Development of methodologies for dimethylaminoethanol glycolate assay in association with sunscreens in dermocosmetic formulation

Deccache, Daniela Soares; Santos, Elisabete Pereira dos; Cabral, Lúcio Mendes; Rodrigues, Carlos Rangel; Sousa, Valéria Pereira de

doi:10.1590/s1984-82502010000400012

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…Similar results were observed in a previous study of centrifugation at 6000 rpm for 15 min (Deccache et al, 2010). Although conductimetric tracer leaked from some of the samples kept at higher temperatures, the results of centrifugation indicate that emulsions were stable enough against any phase separation.…”

Section: Centrifugation and Phase Separationsupporting

confidence: 89%

Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract

Mahmood

Akhtar

Khan

et al. 2013

Braz. J. Pharm. Sci.

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Complex multiple emulsions have an excellent ability to fill large volumes of functional cosmetic agents. This study was aimed to encapsulate large volume of green tea in classical multiple emulsion and to compare its stability with a multiple emulsion without green tea extract. Multiple emulsions were developed using Cetyl dimethicone copolyol as lipophilic emulsifier and classic polysorbate-80 as hydrophilic emulsifier. Multiple emulsions were evaluated for various physicochemical aspects like conductivity, pH, microscopic analysis, rheology and these characteristics were followed for a period of 30 days in different storage conditions. In vitro and in vivo skin protection tests were also performed for both kinds of multiple emulsions i.e. with active (MeA) and without active (MeB). Both formulations showed comparable characteristics regarding various physicochemical characteristics in different storage conditions. Rheological analysis showed that formulations showed pseudo plastic behavior upon continuous shear stress. Results of in vitro and in vivo skin protection data have revealed that the active formulation has comparable skin protection effects to that of control formulation. It was presumed that stable multiple emulsions could be a promising choice for topical application of green tea but multiple emulsions presented in this study need improvement in the formula, concluded on the basis of pH, conductivity and apparent viscosity data. Uniterms:Cosmetics. Green tea. Multiple emulsion. Rheology. Skin. Stability.Emulsões múltiplas complexas possuem excelente habilidade de agregar grandes quantidades de agentes cosméticos funcionais. Este estudo teve por objetivo encapsular grandes volumes de chá verde em uma emulsão múltipla clássica e comparar sua estabilidade com a emulsão múltipla sem o extrato do chá verde. Emulsões múltiplas são desenvolvidas usando cetil dimeticona copoliol como emulsificante lipofílico e o clássico polissorbato-80 como emulsificante hidrofílico. As emulsões múltiplas foram avaliadas por meio de vários aspectos fisico-químicos como condutividade, pH, análise microscópica e reologia. Estas características foram observadas por um período de 30 dias sob diferentes condições de armazenamento. Testes de proteção da pele in vivo e in vitro foram realizados para ambos os tipos de emulsões testadas, i.e. com o ativo em estudo (MeA) e sem ativo (MeB). Ambas as formulações apresentaram características comparáveis no que diz respeito aos diferentes fatores físico-químicos avaliados sob diferentes condições de armazenamento. A análise reológica mostrou que as formulações apresentaram comportamento pseudoplástico sob contínuo estresse de cisalhamento. Os resultados dos testes in vivo e in vitro sobre a proteção da pele revelaram que a formulação ativa promoveu efeitos comparáveis à formulação controle. Nossos dados mostraram que emulsões múltiplas estáveis poderiam ser escolhas promissoras para a aplicação tópica do chá verde. Entretanto, a fórmula das emulsões múltiplas apresentadas neste estudo...

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Section: Centrifugation and Phase Separationsupporting

confidence: 89%

Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract

Mahmood

Akhtar

Khan

et al. 2013

Braz. J. Pharm. Sci.

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“…SPF cream formulated with the EF of P. boergesenii brown seaweed in this study reported 20.55. Many studies have reported on the determination of SPF in sunscreens in various forms (creams, lotions, and gels) [ 75 , 76 , 77 ]. Although adding more brown algae fraction to a cream formulation may increase its protective properties, doing so could create an unstable emulsion.…”

Section: Discussionmentioning

confidence: 99%

Potential of the Ethyl Acetate Fraction of Padina boergesenii as a Natural UV Filter in Sunscreen Cream Formulation

Soleimani

Yousefzadi

Nezhad

et al. 2023

Life

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Brown seaweeds, due to their wide range of bioactive compounds, have a high ability to inhibit free radicals and protect against ultraviolet rays. In the present study, the ethyl acetate fraction (EF) was isolated from the Padina boergesenii brown seaweed. Antioxidant activity (by the DPPH scavenging activity method) and cytotoxicity against UVB-induced cytotoxicity in HaCaT human keratinocytes were evaluated. Then, this fraction was used as a bio-filter in the formulation of sunscreen, and the physical properties and stability were investigated. The results showed that the EF could inhibit DPPH radical scavenging (54 ± 1%) and cell viability of HaCaT keratinocytes exposed to UVB irradiation (81.2 ± 0.1%). The results of the stability study of the cream formulated with EF showed that at temperatures 4 °C and 25 °C it has high stability; and at 40 °C on the 28th day, a slight decrease in its stability was observed. The pH and Sun Protection Factor of the cream formulated with EF were reported at 5.8 and 20.55, respectively. Also, the DPPH scavenging activity of the cream was not altered for 28 days of storage at temperatures of 4–40 °C. According to our results, it was proved that the sunscreen formulated with EF of P. boergesenii brown seaweed has promising properties and characteristics that can create a new opportunity for the development of cosmetics and skin care products.

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“…SPF cream formulated with the ethyl acetate fraction of the brown alga P. boergesenii in this study reported 20.55. Many studies have been reported on the determination of SPF in sunscreens in various forms (creams, lotions, and gels)(Deccache et al 2010;Mota et al 2019;Pawar et al 2015)…”

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confidence: 99%

Utilization of the ethyl acetate fraction of Padina boergesenii as a natural UV filter in sunscreen cream formulation

Soleimani

Yousefzadi

Nezhad

et al. 2022

Preprint

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Brown seaweeds due to their wide range of bioactive compounds have a high ability to inhibit free radicals and protect against ultraviolet rays. In the present study, the ethyl acetate fraction was isolated from the Padina boergesenii brown alga. Antioxidant activity (by inhibition DPPH free radical method) and cytotoxicity against UVB-induced cytotoxicity in HaCaT human keratinocytes were evaluated. Then, this fraction was used as a biofilter in the formulation of sunscreen and was investigated physical properties and stability. The results showed that the ethyl acetate fraction could inhibit DPPH radical scavenging (54 ±1 %) and the ability to cell viability of HaCaT keratinocytes exposed to UVB irradiation (81.2 ± 0.1 %). The results of the stability study of the cream formulated with ethyl acetate fraction showed that temperatures 4°C and 25°C have high stability and at 40°C on the 28th day, was observed a slight decrease in its stability. The pH and SPF of the cream formulated with ethyl acetate fraction were reported at 5.8 and 20.55, respectively. Also, the DPPH scavenging activity of the cream was not altered for 28 days of storage at temperatures of 4 - 40°C. According to the obtained results, it was proved that the sunscreen formulated with ethyl acetate fraction of Padina boergesenii brown seaweed has promising properties and characteristics that can create a new opportunity for the development of cosmetics and skin care products.

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Development of methodologies for dimethylaminoethanol glycolate assay in association with sunscreens in dermocosmetic formulation

Cited by 6 publications

References 13 publications

Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract

Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract

Potential of the Ethyl Acetate Fraction of Padina boergesenii as a Natural UV Filter in Sunscreen Cream Formulation

Utilization of the ethyl acetate fraction of Padina boergesenii as a natural UV filter in sunscreen cream formulation

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