Evaluation of anti-estrogenic or estrogenic activities of aqueous root extracts of Gunnera manicata L.

Mariotti, Kristiane de Cássia; Schmitt, Gabriela Cristina; Barreto, Fabiano; Fortunato, Rodrigo E.; Singer, Rodrigo B.; Dallegrave, Eliane; Leal, Mirna Bainy; Limberger, Renata Pereira

doi:10.1590/s1984-82502011000300018

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…Overall, the partial agonist effect of the ethyl acetate extract might be produced by the interaction of the compounds. The compounds might work individually, synergistically, or antagonistically or as described in other studies [79,80]. To confirm that the mechanism of action of the compounds occurs via the classical ligand-dependent mechanism, the binding capability of compounds to ERs was characterized using molecular docking.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Phenylpropanoid and Lignan Compounds from Peperomia pellucida [L.] Kunth with Estrogenic Activities

et al. 2020

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Extracts of Peperomia pellucida [L.] Kunth have previously been demonstrated to have in vivo estrogenic-like effects, thereby functioning as an anti-osteoporotic agent. However, the compounds responsible for these effects have not yet been determined. Therefore, the aim of this study is to isolate and elucidate potential compounds with estrogenic activity. The structures of the isolated compounds were identified using 1D 1H and 13C-NMR and confirmed by 2D FT-NMR. The estrogenic activity was evaluated using the E-SCREEN assay, and a molecular docking study was performed to predict the binding affinity of the isolated compounds to estrogen receptors. In this experiment, we successfully isolated three phenylpropanoids and two lignan derivatives, namely, 6-allyl-5-methoxy-1,3-benzodioxol-4-ol (1), pachypostaudin B (2), pellucidin A (3), dillapiole (4), and apiol (5). Among these compounds, the isolation of 1 and 2 from P. pellucida is reported for the first time in this study. Activity assays clearly showed that the ethyl acetate extract and its fractions, subfractions, and isolated compounds exerted estrogenic activity. Methanol fraction of the ethyl acetate extract produced the highest estrogenic activity, while 1 and 2 had partial agonist activity. Some compounds (derivates of dillapiole and pellucidin A) also had, in addition, anti-estrogenic activity. In the docking study, the estrogenic activities of 1–5 appeared to be mediated by a classical ligand-dependent mechanism as suggested by the binding interaction between the compounds and estrogen receptors; binding occurred on Arg 394 and His 524 of the alpha receptor and Arg 346 and His 475 of the beta receptor. In summary, we reveal that P. pellucida is a promising anti-osteoporotic agent due to its estrogenic activity, and the compounds responsible for this activity were found to be lignan and phenylpropanoid derivatives. The presence of other compounds in either the extract or fraction may contribute to a synergistic effect, as suggested by the higher estrogenic activity of the methanol fraction. Hence, we suggest further research on the osteoporotic activity and safety of the identified compounds, especially regarding their effects on estrogen-responsive organs.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Phenylpropanoid and Lignan Compounds from Peperomia pellucida [L.] Kunth with Estrogenic Activities

et al. 2020

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“…The Gunnera manicata L. species is a native plant from Southern Brazil, it grows at high altitudes (above 900m) on the border between the states of Rio Grande do Sul and Santa Catarina, in a region locally known as "Aparados da Serra". Despite being appreciated as an ornamental plant and belonging to the same family (Gunneraceae) as G. perpensa (an extensively studied species), little data is found on G. manicata in scientific literature, and this includes the absence of anti-estrogenic activity (Mariotti et al, 2011a) and the acute toxicity of aqueous extracts of G. manicata against Wistar rats, that showed a high margin of safety (Mariotti et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

Chemical constituents and pharmacological profile of Gunnera manicata L. extracts

Mariotti

Schuh

Nunes

et al. 2014

Braz. J. Pharm. Sci.

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Gunnera perpensa L. (Gunneraceae) is a native South African plant widely used in traditional medicine as an antibacterial and antifungal. In southern Brazil there is the native species called Gunnera manicata L. that also belongs to the Gunneraceae. Nevertheless, there is no information about chemical and pharmacological properties of South American Gunnera species. Therefore this study aimed at assessing the phytochemical and pharmacological profiles of aqueous and methanol Brazilian G. manicata extracts. The results showed that antimicrobial activity in an agar diffusion assay was effective against Staphylococcus aureus and Candida albicans . Phenolic compounds were investigated by liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS) and all extracts presented gallic acid and only the methanol extract obtained from the leaves exhibited hyperoside. Rutin, quercetin and chlorogenic acid were not found in the samples analysed. Total phenolic content was higher in methanol extract and total flavonoid content was low in all extracts. Antioxidant activity was evaluated by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical test, and all samples presented good to moderate antioxidant activity. These results encourage complementary studies on the chemical composition of the plant extracts focusing on isolation and structure elucidation of their active compounds.

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From Traditional Usage to Pharmacological Evidence: Systematic Review ofGunnera perpensaL.

Maroyi

2016

Evidence-Based Complementary and Alternative Medicine

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Gunnera perpensa is the only species of the genus Gunnera that has been recorded in Africa. Its leaves, rhizomes, roots, and stems are reported to possess diverse medicinal properties and used to treat or manage various human and animal diseases and ailments. Gunnera perpensa is an ingredient in many herbal concoctions and prescriptions which have been used to induce or augment labour, postnatal medication, to treat parasitic diseases, urinary complaints, kidney problems, general body pains, sexually transmitted infections, and many other diseases. Several classes of phytochemicals including alkaloids, benzoquinones, ellagic acids, flavonoids, phenols, proanthocyanidins, tannins, and minerals have been isolated from G. perpensa. Scientific studies on G. perpensa indicate that it has a wide range of pharmacological activities including acetylcholinesterase, anthelmintic, antibacterial, antifungal, antinociceptive, anti-inflammatory, antioxidant, antitumour, lactogenic, and uterotonic. Gunnera perpensa has a lot of potential as a possible source of pharmaceutical products for the treatment of a wide range of both human and animal diseases and ailments. Some of the chemical compounds isolated from G. perpensa have demonstrated various biological activities when investigated in in vitro assays. Future research should focus on the mechanisms of action of the isolated compounds, their efficacy, toxicity, and clinical relevance.

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Evaluation of anti-estrogenic or estrogenic activities of aqueous root extracts of Gunnera manicata L.

Cited by 4 publications

References 7 publications

Isolation and Characterization of Phenylpropanoid and Lignan Compounds from Peperomia pellucida [L.] Kunth with Estrogenic Activities

Isolation and Characterization of Phenylpropanoid and Lignan Compounds from Peperomia pellucida [L.] Kunth with Estrogenic Activities

Chemical constituents and pharmacological profile of Gunnera manicata L. extracts

From Traditional Usage to Pharmacological Evidence: Systematic Review ofGunnera perpensaL.

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