Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Aguirre-Obando, Oscar Alexander; Bona, Ana Caroline Dalla; Duque, Jonny E.; Navarro-Silva, Mário Antônio

doi:10.1590/s1984-46702015000100003

Cited by 42 publications

(38 citation statements)

References 34 publications

(45 reference statements)

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“…For V1016I, variation in allele frequency has been observed among the populations analyzed, but other studies in the country have reported lower frequencies in the populations of Armenia, Montenegro and Calarca in the state of Quindio and Bello in the state of Antioquia and higher frequencies in the municipality of Villavicencio in the state of Meta . This last study also included the population of the municipality of Riohacha in the state of La Guajira with a frequency of 0.25, which is similar to that reported for the municipality of San Juan in the same state in the present study.…”

Section: Discussionsupporting

confidence: 78%

“…This indicates that the higher the frequency of these mutations, the higher the level of resistance to permethrin, λ‐cyhalothrin and cyfluthrin. The V1016I was the first kdr mutation reported for the species A. aegypti in Colombia; however, further studies have identified, in addition to this mutation, F1534C and V419L in the voltage‐dependent sodium channel in populations of this species in this country …”

Section: Discussionmentioning

confidence: 95%

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Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Maestre‐Serrano

Pareja-Loaiza

Camargo

et al. 2019

Pest Management Science

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BACKGROUND Knockdown resistance is conferred primarily by non‐synonymous mutations that reduce pyrethroids binding to voltage‐gated sodium channels. In 2014, kdr mutation V1016I in Aedes aegypti populations resistant to pyrethroids was reported for the first time in Colombiα, in 2016 another kdr mutation, F1534C, and in 2018 the mutation V419L. Nine populations of A. aegypti, previously characterized as being resistant to λ‐cyhalothrin, deltamethrin, cyfluthrin and permethrin, were used for this study. Genomic DNA was used to determine genotypes by allele‐specific PCR for mutations V1016I and F1534C, and to determine their association with pyrethroid resistance. RESULTS All the populations analyzed showed both mutations, with allelic frequencies of 0.07–0.35 for I1016 and 0.47–0.88 for C1534. A percentage of co‐occurrence of mutant homozygotes I1016/C1534 of 5.3% was detected. A significant positive correlation was found between the frequency of the genotype I1016 and the resistance to permethrin, λ‐cyhalothrin and cyfluthrin, but not to deltamethrin; on the other hand, the correlation was not significant for the C1534 genotype and the four pyrethroids evaluated. No significant correlation was found between the frequencies of the mutations V1016I and F1534C. CONCLUSIONS Both mutations V1016I and F1534C are present in A. aegypti populations of the Colombian Caribbean, and although the frequency of F1534C exceeds V1016I, the latter was correlated to resistance to pyrethroid insecticides. © 2018 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 95%

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Maestre‐Serrano

Pareja-Loaiza

Camargo

et al. 2019

Pest Management Science

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“…Similar consequences to the PY resistance together with an increase and spread of kdr alleles throughout North and South American Ae. aegypti populations [37, 47, 66, 67], may take place with Ae. albopictus as well.…”

Section: Discussionmentioning

confidence: 99%

“…DNA extraction followed Aguirre-Obando et al [47] guidelines. All the samples from each locality were individually extracted.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: First report of the Phe1534Cyskdr mutation in natural populations of Aedes albopictusfrom Brazil

2017

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BackgroundKnockdown resistance (kdr), caused by alterations in the voltage-gated sodium channel (NaV), is one of the mechanisms responsible for pyrethroid (PY) resistance. In the Asian tiger mosquito, Aedes albopictus, at least four different mutations were described in the IIIS6 NaV segment in populations from Asia, North America and Europe. In contrast, in Aedes aegypti at least 12 non-synonymous mutations have been reported at nine different codons, mostly in the IIS6 and IIIS6 NaV segments. The Phe1534Cys kdr mutation in the IIIS6 NaV segment is the most prevalent in populations of Ae. aegypti worldwide, also found in Ae. albopictus from Singapore. Herein, we investigated the DNA diversity corresponding to the IIS6 and IIIS6 NaV segments in natural populations of Ae. albopictus from Brazil.MethodsDNA from eight Brazilian Ae. albopictus natural populations were individually extracted and pooled by states of origin, amplified, cloned and sequenced for the corresponding IIS6 and IIIS6 NaV segments. Additionally, samples from each location were individually genotyped by an allelic specific PCR (AS-PCR) approach to obtain the genotypic and allelic frequencies for the 1534 NaV site.ResultsNo non-synonymous substitutions were observed in the IIS6 sequences. However, the Phe1534Cys kdr mutation was evidenced in the Ae. albopictus NaV IIIS6 segment sequences from Paraná (PR) and Rondônia (RO) states, but not from Mato Grosso (MT) state. The 1534Cyskdr allele varied from 3% (Marilena/PR and Porto Velho/RO) to 10% (Foz do Iguaçu/PR). To our knowledge, this paper reports the first occurrence and provides distribution data of a possible kdr mutation in Ae. albopictus in South America.ConclusionThe emergence of a likely kdr mutation in Ae. albopitus natural populations is a signal of alert for vector control measures since PY are the most popular insecticides adopted by residents. Additionally, once the kdr allele is present, its frequency tends to increase faster under exposition to those compounds. Although the Asian tiger mosquito is not incriminated as an important vector of dengue, chikungunya and Zika viruses in South America, its importance in this regard has been extensively discussed since Ae. albopictus is rapidly spreading and can also migrate between sylvatic and urban environments. Therefore, insecticide resistance monitoring initiatives should also be extended to Ae. albopictus in Brazil in order to maintain chemical compounds as an efficient vector control tool when needed.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2089-5) contains supplementary material, which is available to authorized users.

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“…Low genetic diversity is most likely a result of a decline in population size caused by insecticide use, as it was observed in American A. aegypti populations [72][73][74] . However, some studies have revealed the presence of greater genetic diversity in areas that are frequently treated with insecticides, as shown in A. aegypti populations from French Polynesia and Brazil [75][76] .…”

Section: Discussionmentioning

confidence: 88%

How much is known about the genetic diversity of the Asian tiger mosquito? A systematic review

Aguirre-Obando

Navarro-Silva

2017

revsal

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Introduction: Aedes (Stegomyia) albopictus (Skuse, 1894) is a vector for dengue and chikungunya viruses in the field, along with around 24 additional arboviruses under laboratory conditions. Knowledge of the genetic diversity of insect vectors is critical for the effective control and elimination of vector-borne diseases. Objective: We determined the current scenario of the genetic diversity in natural populations of A. albopictus through a systematic review. Methodology: It was possible to establish the first reports and distribution of A. albopictus populations in the world, as well as its genetic diversity, population genetic structure and molecular markers used to determine its genetic diversity. Results: A. albopictus is distributed worldwide with genetically structured populations and low diversity; however, 89.5% of the genetic diversity known is based on the use of RFLP, allozymes, isozymes, and mtDNA molecular markers that exhibit significant problems according to the literature. After the results were obtained, a critical analysis was carried out and existing shortcomings were detected. Conclusion: The current knowledge of genetic diversity of A. albopictus is based on genetic markers that exhibit significant problems reported in the literature; therefore, vector control programs targeting A. albopictus populations, may be compromised.

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Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Cited by 42 publications

References 34 publications

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

RETRACTED ARTICLE: First report of the Phe1534Cyskdr mutation in natural populations of Aedes albopictusfrom Brazil

How much is known about the genetic diversity of the Asian tiger mosquito? A systematic review

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