Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Benetti, Thalyta Marina; Monteiro, Cristina; Beux, Márcia Regina; Abrahão, Wanda Moscalewski

doi:10.1590/s1984-82502012000400011

Cited by 3 publications

(2 citation statements)

References 13 publications

(13 reference statements)

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“…Preliminary identification of LM was done by Gram staining, hemolysis pattern on 5% sheep blood agar, and observation of its tumbling motility in a wet mount light microscopy after overnight incubation of typical colonies inoculated in TSBYE at 25 °C and 37 °C. Furthermore, Catalase, Oxidase, CAMP factor test, and sugars fermentation (mannitol, maltose, dextrose, sucrose, lactose, and rhamnose) was performed for species identification and confirmation of LM [ 14 – 16 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia

et al. 2021

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Background Listeria monocytogenes (LM) has come to be a major public health issue of at-risk groups, causing high morbidity and mortality. Despite this data, studies are very limited in developing countries like Ethiopia. Thus, we aimed to isolate and characterize LM in terms of antibiogram and biofilm formation among pregnant women with fever, women with a history of spontaneous abortion, women with a history of fetal loss, and women with preterm delivery at Jimma University Medical Center (JUMC), southwest Ethiopia. Methods A cross-sectional study was done among 144 women from June to August 2019. Isolates were tested for antibiotic susceptibility and biofilm formation using disc diffusion and microtiter plate method, respectively. Data were collected using a structured questionnaire, entered into Epidata 3.1 and logistic regression was done by SPSS v25.0. Results LM was isolated in 8 (5.56%) of 144 screened women. The isolation rate of LM was relatively higher among women with a history of fetal loss (9.7%), followed by women with preterm delivery (6.25%). One of the six cord blood was positive for LM, indicating that the transplacental transmission rate at JUMC was 16.7%. More than 2% of women with an ongoing pregnancy were found to have LM septicemia, which could hurt their fetus. All of the isolates tested were susceptible to Ampicillin. However, all of the isolates were resistant to Penicillin and Meropenem and were biofilm producers. Conclusions The high magnitude of pregnancy-related listeriosis in the current study setting appears that implementation of educational programs targeting risk reduction and more studies to identify sources of LM are warranted. The choice of antibiotics should be after susceptibility testing.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia

et al. 2021

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“…Different antimicrobials are added to the media to obtain sufficient selectivity. Chromogenic media is the most used culture confirmation method because of its easy preparation and interpretation [20].…”

Section: Introductionmentioning

confidence: 99%

Rapid Monitoring of Listeria monocytogenes in Raw Minced Meat Using Real-Time Polymerase Chain Reaction

Habib¹

2013

IOSR-JESTFT

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Listeria monocytogenes is a pathogenic bacteria of great concern in the food industry due to its wide distribution in the environment, its ability to grow at low temperatures and relatively high salt levels, and the potential severity of the infection with high mortality, mainly in pregnant women, the elderly and immunocompromised patients. Food-borne listeriosis remains a public health threat. One hundred forty two minced raw meat samples were collected from various points (processing plants, hotels, restaurants, retail outlets, caterers and wholesalers) and were analyzed for the detection of L. monocytogenes by conventional culture and Real-time polymerase chain reaction (Real-time PCR). Our results showed frequent contamination of raw minced meats (21%). Our statistical analysis showed a relatively correlation (R 2 = 0.637, p <0.01) between the two methods, while the sensitivity, specificity, and positive and negative PCR method predictive values were respectively 30, 92, 37 and 97%. Continuous environmental monitoring schemes for L. monocytogenes are of major importance to identify potential contamination sources and as an early warning system for meat business operators. Hence, the detection of L. monocytogenes by Real-time PCR method allows good control of the health risk.

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The prevalence of Listeria monocytogenes in meat products in Brazil: A systematic literature review and meta-analysis

Cavalcanti

Limeira

Siqueira

et al. 2022

Research in Veterinary Science

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Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Cited by 3 publications

References 13 publications

Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia

Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia

Rapid Monitoring of Listeria monocytogenes in Raw Minced Meat Using Real-Time Polymerase Chain Reaction

The prevalence of Listeria monocytogenes in meat products in Brazil: A systematic literature review and meta-analysis

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