Beneficial effects of methanolic extract of Indigofera linnaei Ali. on the inflammatory and nociceptive responses in rodent models

Abstract

Indigofera linnaei Ali. (Tamil Name: Cheppu Nerinjil) belongs to the family Fabaceae, used for the treatment of various ailments in the traditional system of medicine. In the present study, the beneficial effects of methanol extract of whole plant of I. linnaei (MEIL) were evaluated on inflammation and nociception responses in rodent models. In vitro nitric oxide (NO), lipoxygenase (LOX) and cyclooxygense (COX) inhibitory activities were also performed to understand the mode of action. MEIL at the dose of 200 & 400 mg/kg, p.o. significantly inhibited carrageenan induced rat paw volume and reduced the weight of granuloma in cotton pellet granuloma model. The results obtained were comparable with the standard drug aceclofenac. The anti-nociceptive effect of MEIL in mice was evaluated in hot plate and acetic acid induced writhing model. The plant extract significantly reduced the number of writhes and the analgesic effect was higher than that of the standard drug aspirin. However, the extract fails to increase the latency period in hot plate method suggesting that the extract produce nociception by peripheral activity. The extract produced inhibitory effect on NO, LOX and COX in concentration dependent manner. The extract exhibited pronounced and selective COX-2 inhibition. Altogether, these results suggested that the methanol extract of Indigofera linnaei could be considered as a potential antiinflammatory and analgesic agent. Uniterms

Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats

“…A new isoflavone namely 7,8methylenedioxy-4'-methoxyisoflavone has been isolated from the entire plant of Indigofera linnaei (Rajendraprasad and Chakradhar, 2004). In our earlier studies, the plant extract exhibited a potent free radical scavenging and antioxidant activity (Kumar et al, 2011a), anticancer activity (Kumar et al, 2011b), antiinflammatory and analgesic activities via inhibition of nitric oxide, lipoxygenase and cyclooxygenase (Kumar et al, 2016).…”

“…Group II animals served as DEN control group, received vehicle. Group III animals were treated with standard drug silymarin at a dose of 200 mg/kg by oral route, whereas group IV and V animals were treated with MEIL at a dose of 200 and 400 mg/kg by oral route, respectively (Kumar et al, 2011b;Kumar et al, 2016). All the treatments were given for 16 weeks after the administration of DEN on 5 days per week.…”

The medicinal plant Calpurnia aurea leaves act as an anti-inflammatory source of 5-Lipoxygenase / Cyclooxygenase-2 dual inhibitors

“…According to modi ied method of (Bisht et al, 2014;Kumar et al, 2016) 160µl of 100mM sodium phosphate buffer (pH 8.0), 10µl of tested leaf extracts C. aurea and 20µl of soybean Lipoxygenases solution (167 U/ml) were mixed and allowed to incubated at 25 • C for 10 min. The reaction was then started by the addition of 10µl of the ω-6 fatty acids unsaturated fatty acid linoleic acid as a substrate in the form of sodium linoleic acid solution.…”

“…In vitro COX-1 and COX-2 inhibitory activity of various concentrations of (20-100µg/ml) organic leaf extracts C. aurea (CALPE, CALCH, CALAC and CALET) were determined according to modi ied procedure of (Bisht et al, 2014;Kumar et al, 2016) by monitored change in absorption at 405 nm by using UV-Vis spectrophotometer.…”

Φυτοχημική ανάλυση του φυτού Indigofera hirsuta (Leguminosae) και έλεγχοι αντιοξειδωτικής δράσης και αναστολής της ALR-1, ALR-2

“…Η αντιοξειδωτική ικανότητα συγκρίθηκε με γνωστές αναστολή της ενδοπεριτοναϊκής αύξησης από ασκίτη λόγω του σαρκώματος του Ehrlich (EAC) και ασκίτη λόγω του λεμφώματος του Dalton (DLA). Επίσης οδήγησε σε αύξηση του μέσου όρου ζωής και είχε προστατευτική δράση του αιμοποιητικού συστήματος..Αντιφλεγμονώδη δράση: Οι(Kumar et al, 2016), μελέτησαν τα ευεργετικά αποτελέσματα του μεθανολικού εκχυλίσματος ολόκληρου του φυτού κατά τη διάρκεια της φλεγμονώδους απόκρισης και της απόκρισης αλγαισθησίας σε τρωκτικά. Μελετήθηκε ταυτοχρόνως η in vitro αναστολή της ρίζας του μονοξειδίου του αζώτου, της λιποξυγονάσης (LOX) και της κυκλοξυγονάσης (COX) και βρέθηκε να είναι δοσοεξαρτώμενη.…”