2018
DOI: 10.1590/s1984-296120180079
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Abstract: Infection by the acantocephalan Neoechinorhynchus buttnerae is considered one of most important concerns for tambaqui fish (Colossoma macropomum ) production. Treatment strategies have been the focus of several in vivo studies; however, few studies have been undertaken on in vitro protocols for parasite maintenance. The aim of the present study was to develop the best in vitro culture condition for N. buttnerae to ensure its survival and adaptation out of the host to allow for the testing of substances to be u… Show more

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Cited by 12 publications
(7 citation statements)
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“…Parasites were then collected and placed in universal collectors containing RPMI 1640 medium (Gibco Laboratories, Grand Island, NY, United States of America ® ) for in vitro anthelmintic tests, as recommended by Costa et al . (2018).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Parasites were then collected and placed in universal collectors containing RPMI 1640 medium (Gibco Laboratories, Grand Island, NY, United States of America ® ) for in vitro anthelmintic tests, as recommended by Costa et al . (2018).…”
Section: Methodsmentioning
confidence: 99%
“…Collectors were incubated at 26°C in a biological oxygen demand incubator, in the temperature range (24–32°C) tested by Costa et al . (2018). In the optimum temperature range, there were no deaths of parasites in the RPMI medium.…”
Section: Methodsmentioning
confidence: 99%
“…After egg addition in the 12‐well plates (total volume of 1 ml), they were kept in a greenhouse at 28°C, as per the recommendation of Costa et al. (2018), and evaluated after 24 h.…”
Section: Methodsmentioning
confidence: 99%
“…The procedure for in vitro tests to evaluate anthelmintic activity of the ORs followed recommendations in Costa et al. (2018). First, naturally parasitized fish were euthanized with benzocaine (0.1 g/L) followed by medullary section.…”
Section: Methodsmentioning
confidence: 99%