Evolution of erythrocyte morphology in amphibians (Amphibia: Anura)

Wei, Jie; Li, Yanyan; Li, Wei; Ding, Guohua; Fan, Xiaoli; Zhen-shan, Lin

doi:10.1590/s1984-46702015000500005

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…As is well known in 2D microscopy 3 , frog erythrocytes exhibit distinct morphological parameters that are unlike human or mouse erythrocytes. The mean values of the cellular volumes of the frog erythrocytes were 1737.46 ± 299.32 fL, which was two orders of magnitude larger than that of human (90.5 ± 11.4 fL) and of mouse erythrocytes (45.6 ± 6.3 fL).…”

Section: Resultsmentioning

confidence: 87%

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Measurements of three-dimensional refractive index tomography and membrane deformability of live erythrocytes from Pelophylax nigromaculatus

Kim

Lee

Youn

et al. 2018

Sci Rep

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Unlike mammalian erythrocytes, amphibian erythrocytes have distinct morphological features including large cell sizes and the presence of nuclei. The sizes of the cytoplasm and nuclei of erythrocytes vary significantly over different species, their environments, or pathophysiology, which makes hematological studies important for investigating amphibian species. Here, we present a label-free three-dimensional optical quantification of individual amphibian erythrocytes from frogs Pelophylax nigromaculatus (Rana nigromaculata). Using optical diffraction tomography, we measured three-dimensional refractive index (RI) tomograms of the cells, which clearly distinguished the cytoplasm and nuclei of the erythrocytes. From the measured RI tomograms, we extracted the relevant biochemical parameters of the cells, including hemoglobin contents and hemoglobin concentrations. Furthermore, we measured dynamic membrane fluctuations and investigated the mechanical properties of the cell membrane. From the statistical and correlative analysis of these retrieved parameters, we investigated interspecific differences between frogs and previously studied mammals.

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Section: Resultsmentioning

confidence: 87%

“…Because of their distinct morphologies, amphibian erythrocytes have been extensively investigated. Previous studies have suggested that the adaptive structures of the cells are dependent on both intrinsic factors including ages and species 2 , 3 , and extrinsic factors including habitat conditions 4 .…”

Section: Introductionmentioning

confidence: 99%

Measurements of three-dimensional refractive index tomography and membrane deformability of live erythrocytes from Pelophylax nigromaculatus

Kim

Lee

Youn

et al. 2018

Sci Rep

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“…2007; Gregory 2019) reporting cell volume and nucleus volume (both μm 3 ) for red blood cells (erythrocyte) among species of fish (N = 43), birds (N = 105), reptiles (N = 38), and amphibians (N = 73, after complementing with Wei et al . 2015). We also included data for the nucleoid – the analogue of the nucleus in bacteria (N = 37) – that were sourced from Gray et al .…”

Section: Methodsmentioning

confidence: 99%

Larger cells have relatively smaller nuclei across the Tree of Life

Malerba

Marshall

2021

Evolution Letters

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Larger cells have larger nuclei, but the precise relationship between cell size and nucleus size remains unclear, and the evolutionary forces that shape this relationship are debated. We compiled data for almost 900 species – from yeast to mammals – at three scales of biological organisation: among‐species, within‐species, and among‐lineages of a species that was artificially selected for cell size. At all scales, we showed that the ratio of nucleus size to cell size (the ‘N: C’ ratio) decreased systematically in larger cells. Size evolution appears more constrained in nuclei than cells: cell size spans across six orders of magnitude, whereas nucleus size varies by only three. The next important challenge is to determine the drivers of this apparently ubiquitous relationship in N:C ratios across such a diverse array of organisms.

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“…But, if data are collected for different species as well as within species under different conditions some normal ranges of values can be arrived at, which can form a valuable diagnostic aid. Studies of blood parameters had proven to be a valuable approach for analyzing the health status (Wei et al, 2015) of anurans and help in understanding the relationship of blood characteristics to the habitat and adaptability of the species to the environment. The ranges of normal values of the key biochemical parameters are still undefined for same type of animal living in different habitats.…”

Section: Discussionmentioning

confidence: 99%

Hematological Profile of Indian Bullfrog and Common Toad from Different Environmental Habitats

Monalisa¹,

Sonali²,

Kar³

et al. 2021

IJZI

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The wellbeing of human population depends on the ecosystem of earth. Amphibians from the ancient time represents a major part in the global diversity and play important role for the benefit of society worldwide. The aim of the present study was to investigate the comparative hematological study of Indian bullfrog (Hoplobatrachus tigerinus) from wetland and a common Indian toad (Duttaphrynus melanostictus) from terrestrial environment. In this study we have procured 10 sexually mature and disease-free Indian bullfrog (9.93±0.07 cm total length, 59.5±1.94 g weight) and 10 common Indian toad (8.26±0.26 cm total length, 78.6±1.36 g weight). The RBC and WBC count were found to be increased in frog than toad. Variation in RBC morphology which reveals the anemic condition in frog than that of toad. The study suggested that the environmental conditions have significant impact on status of frog and toad.

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Evolution of erythrocyte morphology in amphibians (Amphibia: Anura)

Cited by 7 publications

References 16 publications

Measurements of three-dimensional refractive index tomography and membrane deformability of live erythrocytes from Pelophylax nigromaculatus

Measurements of three-dimensional refractive index tomography and membrane deformability of live erythrocytes from Pelophylax nigromaculatus

Larger cells have relatively smaller nuclei across the Tree of Life

Hematological Profile of Indian Bullfrog and Common Toad from Different Environmental Habitats

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