Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Abstract

The aim of this study was to investigate the occurrence of anti-Leishmania spp. antibodies in dogs from localities in the city of Foz do Iguaçu, Paraná state, Brazil, on the border with Argentina and Paraguay. Blood samples dogs were collected to perform the following serologic tests: immunochromatographic DPP® rapid test, indirect immunoenzymatic assay (ELISA) and indirect immunofluorescence assay (IFA). In 2012, 285 dogs were analyzed on Argentina border, and in 2013, serum samples from 396 dogs on the border of Paraguay were collected. Using ELISA for screening and IFA for the confirmatory test, the results showed that the antibody prevalence was 1.8% (5/285) on the border of Argentina and 3.0% (12/396) on Paraguay border. When using the DPP® for screening and ELISA as a confirmatory analysis, we observed a seroreagent prevalence in dogs of 2.5% (7/285) on Argentina border and 5.1% (20/396) on Paraguay border. The non-public collection of domestic waste (p= 0.0004) was shown to be associated with leishmaniasis. This study shows the presence of leishmaniasis and suggest the emergence of canine visceral leishmaniasis in state of Paraná due to the confirmed occurrence of seroreactive dogs on Argentina and Paraguay border, which has environmental and geographical characteristics that favor the spread of the parasite.

Canine visceral leishmaniasis: perception, prevalence, and spatial distribution in municipality of Nossa Senhora do Livramento, Mato Grosso, Brazil

“…Continuing with environmental variables, the univariate analysis revealed that the lack of public garbage collection was a risk factor for CVL. Such a result was also reported to contribute to a higher risk for canine and human disease in a previous study (Dias et al, 2018;Lima et al, 2018). According to Oliveira & Araújo (2003), residues in the peridomicile can serve as a shelter and breeding ground for vectors, which may explain why previous occurrence of CVL in the neighborhood in our study was a risk factor for infection.…”

First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil

“…Also, biological characterization of parasite isolates and continuous vector surveillance is thus necessary towards decision-making for appropriate actions for controlling this disease transmission among dogs, thereby avoiding the transmission of VL to humans. (Dias et al, 2018).…”

The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs

“…Leishmania braziliensis is endemic in the study area, and L. infantum has been recognized since 2012 (Dias et al, 2013;Thomaz Soccol et al, 2017); therefore, to work with specific species of CVL, dogs were subjected to clinical evaluation and identification of the parasite present in the popliteal ganglion. In endemic areas of L. braziliensis, diseased dogs generally have typical well-limited ulcerated lesions with raised edges.…”