Sucrose hydrolysis by invertase using a membrane reactor: effect of membrane cut-off on enzyme performance

Addezio, Francesco Di; Yoriyaz, E.J.; Cantarella, Maria; Vítolo, Michele

doi:10.1590/s1984-82502014000200004

Cited by 5 publications

(3 citation statements)

References 3 publications

(4 reference statements)

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“…For example, the hydrolysis of sucrose by invertase is expressed as total-reducing sugars formed per minute, the collagen hydrolysis by collagenase is expressed as the viscosity diminution of a standard collagen solution per minute, and the amylase activity expressed as a SKB unit (10),(11), (12) . However, it is recommended to express the enzyme activity using International Unit (U), which is defined as the amount of enzyme that catalyzes the formation of 1 mole of (13,14) product per minute in fixed conditions. The specific activity is defined as U/mg protein (if the enzyme preparation is impure), or U/mol enzyme (for pure enzymes) (15) .…”

Section: Expression Of the Enzyme Activitymentioning

confidence: 99%

Study the Behavior of Activity and Express for Enzymes

Antesar Rheem Obead,

Anmar Hameed Bloh,

Haider Rasol Abbass Alwahami

2021

Indian Journal of Forensic Medicine & Toxicology

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Enzymes are be defined as soluble, colloidal, organic catalysts which are produced by living cells. Enzymes are biologic catalysts to accelerate the rate of biochemical reactions. in this study Quantification of the Enzyme Activity, Where: E = enzyme concentration; S = substrate concentration; ES = enzyme-substrate complex concentration; k1, k3= 1st order reaction rate constants (t-1); P = product concentration; k2= 2nd reaction rate constant (M-1.t-1).At first, it must be stressed that the formation of ES (enzyme-substrate complex) is an obligatory step to any type of enzyme-catalyzed reaction. The ES, depending on the reaction conditions, can form the product (forward reaction) or not (reward reaction). The immobilization technique allowed a more frequent use of biomaterials in industry, therapeutics (confection of subcutaneous capsules for controlled delivering of hormones), and lab analysis (automatic dosing equipments, enzyme electrode, biosensors and enzyme immunoassays). In industry, the use of glucose isomerase for the conversion of glucose into fructose (high fructose corn syrup), the aminoacylase for the separation of racemic mixture of amino acids, microbial lipase in triglyceride hydrolysis, and the lactase for removing lactose from milk and/or whey are stressed.

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Section: Expression Of the Enzyme Activitymentioning

confidence: 99%

Study the Behavior of Activity and Express for Enzymes

Antesar Rheem Obead,

Anmar Hameed Bloh,

Haider Rasol Abbass Alwahami

2021

Indian Journal of Forensic Medicine & Toxicology

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“…In the food and beverage industries, invertase has been largely employed in the production of invert syrup and high fructose syrup (Addezio et al 2014;Ashraf & Bilal 2015). Invertase is used as an alternative to the traditional acid hydrolysis method which might produce undesirable by-products, unfavourable colour, and bitter taste; could also result in low conversion efficiencies and high ash contents (Aburigal et al 2014).…”

Section: Invertase In the Food And Beverage Industriesmentioning

confidence: 99%

Structural Properties, Production, and Commercialisation of Invertase

Pang¹,

Ramli²,

Johari³

2019

JSM

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The knowledge gained from yeast fermentation made invertase one of the earliest exploited enzymes in human history. Invertase functions as carbohydrases by hydrolysing sucrose into its simplest unit. Extensive studies on invertase have made it well-characterised through the discovery of its existence in a variety of living organisms. It is interesting to study the different types of invertase from either the same or different origins as they might have distinct properties and could possess unique characteristics. With the advancement in technology, the three-dimensional structure, catalytic domain, and mechanism of invertase action have been discovered. Furthermore, it is important to understand how this enzyme has been produced via fermentation or recombinant technology methods. Finally, invertase has been employed in several important industries and its future commercialisation is promising.

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“…However, it has the downside of high cost when compared with the acid process. Nonetheless, the enzymatic process may provide a high degree of hydrolysis when the kinetic criteria of an enzyme are well balanced with the operation, e.g., the use of enzymes immobilized in insoluble solid supports (CABRAL et al, 2017;DI ADDEZIO, 2014). The product yielded is of superior quality, with low contents of ash, color, and HMF.…”

Section: Introductionmentioning

confidence: 99%

Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes

et al. 2022

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The best conditions for in vitro sucrose hydrolysis based on invertase from Candida guilliermondii (ICg) were studied and the kinetic parameters KM,Vmax, and thermal stability of ICg were determined. Candida guilliermondii (Cg) yeast isolated and lyophilized from peach solid wastes was identified using the API 20C AUX method. Subsequently, the Cg was submitted to an autolysis process using NaHCO3 at 200 mM under 200 rpm stirring and 40 °C for 24 h. The enzyme extracts obtained were recovered through precipitation with acetone followed by dialysis and ion-exchange chromatography. The extract purified through precipitation with acetone had activity of 27.7 U.mg-1 and 56% recovery whereas the chromatography process yielded 46.5 U.mg-1 and 44.8%. The optimal sucrose hydrolysis conditions were pH 5.0 and 50 °C, resulting in KM of 30.5 mM and 28.7 mM sucrose, respectively, at 25 °C and 50 °C, both with Michaelian behavior. Thermal inactivation of ICg exhibited first-order apparent kinetics and its residual activity was typically linear between 40 °C and 70 °C. Three isoenzymes were detected through electrophoresis.

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Sucrose hydrolysis by invertase using a membrane reactor: effect of membrane cut-off on enzyme performance

Cited by 5 publications

References 3 publications

Study the Behavior of Activity and Express for Enzymes

Study the Behavior of Activity and Express for Enzymes

Structural Properties, Production, and Commercialisation of Invertase

Extraction, purification and characterization of invertase from Candida guilliermondii isolated from peach solid wastes

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