Influence of papain in biofilm formed by methicillin-resistant Staphylococcus epidermidis and methicillin-resistant Staphylococcus haemolyticus isolates

Oliveira, Hanna Lara da Cruz Dinéas de; Fleming, Maria Emília Castro Kling de; Silva, Patricia Vollú; Paula, Geraldo Renato de; Futuro, Débora Omena; Velarde, Guillermo Coca; Esper, Luciana Maria Ramires; Teixeira, Lenise Arneiro

doi:10.1590/s1984-82502014000200005

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…Papain effects on functional groups, such as hydroxyl, amino, carbonyl, and phosphoryl reduced biofilm formation. Several studies evaluated the ability of papain to inhibit the formation of Methicillin-resistant S. epidermidis (MRSE) and methicillin-resistant S. haemolyticus (MRSHa) biofilms; in addition, papain was able to disrupt mature biofilms made by MRSE (Oliveira, Fleming et al, 2014). On the other hand, proteases reduced oral biofilm in elderly subjects, suggesting that protease digests fimbriae and inhibits biofilm formation; thus, papain and trypsin reduce multispecies biofilm (Mugita, Nambu, Takahashi, Wang, & Komasa, 2017).…”

Section: Papainmentioning

confidence: 99%

Advanced strategies for combating bacterial biofilms

Sharahi

Azimi

Shariati

et al. 2019

Journal Cellular Physiology

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Biofilms are communities of microorganisms that are formed on and attached to living or nonliving surfaces and are surrounded by an extracellular polymeric material. Biofilm formation enjoys several advantages over the pathogens in the colonization process of medical devices and patients' organs. Unlike planktonic cells, biofilms have high intrinsic resistance to antibiotics and sanitizers, and overcoming them is a significant problematic challenge in the medical and food industries. There are no approved treatments to specifically target biofilms. Thus, it is required to study and present innovative and effective methods to combat a bacterial biofilm. In this review, several strategies have been discussed for combating bacterial biofilms to improve healthcare, food safety, and industrial process.

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Section: Papainmentioning

confidence: 99%

Advanced strategies for combating bacterial biofilms

Sharahi

Azimi

Shariati

et al. 2019

Journal Cellular Physiology

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“…In Brazil, three products are available: papain, collagenase, and fibrinolysin/DNase ( Table 4 ). 67 , 68 , 69 , 70 , 71 …”

Section: General Principles For Local Management Of Chronic Ulcersmentioning

confidence: 99%

Consensus on the diagnosis and management of chronic leg ulcers - Brazilian Society of Dermatology

Abbade

Frade

Pegas³

et al. 2020

Anais Brasileiros de Dermatologia

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Background Chronic leg ulcers affect a large portion of the adult population and cause a significant social and economic impact, related to outpatient and hospital care, absence from work, social security expenses, and reduced quality of life. The correct diagnosis and therapeutic approach are essential for a favorable evolution. Objective To gather the experience of Brazilian dermatologists, reviewing the specialized literature to prepare recommendations for the diagnosis and treatment of the main types of chronic leg ulcers. Methods Seven specialists from six university centers with experience in chronic leg ulcers were appointed by the Brazilian Society of Dermatology to reach a consensus on the diagnosis and therapeutic management of these ulcers. Based on the adapted DELPHI methodology, relevant elements were considered in the diagnosis and treatment of chronic leg ulcers of the most common causes; then, the recent literature was analyzed using the best scientific evidence. Results The following themes were defined as relevant for this consensus - the most prevalent differential etiological diagnoses of chronic leg ulcers (venous, arterial, neuropathic, and hypertensive ulcers), as well as the management of each one. It also included the topic of general principles for local management, common to chronic ulcers, regardless of the etiology. Conclusion This consensus addressed the main etiologies of chronic leg ulcers and their management based on scientific evidence to assist dermatologists and other health professionals and benefit the greatest number of patients with this condition.

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“… 17 Though the bactericidal activities of bromelain and papain against S. aureus have not been clearly elucidated, papain showed no antibacterial effect on 138 isolates of Staphylococcus epidermidis and Staphylococcus haemolyticus after 24 hours of treatment. 18 …”

Section: Resultsmentioning

confidence: 99%

Enzymatic degradation of in vitro Staphylococcus aureus biofilms supplemented with human plasma

Watters

Burton

Kirui

et al. 2016

IDR

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Enzymatic debridement is a therapeutic strategy used clinically to remove necrotic tissue from wounds. Some of the enzymes utilized for debridement have been tested against bacterial pathogens, but the effectiveness of these agents in dispersing clinically relevant biofilms has not been fully characterized. Here, we developed an in vitro Staphylococcus aureus biofilm model that mimics wound-like conditions and employed this model to investigate the antibiofilm activity of four enzymatic compounds. Human plasma at concentrations of 0%–50% was supplemented into growth media and used to evaluate biofilm biomass accumulation over 24 hours and 48 hours in one methicillin-sensitive and five methicillin-resistant strains of S. aureus. Supplementation of media with 10% human plasma resulted in the most robust biofilms in all six strains. The enzymes α-amylase, bromelain, lysostaphin, and papain were then tested against S. aureus biofilms cultured in 10% human plasma. Quantification of biofilms after 2 hours and 24 hours of treatment using the crystal violet assay revealed that lysostaphin decreased biomass by up to 76%, whereas α-amylase, bromelain, and papain reduced biomass by up to 97%, 98%, and 98%, respectively. Scanning electron microscopy confirmed that the dispersal agents detached the biofilm exopolysaccharide matrix and bacteria from the growth surface. Lysostaphin caused less visible dispersal of the biofilms, but unlike the other enzymes, induced morphological changes indicative of bacterial cell damage. Overall, our results indicate that use of enzymes may be an effective means of eradicating biofilms and a promising strategy to improve treatment of multidrug-resistant bacterial infections.

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Influence of papain in biofilm formed by methicillin-resistant Staphylococcus epidermidis and methicillin-resistant Staphylococcus haemolyticus isolates

Cited by 10 publications

References 27 publications

Advanced strategies for combating bacterial biofilms

Advanced strategies for combating bacterial biofilms

Consensus on the diagnosis and management of chronic leg ulcers - Brazilian Society of Dermatology

Enzymatic degradation of in vitro Staphylococcus aureus biofilms supplemented with human plasma

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