Cytotoxicity of separation orthodontic elastics

Santos, Rogério Lacerda dos; Pithon, Matheus Melo; Martins, Fernanda Otaviano; Romanos, Maria Teresa Villela

doi:10.1590/s2176-94512012000400021

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…Previously, the elastics were superficially washed with deionized water (Millipore, Bedford, Mass) for 5 seconds and sterilized on both sides with ultraviolet light (Labconco, Kansas City, Mo) for 30 minutes. [3][4][5]18 To verify the cell response to extreme situations, another three groups were included in the study: group cell control (CC), consisting of cells not exposed to any material; group positive control (C+), consisting of Tween 80; and group (negative control (C2), consisting of phosphate-buffered saline (PBS) solution in contact with the cells.…”

Section: Cytotoxicity Testmentioning

confidence: 99%

The influence of pH levels on mechanical and biological properties of nonlatex and latex elastics

Santos¹,

Pithon²,

Romanos³

2012

The Angle Orthodontist

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Objective: To evaluate the influence of pH levels on interarch elastics with regard to force decay and cytotoxicity. Materials and Methods: One nonlatex (NLAO) group and one latex (LAO) group were tested (n 5 10). Elastics were stretched to 25 mm and were held for 1, 6, 12, and 24 hours in artificial saliva solutions with pH levels of 5.0, 6.0, and 7.5. Force magnitudes were measured at 25 mm of activation. The cytotoxicity assay was performed using cell cultures (L929 mouse fibroblast cell line), which were subjected to the cell viability test with neutral red (''dye-uptake''). Force decay and cytotoxicity were assessed using analysis of variance, the Sidak method, and a Tukey's test. Results: The interactions between group, pH, and time showed no statistically significant differences (P 5 .29). When pH per time (P 5 .032) and group per time (P 5 .0009) were considered, these interactions showed statistically significant differences (P , .05). The pH did not interfere directly in the degradation results of the tested elastics. The cytotoxicity test showed that group LAO presented lower cell viability when compared with group NLAO over the course of the entire experiment. There was a gradual reduction in cell viability from 1 hour to 24 hours. A significant difference (P , .05) was found between the interactions group pH and the control group of cells, except between group NLAO at the time point of 1 hour at different pH values and at the time points of 6 and 12 hours with pH 5 (P . .05). Conclusions: No significant correlation between pH, force decay, and cytotoxicity was observed. (Angle Orthod. 2012;82:709-714.)

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Section: Cytotoxicity Testmentioning

confidence: 99%

The influence of pH levels on mechanical and biological properties of nonlatex and latex elastics

Santos¹,

Pithon²,

Romanos³

2012

The Angle Orthodontist

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“…Spectrophotometric essay allows rapid and reliable evidence of cell viability to be obtained based on the use of vital stain incorporated by viable cells [23][24][25][26] . In this study, neutral red dye was used, as it is widely used for identifying L-929 cell viability 23,[25][26] .…”

Section: Discussionmentioning

confidence: 99%

In vitro study of cytotoxicity of orthodontic elastomeric ligatures

et al. 2012

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This study investigated the cytotoxicity of crystal-coloured orthodontic elastomeric ligatures of polyurethane. Six ligatures from distinct manufactures were divided into 6 groups of 10 elastics each: Groups P1, P2, P3, P4, P5 and P6 (Polyurethane). The cytotoxicity essay was performed using L-929 line cells, which were submitted to the cell viability test with neutral red ("dye-uptake") at time intervals of 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with multiple comparisons and Tukey's test were used (p < .05). There were statistical differences (p < .05) in cell viability between Groups P1, P4, P2 and P3, and Groups P5 and P6 at 1 and 2 days. All elastomeric ligatures were considered suitable for clinical use. The hypothesis was accepted, the P5 and P6 elastomers and the processing route of injection molding for these ligatures showed the lowest cell viability, due the temperature and pressure distinct in the processing of these elastomers.

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“…The cell cultures have been used as part of a series of tests recommended for evaluating the biologic behavior of materials to be placed in contact with human tissues. 9,14,21 In this study, cytotoxicity tests were performed to evaluate the cytotoxicity of alginates. The L929 cell lineage (mouse fibroblasts) was used because these cells are frequently used in various studies in which the intention was to evaluate the cytotoxicity of materials for dental use.…”

Section: E3mentioning

confidence: 99%

Cytotoxicity of alginate for orthodontic use

Pithon

Santos

Martins³

et al. 2012

Dental Press J. Orthod.

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Objective: To evaluate the cytotoxicity of three different alginate impression materials for orthodontic use. Methods: Three different brands of alginate were divided into three groups, namely, Group JCO (Jeltrate Chromatic Ortho), OP (Orthoprint) and CO (Cavex Orthotrace). Three control groups were also included: Group C+ (positive control), consisting of detergent Tween 80; Group C-(negative control), consisting of PBS, and Group CC (cell control), consisting of cells not exposed to any material. After manipulating the materials according to the respective manufacturer instructions, samples were made with the use of silicon rings. Then the samples were immersed in Eagle's minimum essential medium (MEM) for 2 minutes. The supernatants were then removed and brought into direct contact with L929 fibroblasts. After exposure to the medium, the cells were incubated for 24 hours. Then 100 μl of 0.01% neutral red dye were added. The cells were incubated again for 3 hours so that the dye could be absorbed. After this 3-hour period, the cells were fixed to perform the viable cell count, using a spectrophotometer (BioTek, Winooski, Vermont, USA) at a wavelength of 492 nm. Results: Statistical differences were found when Groups CC and C-were compared with the other experimental groups. Group JCO had the highest cytotoxicity, followed by Groups OP and CO. Conclusion: Based on the results obtained in this work, it was concluded that all alginate impression materials are potentially cytotoxic. Objetivo: avaliar a citotoxicidade de três diferentes alginatos de uso ortodôntico. Métodos: foram avaliados três diferentes alginatos divididos em três grupos, denominados grupo JCO (Jeltrate Chromatic Ortho), OP (Orthoprint) e CO (Carrex Orthotrace). Três grupos controle também participaram: controle + (C+), constituído pelo detergente celular Tween 80; controle -(C-) PBS; e controle de célula (CC) onde as células não foram expostas a nenhum material. Após manipulação dos materiais, seguindo as orientações do fabricante, foram confeccionados corpos de prova utilizando-se anéis de silicone. Em seguida, esses foram imersos em meio mínimo essencial de Eagle (MEM) por 2min, onde, então, procedeu-se à remoção do sobrenadante e à colocação em contato com fibroblastos L929. Após contato com o meio, as células foram incubadas por mais 24h onde, então, foi adicionado o corante vermelho neutro a 0,01%. Passado esse período, foram fixadas e, então, realizada contagem de células viáveis em espectrofotômetro (BioTek, Vermont, EUA) em um comprimento de onda de 492nm. Resultados: os resultados demonstraram diferenças estatística entre os grupos CC e C-com os demais. O grupo experimental JCO mostrou-se com maior citotoxicidade, seguido pelos grupso OP e CO. Conclusões: pode-se concluir, com a realização desse trabalho, que todos os alginatos testados mostraram caráter citotóxico. Keywords Palavras-chave:Citotoxicidade celular anticorpo-dependente. Materiais para moldagem odontológica. Técnicas de cultura de células.

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Cytotoxicity of separation orthodontic elastics

Cited by 3 publications

References 17 publications

The influence of pH levels on mechanical and biological properties of nonlatex and latex elastics

The influence of pH levels on mechanical and biological properties of nonlatex and latex elastics

In vitro study of cytotoxicity of orthodontic elastomeric ligatures

Cytotoxicity of alginate for orthodontic use

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