Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions

Abstract

A simple, rapid, and sensitive fluorimetric method was developed and validated to quantify curcumin in lipid and polymeric nanocapsule suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 nm and 508 nm, respectively. The calibration graph was linear from 0.1 to 0.6 mg/mL with a correlation coefficient of 0.9982. The detection and quantitation limits were 0.03 and 0.10 mg/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.Uniterms: Curcumin/determination. Fluorimetric method/quantitative analysis. Polymeric nanocapsules/ evaluation. Lipid nanocapsules/evaluation.Um método fluorimétrico simples, rápido e sensível foi desenvolvido e validado para quantificação da curcumina em suspensões de nanocápsulas lipídicas e poliméricas, usando acetonitrila como solvente. Os comprimentos de onda de excitação e emissão foram 397 nm e 508 nm, respectivamente. Nas condições testadas, a curva de calibração demonstrou-se linear na faixa de 0,1 a 0,6 mg/mL, exibindo coeficiente de correlação de 0,9982. Os limites de detecção e quantificação foram 0,03 e 0,10 mg/mL, respectivamente. Os resultados da validação confirmaram que o método desenvolvido é específico, linear, exato e preciso para o uso proposto. O presente método foi aplicado com sucesso para a avaliação do teor de curcumina nas suspensões de nanocápsulas lipídicas e poliméricas durante o estágio inicial do desenvolvimento da formulação.Unitermos: Curcumina/determinação. Método fluorimétrico/análise quantitativa. Nanocápsulas poliméricas/avaliação. Nanocápsulas lipídicas/avaliação.

Insecticidal activity of polycaprolactone nanocapsules loaded with Rosmarinus officinalis essential oil in Tribolium castaneum (Herbst)

“…The amount of encapsulated rosemary EO was determined using the filtration–centrifugation technique according to Mazzarino et al () with some modifications. To separate unentrapped EO from nanocapsules, the colloidal suspension was placed in the upper chamber of Amicon centrifugal filter (10 kDa, Millipore, Billerica, MA, USA) and was centrifuged at 4°C and 5000 rpm for 40 min.…”

Relationship between the process variables and physicochemical features of liquid-core nanocapsules produced via nanoprecipitation

“…These results appear reasonable since this drug is highly hydrophobic (water solubility: 120 mg/L at 25°C, class II in the biopharmaceutics classification system) [25]. The EE increases as the drug increase its lipophilicity (becomes non-polar) [60][61][62][63][64]. Figure 3 shows that the EE is independent of the process variables.…”

Utilization of Eosin Dye as an Ion Pairing Agent for Determination of Pharmaceuticals: A Brief Review

“…As revealed above eosin Y is one of the important fluorescent ion pairing agent and spectrofluorimetric technique play an important role in the analysis of many pharmaceutical compounds and active current research area as a number of research articles and reviews published every year on this topic [102][103][104][105][106][107][108]. Spectrofluorimetry provides an extremely sensitive, selective and simple method for a variety of active pharmaceutical ingredients at a very low detection limits up to pictogram range [109].…”