Topical antiinflammatory activity and chemical composition of the epicuticular wax from the leaves of Eugenia beaurepaireana (Myrtaceae)

Abstract

In order to verify the topical antiinflammatory effect of epicuticular wax from leaves of Eugenia beaurepaireana, it was tested in mice croton oil-induced inflammation. Our findings show that topical application of Eugenia beaurepaireana epicuticular wax was significantly active in inhibiting both oedema (Inhibitory dose 50 % (I D 50 ) = 0.31 (0.26 -0.39) mg.ear -1 , inhibition = 79 ± 6 %) and tissue myeloperoxidase activity (indicative of polymorphonuclear leukocytes influx) (I D 50 =0.34 (0.20 -0.41) mg.ear -1 , inhibition = 77 ± 4 %) in mice ear treated with croton oil. Two main compounds were detected on epicuticular wax of E. beaurepaireana. These compounds were identified as α-and β-amyrin by flame ionization detection (GC-FID) and spectroscopic methods (IR, NMR 1 H and 13 C). In conclusion, the results indicate a topical antiinflammatory activity for the Eugenia specie studied and, that, at least in part, α-and β-amyrin are responsible for this activity.Uniterms: Eugenia beaurepaireana/pharmacognosy. α-amyrin. β-amyrin. Epicuticular wax/ antiinflammatory activity. Ear oedema/experimental study.A atividade antiinflamatória tópica da cera epicuticular das folhas de Eugenia beaurepaireana foi avaliada pelo modelo do edema de orelha induzido pelo óleo de cróton em camundongos. Os resultados do estudo mostram que a aplicação tópica da cera epicuticular de Eugenia beaurepaireana inibiu significativamente a formação do edema (Dose inibitória 50 % (DI 50 ) = 0,31 (0,26 -0,39) mg.orelha -1 , inibição = 79 ± 6 %) e a atividade da mieloperoxidase tissular (indicativo do influxo de leucócitos polimorfonucleares) (DI 50 =0,34 (0,20 -0,41) mg.orelha -1 , inibição = 77 ± 4 %) em camundongos tratados com o óleo de cróton. Dois compostos majoritários foram detectados e isolados da cera epicuticular de E. beaurepaireana. Estes compostos foram identificados como os triterpenos α-amirina e β-amirina, através de técnicas cromatográficas (CG-FID) e espectroscópicas (IV, RMN 1 H e 13 C). Em conclusão, os resultados indicam que a espécie E. beaurepaireana apresenta um efeito antiinflamatório tópico relevante, sendo os compostos α-amirina e β-amirina responsáveis, pelo menos em parte, por esta atividade.Unitermos: Eugenia beaurepaireana/farmacognosia. α-amirina. β-amirina. Cera epicuticular/atividade antiinflamatória. Edema de orelha/estudo experimental.

Chemical Composition and In Vitro Antimicrobial Activity of the Essential Oil Obtained from Eugenia pyriformis Cambess. (Myrtaceae)

“…The essential oil of E. pyriformis showed moderate antibacterial potential against the gram-positive bacteria S. aureus and methicillin-resistant S. aureus (MRSA) with MIC values of 250 and 125 μg.mL -1 , respectively. A previous study using the essential oil obtained from E. umbelliflora and E. brasiliensis showed an MIC of 119 and 156 μg.mL -1 , respectively, against S. aureus [24]. The essential oil from E. axillaris had an MIC of 625 µg.mL -1 for S. aureus.…”

“…β-Caryophyllene and bicyclogermacrene are present in moderate amounts in the essential oil obtained from E. burkartiana [19], E. ramboi [19], E. repanda [22], E. cuprea, E. pitanga [23], E. beaurepaireana [23], E. hyemalis [23], E. mattosii [24] and E. klotzschiana [18,25].…”

Morpho-anatomy of the leaf and stem of Eugenia pyriformis

“…Studies on E. beaurepairiana (Kiaersk.) D. Legrand demonstrated that the leaf of this species has anti-infl ammatory properties when topically administered (Magina et al, 2009). In a revision by Auricchio & Bacchi (2003) on E. unifl ora L. (Brazilian cherry), different investigations confi rmed the analgesic, anti-infl ammatory, anti-hypertensive and anti-diabetic effects of this species.…”

Essentials Oils from Brazilian Eugenia and Syzygium Species and Their Biological Activities

“…The specimen from Santa Catarina (Brazil southern) was rich in viridiflorol (17.7%), followed by β- (13.2%) and α-pinene (11.2%) [ 29 ]. E. beaurepairiana oils from Santa Catarina state (Brazil southern) showed high amounts of cyclic sesquiterpene hydrocarbons with germacrane/caryophyllane/cadinane skeleton such as bicyclogermacrene (7.2–14.3%), germacrene D (1.6–8.6%), ( E )-caryophyllene (6.4–8.0%), and δ-cadinene (4.9–7.2%) [ 30 , 31 ].…”