Comparative in vitro antioxidant potential of different parts of Ipomoea asarifolia, Roemer & Schultes, Guiera senegalensis, J. F. Gmel and Anisopus mannii N. E. Brown

Abstract

In this study, the radical scavenging capacity of Guiera senegalensis, Ipomoea asarifolia and Anisopus mannii were compared. The ascorbic acid equivalent of the stem bark (2.1 mM), leaves (2.05 mM) and root barks (1.89 mM) of Guiera senegalesis are much higher than any part of Ipomoea asarifolia or Anisopus manii. The stem bark, leaves and root bark of Guiera Senegalensis had IC 50 of 15.4, 20.0 and 17.0 µL respectively, while corresponding parts of Ipomoea asarifolia showed IC 50 of 50, 42 and 65 µL, respectively. These results suggest that Guiera senegalensis and Ipomoea asarifolia possess significant antioxidant capacities to warrant further detailed studies on the role of this property in their therapeutic effects. Uniterms INTRODUCTIONPlants continue to be a major source of medicine, as they have always been throughout human history (Ampofo, 1977;Farnsworth et al., 1985;Eisenberg et al., 1998). A number of these plants from other continents have been reported to exert therapeutic efficacies through their antioxidant activities (Aruoma, 2003;Park, Pezzuto, 2002;Scartezzini, Speroni, 2000;Zhu et al., 2004) Ipomoea asarifolia, Roemer & J.A. Schultes (synonyms: Ipomoea hederifolia) or Morning glory, known in the Hausa language of Nigeria as "Duman Karda" (Kaduna), "Duman ra'afii" (Kano) and "Woba boje" or "Daadi mayo" (Adamawa), is a hairless, succulent perennial weed of the family Convolvulaceae that grows in hydromorphic soils in low lying and inland valleys, streams, and river banks of temperate and tropical countries (Okozie, Agyakwa, 1998). Guiera Senegalensis, J.F. Gmel (Family: Combretaceae), known in Hausa as "Sabera" is a small S. Ene-OjoAtawodi, G. S. Onaolapo 246 shrub with opposite or sub-opposite leaves that are characterized with scattered black glands, which is widespread in West Africa ((Hutchinson et al., 1954), while Anisopus mannii, N.E. Brown (Synonyms: Anisopus bicoronatus, Anisopus rostriferus) is a glabrous twining shrub, known locally as "Kafi-suga" or "Nanukuna", which is a selfsupporting or climbing epiphyte belonging to the family Asclepiadacecae. This plant may possess succulent or non-succulent anticlockwise twines.Our survey revealed that Guiera senegalensis, J.F. Gmel, Ipomoea asarifolia and Anisopus mannii, N.E. Brown are used either individually or in combination with other herbs, for the treatment of gastrointestinal disorders and/or diabetes, respectively, in Northern Nigeria. These diseases are known to have biological oxidation as a major etiological factor (Dandona, 1996; Boynes,1991;Ames et al., 1995;Collier et al., 1990; Cerruti, 1994;Atawodi, 2005). However, there is little or no information in the literature on the mechanism(s) by which Guiera senegalensis, Ipomoea asarifolia and Anisopus mannii exert their therapeutic effects. Therefore, it was considered necessary to evaluate their comparative antioxidant potential to ascertain if this property is one of the underlying bases for their usefulness in folk medicine. MATERIALS AND METHODS Plant collection and identifica...

Phytochemical Investigation of the Roots of Ipomoea asarifolia and Antiproliferative Activity of the Isolated Compounds against Multiple Myeloma Cells

“…is a herbaceous perennial plant growing in South and Central America, as well as in West Africa and South Asia. , The aerial parts have been well studied because of the acute toxicity in cattle due to the presence of ergot alkaloids. ,− Despite its toxicity, the plant is still used in traditional medicine, particularly the roots, against dracunculiasis, postpartum bleeding, and stomachache . The roots have shown antioxidant and diuretic activities, − but no phytochemical investigation has been reported so far.…”

Anti-oxidative and anti-ulcerogenic activity of Ipomoea imperati

“…As already well documented, angiogenesis, the formation of new blood vessels from pre-existing microvasculature, plays an important role in a variety of physiological and pathological states such as embryonic development, wound repair, inflammatory disorders and tumor growth (You et al, 2003). Medicinal plants are showing promise for preventive intervention in the pathogenesis of many diseases, as well as in their treatment, especially the ROS-mediated diseases such as cancer, ulcer and others (Ene-Ojoatowodi & Onaolapo, 2010). The same authors related that in gastrointestinal disorders such as ulcer, the strong antioxidant potential of Ipomoea asarifolia suggest that it is plausible it may exert therapeutic efficacy in oxidative stress related diseases.…”