Mushroom tyrosinase inhibitory activity and major fatty acid constituents of Amazonian native flora oils

Teixeira, Raquel da Silva; Rocha, Paula Rafaela; Polonini, Hudson; Brandão, Marcos Antônio Fernandes; Chaves, Maria das Graças Afonso Miranda; Raposo, Nádia Rezende Barbosa

doi:10.1590/s1984-82502012000300006

Cited by 24 publications

(19 citation statements)

References 8 publications

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“…An additional incubation period of 60 min at 30 ± 1 °C was performed, after which a new spectrophotometric reading was taken (T2). The inhibitory percentages at the two time points (T1 and T2) were obtained according to the formula: 𝐼𝐼𝐼𝐼% = 𝑐𝑐 − 𝑆𝑆 × 𝑐𝑐 100, where IA% = inhibitory activity; C = negative control absorbance; S = sample or positive control absorbance (absorbance at time T1 or T2 minus the absorbance at time T0) [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells

Choi

Yang

et al. 2018

IJMS

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Phyllostachys nigra var. henosis, a domestic bamboo species, has been attracting much attention; its bioactive compounds (especially in the leaf) show antioxidant, anti-inflammatory, and anti-obesity activities. Little information is available on the antioxidative and anti-melanogenetic activities of the bioactive compounds in bamboo stems. The anti-melanogenic and antioxidative activities of the EtOAc fraction (PN3) of a P. nigra stem extract were investigated in a cell-free system and in B16F10 melanoma cells. PN3 consisted of a mixture of flavonoids, such as catechin, chlorogenic acid, caffeic acid, and p-coumaric acid. The antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)), and hydroxyl radical scavenging) was evaluated, as well as the inhibition of reactive oxygen species (ROS) produced by the Fenton reaction. PN3 showed in vitro tyrosinase inhibition activity with the half maximal inbihitory concentration (IC50) values of 240 μg/mL, and in vivo cytotoxic concentration ranges > 100 μg/mL. The protein expression levels and mRNA transcription levels of TYR, TRP-1, and MITF were decreased in a dose-dependent manner by the treatment with PN3. PN3 interfered with the phosphorylation of intracellular protein kinase A (PKA)/cAMP response element-binding protein (CREB), demonstrating potent anti-melanogenic effects. PN3 could inhibit PKA/CREB and the subsequent degradation of microphthalmia-associated transcription factor (MITF), resulting in the suppression of melanogenic enzymes and melanin production, probably because of the presence of flavonoid compounds. These properties make it a candidate as an additive to whitening cosmetics.

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Section: Methodsmentioning

confidence: 99%

Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells

Choi

Yang

et al. 2018

IJMS

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“…Plant seed oils continue to receive growing interest from researchers working in a variety of different fields, including chemists, pharmacists, and physicians because of their potential applications in the pharmaceutical, cosmetics, and food industries [ 26 ]. Torreya grandis cv.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Tyrosinase Inhibitory Activities of Seed Oils from Torreya grandis Fort. ex Lindl.

Cui

Duan

Jia

et al. 2018

BioMed Research International

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Torreya grandis Fort. ex Lindl. is a plant belonging to the Taxaceae family and Torreya grandis cv. Merrillii is the only grafted and thoroughbred species belonging to this species. In this study, we extracted five different seed oils, including T. grandis seed oil (TGSO), T. grandis “Xiangyafei” seed oil (XYSO), T. grandis “Zhimafei” seed oil (ZMSO), T. grandis “Majus”seed oil (TGMSO), and T. grandis “cunguangfei” seed oil (CGSO) using physical pressure. The resulting extracts were analyzed to determine their fatty acid composition, antioxidant activity, and inhibitory activity towards tyrosinase. The results of the antioxidant activity assays revealed that XYSO and ZMSO exhibited much greater DPPH radical scavenging activity and ferric reducing power than TGSO. Notably, all five of the seed oils showed dose-dependent inhibitory activity towards tyrosinase. XYSO and TGSO gave the highest activities of all of the seed oils tested in the current study against monophenolase and diphenolase, with IC50 values of 227.0 and 817.5μg/mL, respectively. The results of this study show that wild TGSOs exhibit strong antioxidant and tyrosinase inhibition activities. These results therefore suggest that wild TGSOs could be used as a potential source of natural antioxidant agents and tyrosinase inhibitors.

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“…Tyrosinase inhibitory activity was carried out in accordance with Teixera et al (2012) with slight modifications. 25 μL of sample was reacted with 80 μL phosphate buffer, 125 μL L-tyrosine and 20 μL tyrosinase enzymes, and then incubated for 30 min at room temperature (Teixeira et al 2012). The absorbance was then recorded using a Multiscan (Nanoquant, Infinite M2000 Pro, TECAN) at 475 nm.…”

Section: Tyrosinase Inhibitory Activitymentioning

confidence: 99%

Transformation of Melinjo Seed Micropowders into Nanopowders Enhances Extractability of Phenolic Compounds and Tyrosinase Inhibitory Activity

Saraswaty¹,

Suparta²,

Setiyanto³

et al. 2019

JSM

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Melinjo (Gnetum gnemon L.) seed powder extract exhibited antioxidant and tyrosinase inhibitory activity, thus showing potential as a dietary supplement or nutraceutical to prevent aging or hyperpigmentation. Previously, we found that particle size plays important role in extraction of bioactive compounds and influences their bioactivity. Thus, it is important to determine a certain particle size for the extraction process. In this study, we investigated the effects of transformation of melinjo seed micropowders into nanopowders by nanomilling using a high-energy ball mill (shaker mill). The effects of melinjo seed powders particle size on its physicochemical characteristics, extraction efficiency and release of phenolic compounds, as well as tyrosinase inhibitory activity were observed. The nanomilling successfully transformed melinjo seed micropowders into nanopowders within 90 min of milling. Particle size analysis showed that melinjo seed nanopowders were produced with a mean particle diameter of ~675 nm (PI 0.270). Scanning electron microscope (SEM) images of the melinjo seed nanopowders obviously showed a smaller particle size, a smooth surface, amorphous shapes and irregular edges. The melinjo seed nanopowders at mean particle diameter of ~675 nm exhibited the highest extraction yield and phenolic compounds release. As a consequence, the tyrosinase inhibitory activity of the melinjo seed nanopowders was 4.5 times higher than that of the melinjo seed micropowders. Based on the results obtained in this study, transformation of melinjo seed micropowders into nanopowders is very promising for improving the efficacy of melinjo seed as tyrosinase inhibitor.

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Mushroom tyrosinase inhibitory activity and major fatty acid constituents of Amazonian native flora oils

Cited by 24 publications

References 8 publications

Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells

Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells

Antioxidant and Tyrosinase Inhibitory Activities of Seed Oils from Torreya grandis Fort. ex Lindl.

Transformation of Melinjo Seed Micropowders into Nanopowders Enhances Extractability of Phenolic Compounds and Tyrosinase Inhibitory Activity

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