In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

Abstract

In 1820, French naturalist August Saint Hillaire, during a visit in Espírito Santo (ES), a state in southeastern Brazil, reported a popular use of Cyperaceae species as antidote to snake bites. The plant may even have a hypotensive effect, though it was never properly researched. The in vitro inhibitory of the angiotensin converting enzyme (ACE) activity of eigth ethanolic extracts of Cyperaceae was evaluated by colorimetric assay. Total phenolic and flavonoids were determined using colorimetric assay. The hypotensive effect of the active specie (Rhychonospora exaltata, ERE) and the in vivo ACE assay was measured in vivo using male Wistar Kyoto (ERE, 0.01-100mg/kg), with acetylcholine (ACh) as positive control (5 µg/kg, i.v.). The evaluation of ACE in vivo inhibitory effect was performed comparing the mean arterial pressure before and after ERE (10 mg/kg) in animals which received injection of angiotensin I (ANG I; 0,03, 03 and 300 µg/kg, i.v.). Captopril (30 mg/kg) was used as positive control. Bulbostylis capillaris (86.89 ± 15.20%) and ERE (74.89 ± 11.95%, ERE) were considered active in the in vitro ACE inhibition assay, at 100 µg/mL concentration. ACh lead to a hypotensive effect before and after ERE's curve (-40±5% and -41±3%). ERE showed a dose-dependent hypotensive effect and a in vivo ACE inhibitory effect. Cyperaceae species showed an inhibitory activity of ACE, in vitro, as well as high content of total phenolic and flavonoids. ERE exhibited an inhibitory effect on both in vitro and in vivo ACE. The selection of species used in popular medicine as antidotes, along with the in vitro assay of ACE inhibition, might be a biomonitoring method for the screening of new medicinal plants with hypotensive properties.Uniterms: Cyperaceae/species/pharmacognosy. Cyperaceae/species/in vitro inhibitory activity. Cyperaceae/species/in vivo hypotensive effect. Angiotensin converting enzyme/in vitro inhibitory. Flavonoids/determination. Em 1820, o naturalista francês August Saint Hillaire, durante uma visita ao Espírito Santo (ES), Estado do sudeste do Brasil, relatou o uso popular de espécies de Cyperaceae como antídoto para picadas de cobra. As espécies podem possuir efeito hipotensor, embora nunca tenham sido devidamente pesquisadas. A inibição in vitro da atividade da enzima conversora da angiotensina (ECA) de oito extratos etanólicos de Cyperaceae foi avaliada por ensaio colorimétrico. Totais de fenólicos e flavonóides foram determinados utilizando ensaio colorimétrico. O efeito hipotensor da espécie ativa (Rhychonospora exaltata, ERE) no ensaio de ECA in vitro foi avaliada in vivo utilizando-se machos Wistar Kyoto (ERE, 0.01-100 mg/kg), com a acetilcolina (ACh), como controle positivo (5 µg/kg, iv). A avaliação do efeito inibidor da ECA in vivo foi realizado comparando-se a pressão arterial média, antes e após ERE (10 mg/kg) nos animais que receberam injeção de angiotensina I (Ang I, 0,03, 03 e 300 µg/kg, iv). Captopril (30 mg/kg) foi utilizado como controle positivo. Bulbostylis capillaris (86,89±15,20%) e ERE (7...

Plantas medicinales hipotensoras y sus productos comercializados en el Área Metropolitana de Buenos Aires, Argentina

“…Hipotensor, vasodilatador (Hurrell & Puentes, 2017;Puentes, 2017). (Bastos et al, 2010;Moreira et al, 2010;Martins et al, 2013;García Mesa, 2014). (Puentes, 2017).…”

Efek Vasodilatasi Dan Inhibisi Angiotensin Converting Enzyme Dari Ekstrak Etanol Dan Fraksi Daun Binahong (Anredera Cordifolia (Ten). V. Steenis)

“…Berdasarkan aktivitas inhibisi ACE, suatu senyawa dinyatakan mempunyai aktivitas lemah, sedang, dan kuat jika memiliki persen inhibisi berturutturut 30-50%, >50-80%, dan >80%. Senyawa yang memiliki persen inhibisi <30% pada konsentrasi 100 μg/ml dinyatakan tidak aktif (Martins et al 2013). Dengan demikian, ekstrak etanol daun binahong merupakan inhibitor ACE sedang, fraksi etil asetat dan fraksi air merupakan inhibitor lemah sedangkan fraksi n-heksana tidak memiliki aktivitas inhibisi ACE.…”

Acute Toxicity and Antioxidant and Antibacterial Activities of Kyllinga polyphylla Willd. ex Kunth, Cyperaceae Family

“…Currently, many studies on the biological activity of plants have been carried out, in which plants belonging to the family Cyperaceae have been recorded with about 5,000 species (in 104–122 genera) and have been described [ 12 ]. According to research by Martins et al [ 13 ], many species of the Cyperaceae family have been scientifically demonstrated for biological activities in both in vivo and in vitro studies. However, many species of the genus Kyllinga (there are about 40 species distributed worldwide [ 14 ]) have remained largely untested for their medicinal potential in a scientific manner and therefore require additional research.…”