Extract of Ascaris suum induces TGF-β and early production of IgG1 in experimental autoimmune hepatitis

Silva, Roeckson Carlos Peixoto; Silva, Raul Penaforte Correia da; Silva, Maria da Conceição; Nascimento, Wheverton Ricardo Correia do; Costa, Vlaudia Maria Assis; Albuquerque, Mônica Camelo Pessôa de Azevedo; Souza, Valdênia Maria Oliveira de

doi:10.1590/s1984-29612020031

Cited by 3 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, PAS‐1, a protein of A. suum , stimulated the production of IL‐10 and TGF‐β in murine model of asthma 27 . We observed that ASC induced IL‐4, IL‐10, IL‐13 and TGF‐β by spleen cells in EAH, besides IgG1 4,28 . This isotype of antibody can produce IL‐10 via IgG‐FcγR by macrophage 29,30 .…”

Section: Discussionmentioning

confidence: 65%

“…Given that activation of HSC and a proper balance between M1 and M2 macrophages are key events in the development of hepatic fibrosis, 14,15 it is possible that the effect of ASC towards suppressing M2 macrophages by impairing RNAm of Retnla / Fizz1 may not be superimposed on the activation potential of HSC. Moreover, ASC induced IL‐4, IL‐13 and TGF‐β, 4,28 which may favour activation of HSC and differentiation of M2 macrophages. This finding may explain the production of collagen that was observed in the EAH + ASC group.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Extract from Ascaris suum and N‐acetyl‐L‐cysteine induces an immunosuppressant effect in model of autoimmune hepatitis

Silva

Souto

et al. 2021

Parasite Immunology

Self Cite

View full text Add to dashboard Cite

Background Extract of adult Ascaris suum (ASC) worms attenuated the liver damage in experimental autoimmune hepatitis (EAH) with induction of Th2 immune response, but fibrosis occurred. N‐acetyl‐L‐cysteine (NAC) has protective effects against liver fibrosis. Objectives Evaluate the association ASC + NAC on the T‐ and B‐cell activation, inflammation and fibrogenic markers in the liver in EAH. Methods Experimental autoimmune hepatitis was induced intravenously with concanavalin A in BALB/c mice. EAH + ASC+NAC group received NAC and ASC; EAH + ASC group received ASC; EAH group received PBS. Doubly labelled CD4+ T (CD28, CTLA‐4, CD40L or IL‐10) and CD45R+ B lymphocytes (IL‐10) and CD4+CD25+FoxP3+ cells were evaluated, along with gene expression of Col1a1, α‐SMA, Fizz1, Arg1 and PPAR‐γ and histomorphometry. Results Experimental autoimmune hepatitis group showed high frequency of CD28+ and CD40L+ T lymphocytes, but not the EAH + ASC group. In relation to EAH group, the Fizz1 expression was lower in both groups treated, but Arg1 expression was lower in only EAH + ASC+NAC group. In the EAH + ASC+NAC group, there were higher frequencies of CD4+IL‐10+ and CD4+CD25+FoxP3+ cells, but not CD45R+IL‐10+, along with mitigated inflammation and collagen production. Conclusions Ascaris suum favoured immunosuppression in EAH limiting the T cells activation. However, association ASC and NAC was necessary for attenuating the inflammatory process and collagen production.

show abstract

Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 98%

Extract from Ascaris suum and N‐acetyl‐L‐cysteine induces an immunosuppressant effect in model of autoimmune hepatitis

Silva

Souto

et al. 2021

Parasite Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

The identification of signature genes and their relationship with immune cell infiltration in age-related macular degeneration

Chen,

Zhao,

Zhang

et al. 2024

Mol Biol Rep

View full text Add to dashboard Cite

The identification of signature genes and their relationship to immune cell infiltration in age-related macular degeneration

Chen¹,

Zhang²,

Luo³

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Age-related macular degeneration (AMD) is a prevalent source of visual impairment among the elderly population, and its incidence has risen in tandem with the increasing longevity of humans. Despite the progress made in anti-VEGF therapy, the clinical outcomes have proven to be unsatisfactory. Method We obtained differentially expressed genes (DEGs) of AMD patients and healthy controls from GEO database. GO analysis and KEGG analysis were used to enrich the differential genes. Weighted gene co-expression network analysis (WCGNA) is used to screen modules related to AMD expression. SVM, random forest, and least absolute shrinkage and selection operator (LASSO) were used to screen hub gene. Gene set enrichment analysis (GSEA) is used to explore the pathway through which these hub genes are enriched. CIBERSORT was used to analyze the relationship between hub gene and immune cell infiltration. Finally, West blotting and RT-PCR were used to explore the expression of Hub gene in AMD mice. Results We screened 1084 differential genes in GSE29801, of which 496 genes were up-regulated. 1084 differential genes were introduced into WCGNA analysis, and 94 genes related to AMD were obtained. 79 overlapping genes were obtained by VEEN plot. The 79 genes were introduced into three machine learning methods to screen the Hub gene, and the gene screened by the three methods was TNC,FAP,SREBF1,and TGF-β2. We verified their diagnostic function in GSE29801 and GSE103060 gene sets respectively. Then the pathway of hub gene co-enrichment was obtained by GO analysis and KEGG analysis. CIBERSORT analysis showed that these hub genes were associated with immune cell infiltration. Finally, we found increased expression of TNC, FAP, SREBF1, and TGF-β2 mRNA and protein in the retina of AMD mice. Conclusion We found that four hub genes, FAP, TGF-β2 and SREBF1,and TNC, have diagnostic significance in patients with AMD and are related to immune cell infiltration. Finally, we found up-regulated of these hub genes mRNA and protein in the retina of AMD mice.

show abstract

Extract of Ascaris suum induces TGF-β and early production of IgG1 in experimental autoimmune hepatitis

Cited by 3 publications

References 13 publications

Extract from Ascaris suum and N‐acetyl‐L‐cysteine induces an immunosuppressant effect in model of autoimmune hepatitis

Extract from Ascaris suum and N‐acetyl‐L‐cysteine induces an immunosuppressant effect in model of autoimmune hepatitis

The identification of signature genes and their relationship with immune cell infiltration in age-related macular degeneration

The identification of signature genes and their relationship to immune cell infiltration in age-related macular degeneration

Contact Info

Product

Resources

About