Development and validation of a HPTLC method for analysis of Sunitinib malate

Hajmalek, Monireh; Goudarzi, Masoumeh; Ghaffari, Solmaz; Attar, Hossein; Mazlaghan, Mehrnoosh Ghanbari

doi:10.1590/s1984-82502016000400003

Cited by 19 publications

(11 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The abovementioned score for both HPLC techniques showed a lower AGREE score, suggesting that the reported methods are not greener compared with present HPTLC methods for the analysis of lenvatinib in both bulk and capsule formulation. As the HPTLC method was not reported for the estimation of lenvatinib, we have compared our developed methods with HPTLC methods developed for other similar drugs, namely, sorafenib tosylate and sunitinib malate. , For the stated HPTLC technique for sorafenib tosylate and sunitinib malate, Figure E,F exhibited AGREE tool scores of 0.62 and 0.65, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, the other four reported techniques (methods 3 to 6) were quantitatively analyzed for the use of reagents, and it was observed that very few renewable reagents were used for the analysis, and many of the reagents were not from renewable sources. The toxic solvents (dichloromethane, toluene, ammonia solution, and n -hexane) and toxic substances (ammonium acetate and ammonium bicarbonate) , were used in large volumes which were very harmful to the environment, operator, and aquatic life. Hence, a score of 0.5 is given for methods (3 to 6) by the AGREE tool.…”

Section: Resultsmentioning

confidence: 99%

“…Sharma et al (2021) described the HPTLC method for the estimation of sorafenib tosylate and chrysin . The HPTLC technique for analysis of sunitinib malate was developed and validated by Hajmalek et al Using 12 distinct GAC (SIGNIFICANCE) principles as given in the literature, the greenness score of all six methods (methods1 to 6) was evaluated and compared Table shows the greenness of solvents that are environmentally friendly and renewable and GSK solvent selection guide which is commonly used in GAC. − The use of aqueous solvents as a mobile phase is always a better option instead of using harmful organic solvents …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Development of Green RP- and Green NP-HPTLC Methods for Estimation of Lenvatinib and Comparative Evaluation by AGREE

Bang

Bhatt

2023

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Lenvatinib mesylate (LNB) is a potent anticancer drug used in the treatment of thyroid cancer. No green technique incorporating the use of green analytical chemistry (GAC) in HPTLC for LNB has been reported. The current study focuses on how GAC was used in the development and validation of a green HPTLC technique using LNB. The mobile phase used for RP-HPTLC was green solvents, namely, ethanol and water (60:40, v/v), and for a green NP-HPLTC technique, a 50:50 v/v of ethanol and ethyl acetate was used. For both methods, the detection was carried out at 243 nm. Both methods were validated for linearity, range, precision, accuracy, limit of detection, limit of quantification, sensitivity, and specificity. In the range of 200–2000 ng/spot for both the RP and NP, both techniques were proved to be linear. In comparison to NP-HPTLC, the RP-HPTLC exhibited greater sensitivity. It was concluded that the green RP-HPTLC technique was found to be more speedy, accurate, precise, and greener than green NP-HPTLC. Analytical greenness (AGREE) scores were estimated as 0.88 and 0.82, respectively, for the RP and NP (methods 1 and 2), which indicated a high level of greenness. We compared our developed methods with previously reported HPLC (for LNB) and HPTLC (for similar class drugs) methods showing AGREE scores of 0.58, 0.61, 0.62, and 0.65, which are considered poor AGREE scores when compared to our established green RP- and NP-HPTLC methods. Both of our methods (methods 1 and 2) demonstrated extreme greenness as compared to other reported methods, and these emerging environmentally friendly green methods should be explored in pharmaceutical industries to reduce the potential hazards of organic solvents on nature.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Development of Green RP- and Green NP-HPTLC Methods for Estimation of Lenvatinib and Comparative Evaluation by AGREE

Bang

Bhatt

2023

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…For the quality control (QC) of TKIs, it is absolutely necessary to use proper analytical techniques. The analytical methods mentioned in the literature for QC of TKIs in their dosage forms are HPLC and HPTLC [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ], voltammetry [ 28 ], spectrofluorometry [ 29 , 30 , 31 , 32 ] and spectrophotometry [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. The most accessible methodology is spectrophotometry, which is quite easy, the least expensive and available in most quality control laboratories.…”

Section: Introductionmentioning

confidence: 99%

Experimental and Computational Evaluation of Chloranilic Acid as an Universal Chromogenic Reagent for the Development of a Novel 96-Microwell Spectrophotometric Assay for Tyrosine Kinase Inhibitors

Darwish

Khalil

et al. 2021

Molecules

View full text Add to dashboard Cite

The tyrosine kinase inhibitors (TKIs) are chemotherapeutic drugs used for the targeted therapy of various types of cancer. This work discusses the experimental and computational evaluation of chloranilic acid (CLA) as a universal chromogenic reagent for developing a novel 96-microwell spectrophotometric assay (MW-SPA) for TKIs. The reaction resulted in an instantaneous formation of intensely purple colored products with TKIs. Spectrophotometric results confirmed that the reactions proceeded via the formation of charge-transfer complexes (CTCs). The physical parameters were determined for the CTCs of all TKIs. Computational calculations and molecular modelling for the CTCs were conducted, and the site(s) of interaction on each TKI molecule were determined. Under the optimized conditions, Beer’s law correlating the absorbances of the CTCs with the concentrations of TKIs were obeyed in the range of 10–500 µg/well with good correlation coefficients (0.9993–0.9998). The proposed MW-SPA fully validated and successfully applied for the determination of all TKIs in their bulk forms and pharmaceutical formulations (tablets). The proposed MW-SPA is the first assay that can analyze all the TKIs on a single assay system without modifications in the detection wavelength. The advantages of the proposed MW-SPA are simple, economic and, more importantly, have high throughput.

show abstract

“…HPTLC is an advanced form of instrumental TLC and it is generally used with an unmodified silica layer as stationary phase on precoated plates and slit-scanning densitometry with UV-vis light as the detection technique 13 . HPTLC has simplicity of procedure, efficiency with small amounts of sample, parallel analysis of samples, multiple nondestructive methods for detection, numerous options for developing solvents, low consumption of solvents, time and cost effectiveness (In fact, the expenses associated with providing solvents and maintenance are much lower in comparison with HPLC) 5 . These advantages enable this method to be an important alternative to other chromatographic techniques such as HPLC for determining thiamine in green beans.…”

Section: Introductionmentioning

confidence: 99%

Application of High Performance Thin Layer Chromatography-Densitometry and UV- Visible Spectrophotometry for the Simultaneous Determination of Thiamine in Green Beans

Asra¹,

Chandra²,

Zulharmita³

2018

IJCTR

View full text Add to dashboard Cite

Two methods are described for the simultaneous determination of thiamine in green beans. The green beans were grinded and thiamine was extracted as bases into distilled water, separated by the first method, HPTLC silica gel 60 F 254 plate using methanol: water: acetic acid: ammonia (5:4.5:0.5:0.75) as mobile phase followed by densitometry measurement of its spot. The second method, a highly sensitive colour reaction has been developed. Thiamine was reacted with bromothymol blue to form an ion association complex in a weak base aqueous solution in the presence of some solubilization agents such as polyvinyl alcohol and analyzed by using UV-visible Spectrophotometry. The solution was measured at a maximum absorbance length of 430.5 nm. The first method showed that the detector response was linear for concentrations between 100-500 g/ml (r=0.998). The limits of detection and quantitation were 33.7 g/ml and 113.1 g/ml, respectively. The second method was found to offer good linearity (18-26 g/ml, r=0.998) with 0.6 g/ml limit of detection and 1.9 g/ml limit of quantitation. Thiamine contents from both methods were analyzed. The result showed that average contents of thiamine from both methods were 0.0396% and 1.0009%, respectively. The two proposed methods were successfully applied to the determination of thiamine in green beans.

show abstract

Development and validation of a HPTLC method for analysis of Sunitinib malate

Cited by 19 publications

References 15 publications

Development of Green RP- and Green NP-HPTLC Methods for Estimation of Lenvatinib and Comparative Evaluation by AGREE

Development of Green RP- and Green NP-HPTLC Methods for Estimation of Lenvatinib and Comparative Evaluation by AGREE

Experimental and Computational Evaluation of Chloranilic Acid as an Universal Chromogenic Reagent for the Development of a Novel 96-Microwell Spectrophotometric Assay for Tyrosine Kinase Inhibitors

Application of High Performance Thin Layer Chromatography-Densitometry and UV- Visible Spectrophotometry for the Simultaneous Determination of Thiamine in Green Beans

Contact Info

Product

Resources

About