Molecular identification and histological aspects of Renicola sloanei (Digenea: Renicolidae) in Puffinus puffinus (Procellariiformes): a first record

Abstract

Renicolids are parasites that inhabit the renal tubules and ureters of molluscivorous and piscivorous birds. Puffinus puffinus is a migratory seabird that was identified as the definitive host of Renicola spp. Studies focusing on the renicolid species and the resulting renal lesions are valuable for their association with causes of stranding in seabirds. The aim of this study was to identify the renicolid trematodes and evaluate the histological findings in two P. puffinus stranded on the coast of Paraná state, Brazil. The parasites were evaluated by histologic, ultrastructural and molecular assays, while tissue changes were analyzed by histologic methods. The morphological and morphometrical characteristics of the parasites, along with polymerase chain reaction and sequencing assays (ribosomal and mitochondrial regions), identified the species as Renicola sloanei. The results also suggest that this helminth can be the adult form of Cercaria pythionike. The dilation of collecting ducts was the main histological finding in the kidneys. In conclusion, R. sloanei was identified, and for the first time, P. puffinus was described as a host of this digenean inducing mild renal changes.

Overview of renicolid digeneans (Digenea, Renicolidae) from marine gulls of northern Holarctic with remarks on their species statuses, phylogeny and phylogeography

“…At the same time, in all adults from cluster II described so far vitellaria are located lateral to the caeca in the middle third of the body, which is a characteristic feature of species from the pinguis -group in accordance with Wright (1957) and Odening (1962). Testes are contiguous [ R. thapari , R. sloanei Wright, 1954, Renicola pinguis (Mehlis in Creplin, 1846) Cohn, 1904, R. lari and R. keimahuri (Yamaguti, 1939; Caballero, 1953; Wright, 1954; Prevot and Bartoli, 1978; Rubio-Godoy et al ., 2011; Matos et al ., 2019; this study)] or fused to form a single mass, which is a diagnostic character of Nephromonorcha (Gibson, 2008). Renicola sternae is an exception: Fig.…”

“…Such a high diversity of cercarial morphotypes within a small family is unusual for trematodes (Galaktionov and Dobrovolskij, 2003). This matter apparently requires clarification, all the more so, as the results of molecular studies are ambiguous: some molecular data confirm that the larvae of the Rhodometopa group belong to renicolids (Matos et al, 2019), while other data indicate the opposite (Heneberg et al, 2016).…”

Seabirds: studies with parasitofauna and potential indicator for environmental anthropogenic impacts

“…The species Renicola spp., a digenetic trematode often observed in seabird kidneys and ureters (Wright, 1954a) (Figure 2), has been reported in studies with Brazilian birds, such as in Charadriiformes (Larus spp., Sterna spp., and Thalasseus maximus) (Wright, 1954b;Freitas, 1955;Thatcher, 1993), Suliformes (Sula spp.) (Freitas, 1955;Thatcher, 1993), Magellanic penguins (S. magellanicus) (Jerdy et al, 2016), and Manx shearwater (P. puffinus) (Mariani, 2016;Matos et al, 2019). In some species, the parasitic disease by renicolids may obstruct the tubular lumen or lead to more severe lesions such as nephritis, tubular hyperplasia, and fibrosis (Wright, 1954a;Munyer & Holloway, 1990;Jerdy et al, 2016).…”

Dinuclear lanthanide complexes supported by a hybrid salicylaldiminato/calix[4]arene-ligand: synthesis, structure, and magnetic and luminescence properties of (HNEt3)[Ln2(HL)(L)] (Ln = SmIII, EuIII, GdIII, TbIII)

“…There are only very few studies reporting thermodynamic data for f-element calixarene complexes in non-aqueous solvents. [93][94][95][96] Danil de Namor and Jafour have studied the complexation of trivalent cations by p-tert-butylcalix [4]arene tetraethanoate, p-tert-butylcalix [4]arene tetramethyl ketone, and p-tert-butylcalix [4]arene tetraacetamide in acetonitrile. 97 Borisova and co-workers have determined stability constants for lanthanide complexes supported by 2,2′-bipyridyl-6,6′-dicarboxylic acid diamide and 2,6-pyridinedicarboxylic acid diamide ligands in the same solvent.…”