High genetic diversity of Anaplasma marginale infecting dairy cattle in northeastern Brazil

Pereira, José Gomes; Garcia, Amanda Barbosa; Gonçalves, Luiz Ricardo; Ramos, Inalda Angélica de Souza; Braga, Maria do Socorro Costa Oliveira; Santos, Larissa Sarmento dos; Melo, Ferdinan Almeida; Santos, Hamilton Pereira; Neta, Alcina Vieira de Carvalho; Rosário, Carla Janaina Rebouças Marques do; Lima, Cristian Alex Aquino; Carvalho, Robert Ferreira Barroso de; Silva, Lucas Diniz; Lima, Mauricio Sousa; André, Marcos Rogério; Machado, Rosangela Zacarias

doi:10.1590/s1984-29612021097

Cited by 2 publications

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References 37 publications

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“…These results are consistent with previous results for strains found mainly in Brazil ( 19 , 20 , 38 ), Argentina ( 18 ) and Colombia ( 39 ). Other genotypes frequently observed in our work were G and H. Studies carried out in Brazil (São Paulo and Maranhão) reported that genotype H was the most frequently found in beef and dairy cattle ( 21 , 22 ). However, the genetic diversity of circulating strains in the equatorial zone, comprising the countries of Ecuador, Mexico, Cuba, and subtropical countries such as South Africa and the USA, are different, with G and C being the main genotypes ( 15 , 27 , 40 ).…”

Section: Discussionsupporting

confidence: 60%

Characterization of strains of Anaplasma marginale from clinical cases in bovine using major surface protein 1a in Uruguay

et al. 2022

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The major surface protein 1a (MSP1a) gene has been used to characterize Anaplasma marginale genetic diversity. This pathogen causes significant productivity and economic losses to the cattle industry. The objective of the present study was to report the first characterization of A. marginale genetic diversity in Uruguay based on MSP1a genotypes and their putative relationship with Rhipicephalus microplus. This cross-sectional study was conducted between 2016 and 2020. The study included whole blood samples from clinical cases of bovine anaplasmosis obtained from 30 outbreaks located in six Uruguay territorial departments. Diagnosis was performed using Giemsa-stained smears and confirmed by nested Polymerase Chance Reaction (nPCR) targeting the A. marginale major surface protein 5 gene. The genetic diversity of A. marginale strains was characterized by analyzing the microsatellite and tandem repeats of MSP1a. Based on the microsatellite structure, four genotypes were identified. Genotype E was the most prevalent. Analysis of MSP1a tandem repeats showed 28 different strains from the combination of 31 repeats, with τ-10-15 and α-β-β-β-Γ being the most common. Repeats Γ, β, α, and γ were associated with the absence of R. microplus with statistical significance (p < 0.05). Molecular observations showed that 46.7% of the strains identified in our samples lacked the ability to bind to tick cells; therefore, they were probably transmitted by other vectors. Strain genetic diversity provides valuable information for understanding the epidemiological behavior of A. marginale and could contribute to the development of effective vaccines for the control of this disease.

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