro cultivated plantlets. The reduction of all macronutrients or of the nitrogen salts was beneficial, and permitted a greater length of the aerial part and of the major root, a higher number of leaves and roots, and more fresh mass, as well as allowed 97% survival of ex vitro acclimatized plantlets. The obtained data allow the establishment of a protocol for in vitro propagation of V. incurvata, aiming its future reintroduction into the natural habitat.

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Section: Número De Raízesunclassified

Baixas concentrações de macronutrientes beneficiam a propagação in vitro de Vriesea incurvata (Bromeliaceae), uma espécie endêmica da Floresta Atlântica, Brasil

2016

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“…The formation of new buds was also negatively influenced by the increase in the amino acid concentration in the culture medium and the smallest average was obtained with 50 µM tyrosine. Nitrogen is a constituent of amino acids, nucleotides and coenzymes (Kanashiro et al 2007); therefore, there is a correlation between the availability of nitrogen and the formation of new organs. The presence of aromatic amino acids with hydroxyl groups, such as Ser and Tyr, in the nutrient solution could have affected the magnesium transporter activity (Garcia et al 2011), and thus, the development off the plant and the formation of new sprouts and buds ( Fig.…”

Section: In Vitro Culturing Of a Tenellamentioning

confidence: 99%

Pigment production and growth of Alternanthera plants cultured in vitro in the presence of tyrosine

Kleinowski

Brandão

Einhardt

et al. 2013

Braz. arch. biol. technol.

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“…Most substrates used for cultivation of orchids are poor in nutrients and/or present low capacity for supplying nutrients, making fertilization necessary under these conditions (Rego et al, 2000;Araujo et al, 2007). In addition, nutritional studies on orchids are more frequently about in vitro cultivation (Kanashiro et al, 2007;Stancato et al, 2008;Moraes et al, 2009;Rodrigues et al, 2012a,b).…”

Section: Introductionmentioning

confidence: 99%

Phosphorus-Zinc Interaction and Iron and Manganese Uptake in the Growth and Nutrition of Phalaenopsis (Orchidaceae)

Novais¹,

Novais

et al. 2016

Rev. Bras. Ciênc. Solo

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Visual symptoms of Zn deficiency, induced by excessive P applications, have been observed in commercial orchid nurseries. The supply of other metallic micronutrients, such as Fe and Mn, may also be inadequate in the plant due to high application rates of P. The aim of this study was to investigate this interaction in the nutrition of Phalaenopsis plants. Experimental treatments consisted of three P rates . Dry matter production was evaluated, as well as the levels of P, Zn, Fe, and Mn in both shoots and roots. Higher P rates induced higher shoot dry matter production. However, symptoms of Zn deficiency were observed in plants treated with the highest P rate in the absence of Zn. With increasing P rates, Zn concentrations in the shoots decreased more markedly than in roots, with accumulation of the element in the roots, indicating low Zn translocation to the shoot. A much higher Mn content in shoots (661 mg kg ) suggested that the species is highly tolerant to this micronutrient. The Fe concentrations in the plant were much higher than those indicated in the literature as critical levels for this genus.

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Efeitos de diferentes concentrações de nitrogênio no crescimento de Aechmea blanchetiana (Baker) L.B. Sm. cultivada in vitro

Cited by 30 publications

References 10 publications

Baixas concentrações de macronutrientes beneficiam a propagação in vitro de Vriesea incurvata (Bromeliaceae), uma espécie endêmica da Floresta Atlântica, Brasil

Baixas concentrações de macronutrientes beneficiam a propagação in vitro de Vriesea incurvata (Bromeliaceae), uma espécie endêmica da Floresta Atlântica, Brasil

Pigment production and growth of Alternanthera plants cultured in vitro in the presence of tyrosine

Phosphorus-Zinc Interaction and Iron and Manganese Uptake in the Growth and Nutrition of Phalaenopsis (Orchidaceae)

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