The diversity of Eimeria spp. in cattle in the Brazilian Semiarid region

Melo, Lídio Ricardo Bezerra de; Sousa, Luana Carneiro; Lima, Brendo Andrade; Silva, Ana Luzia Peixoto; Ferreira, Larissa Claudino; Feitosa, Thaís Ferreira; Vilela, Vinícius Longo Ribeiro

doi:10.1590/s1984-29612022037

Cited by 5 publications

(9 citation statements)

References 27 publications

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“…For the morphometric analysis, a LAB-DM300 digital microscope was used, coupled to a computer that was equipped with photomicrograph software suitable for the microscope used, which was capable of obtaining images with up to 3.2 million pixels. All photomicrographs were produced using 40 × and 100 × lenses (400 × and 1,000 × magnifications) and the measurements were made using the Mv Image ® software tools, similar to what was described by Araújo et al ( 18 ) and Melo et al ( 19 ).…”

Section: Methodsmentioning

confidence: 99%

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

et al. 2022

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In tropical regions, family farming is a form of production and work that is highly present in rural areas. Because the production system for free-range chickens has a low level of technification, it frequently presents massive infection by coccidia. The objective of this study was to determine the prevalence and diversity of Eimeria species in free-range chickens in northeastern Brazil. Fecal analyses were carried out using materials collected from 100 farms, belonging to 10 different municipalities. The sample from each farm was composed of five stool samples collected from different animals. Coproparasitological analyses were performed and, in each positive sample, photomicrographs of 20 oocysts were used for morphological identification of coccidia. The presence of Eimeria spp. was detected in 59% (59/100) of the farms analyzed. Species identification was performed through morphometric analysis of 1,180 sporulated oocysts. The following eight species of Eimeria spp. were found, in decreasing order of prevalence: Eimeria necatrix (25%), Eimeria mitis (18.3%), Eimeria mivati (17.3%), Eimeria tenella (12.4%), Eimeria brunetti (9.9%), Eimeria acervulina (9.1%), Eimeria praecox (4.8%) and Eimeria maxima (3.2%). The prevalence and diversity of Eimeria spp. on farms producing backyard chickens in the semiarid region of Brazil were high, especially the diversity of species. Changing the management, with the adoption of sanitary measures, may be effective in reducing the high prevalence of coccidia on the farms studied.

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Section: Methodsmentioning

confidence: 99%

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

et al. 2022

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“…This is related to the problem of wall resistance that has a double layer. The outer layer is thick and elastic and is composed of a chitin-like material, as well as tanned quinone proteins which hinder its disruption (Melo et al, 2022). Several techniques have been proposed for oocyst rupture, either mechanically or chemically, but a reproducible method for disrupting the oocyst wall and recovering high yields of DNA has not yet been developed (Oliveira et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In our study, 31/57 oocyst concentrations were negative in agarose gel. There are some factors, such as the quantity and quality of DNA, the presence of mixed Eimeria oocysts, contamination with PCR inhibitors, resistance of the oocyst wall to rupture or restricted access to template DNA, which can interfere with the molecular diagnosis of Eimeria in cattle (Oliveira et al, 2011;Lee et al, 2018;Ekawasti et al, 2022;Melo et al, 2022). Regarding the quantity and quality of the DNA, failures in the molecular identi cation of Eimeria spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Eimeria species in cattle from Veracruz, Mexico

Olivares

Alonso-Díaz

Romero-Salas

et al. 2023

Preprint

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The presence of Eimeria in livestock farms implies significant economic losses, compromising the welfare and animal health of affected bovines. The objectives of this study were to obtain the molecular identification of Eimeria species and to create the phylogeny for analysis. Fecal samples were collected from 930 animals distributed in 62 cattle farms located in the center of the state of Veracruz, Mexico. A total of 57 oocyst concentrations from cattle farms were used. In 57 of the 62 farms in this study, it was possible to carry out the concentration of oocysts. Extraction and PCR were created through the internal transcribed spacer region 1 (ITS-1) of ribosomal rRNA genes. Twenty-six of the 57 (45.6%) samples were amplified at 500 bp. The presence of pathogenic species such as Eimeria bovis, Eimeria zuernii, in addition to Eimeria ellipsoidalis, Eimeria cylindrica, Eimeria budkinonensis and Eimeria canadensis, infecting calves in the region was confirmed. The phylogenetic tree correctly differentiated the presence of seven Eimeria species, reporting incidentally the presence of paratenic hosts among production animals. It is concluded that these results can be useful to improve the management and productivity of cattle in the Mexican tropics.

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“…These helminths cause parasitic gastroenteritis in cattle (Micheloud et al, 2015;Henriques et al, 2021;Melo et al, 2021). Among enteric coccidia, Eimeria genera stands out because of its cosmopolitan distribution and parasitism in cattle worldwide, causing high levels of cases of coccidiosis, which can affect up to 100% of young cattle, especially during the first weeks of life (Faber et al, 2002;Gunathilaka et al, 2018;Hamid et al, 2019;Melo et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Resistant parasite populations hinder effective control in herds. Recently, anthelmintic multidrug resistance was described in cattle herds in the semi-arid region of northeastern Brazil (Melo et al, 2021) and several species of Eimeria parasitizing these animals were later identified (Melo et al, 2022). In such situations, the animals develop clinical symptoms of greater severity, and high mortality rates may occur, thus raising the economic losses (Almeida et al, 2020;Melo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological survey of gastrointestinal infections by gastrointestinal nematodes and coccidia in cattle in the semiarid region of Northeastern Brazil

et al. 2023

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The aim of the present study was to carry out an epidemiological survey on gastrointestinal infections by nematodes and coccidia in cattle herds in the semiarid region of Paraíba, northeastern Brazil. A total of 832 cattle were sampled, of both sexes and different ages, on 21 farms in 21 municipalities in the state of Paraíba, Brazil. Fecal samples were collected individually to perform eggs per gram (EPG) and oocysts per gram (OoPG) counts in feces, and to perform fecal cultures. To assess factors associated with nematode and coccidia infections, epidemiological survey forms were applied to the farmers. The total frequency of infections by nematodes was 59.6% (496/832) and it was 36.9% (307/832) for coccidia. The levels of infection by nematodes and coccidia were most frequently low (EPG < 300; OoPG < 1,000), in 72.4% (359/496) and 75.2% (231/307), respectively. The most frequent nematode genera was Haemonchus spp. (78.8%). The risk factors for nematode infections in cattle were beef production trait (odds ratio = 2.99) and extensive rearing system (odds ratio = 3.8); the factors for coccidia were age ≤ 12 months (odds ratio = 2.9) and body score between 1 and 2 (odds ratio = 5.2). These results prove that there are high frequencies of gastrointestinal infections by nematodes and coccidia in cattle herds in the semiarid region of Paraíba, with predominantly low levels of infection. However, given the importance of subclinical infections, adjustments to sanitary management measures can contribute to reduction of parasite indices and better performance among the animals, thus generating greater profitability for farmers.

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The diversity of Eimeria spp. in cattle in the Brazilian Semiarid region

Cited by 5 publications

References 27 publications

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

Molecular identification of Eimeria species in cattle from Veracruz, Mexico

Epidemiological survey of gastrointestinal infections by gastrointestinal nematodes and coccidia in cattle in the semiarid region of Northeastern Brazil

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