Immunohaematological and rheological parameters in canine visceral leishmaniasis

Silva, José Nivaldo da; Cotrim, Aron Carlos de Melo; Conceição, Luciana Auxiliadora Viebrantz da; Marins, Camila Moreira Ferreira; Marchi, Patrícia Gelli Feres de; Honório-França, Adenilda Cristina; Almeida, Arleana do Bom Parto Ferreira de; França, Eduardo Luzía; Sousa, Valéria Régia Franco

doi:10.1590/s1984-296120180021

Cited by 2 publications

(12 citation statements)

References 31 publications

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“…[7], and another that demonstrated changes in blood viscosity of dogs infected with Leishmania ssp. [9].…”

Section: Hemorheological Diagnosismentioning

confidence: 99%

“…Rheometric blood analysis or hemoremometry is a technique for measuring blood viscosity that helps in understanding the pathogenesis of diseases affecting the blood [9]. Blood functions as a viscous fluid, with different viscosities depending on the amount of cells, platelets, and other blood solutes [114,115], so if a disease alters the amount of cells, the deformability erythrocyte or serum components, the viscosity also changes.…”

Section: Rheologymentioning

confidence: 99%

“…Metabolic diseases such as diabetes mellitus lead to erythrocyte changes [118], in addition to other factors such as increased serum osmolarity [119] and endothelial lesions lead to blood hyperviscosity syndrome [120]. In infectious diseases, such as those caused by obligate intracellular parasites, increased blood viscosity occurs, as observed in dogs with Canine Visceral Leishmaniasis [9] and in humans with malaria [7].…”

Section: Rheologymentioning

confidence: 99%

“…Infectious diseases may alter the hematological parameters of the affected individuals and, consequently, there is alteration of hemorheological behavior [7][8][9]. In addition, immunological factors are also responsible for the change in blood viscosity.…”

Section: Introductionmentioning

confidence: 99%

“…Studies on the hemorheological behavior of dogs with infectious diseases, as well as the immunomodulation of this process help to understand the immunophysiopathological mechanisms [7,9].…”

Section: Introductionmentioning

confidence: 99%

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Hemorheological Evaluation and Cytokine Production in Dogs Naturally Infected with Anaplasmataceae

Cardoso¹,

Paludo²,

Silva³

et al. 2020

Parasitology and Microbiology Research

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In this chapter, we describe that naturally infected dogs with Anaplasmataceae show altered rhreological parameters. Also, we have showed that lower viscosity correlated with the lower erythrocyte number and release of IFN-γ. The rheometry of the fresh blood samples was measured by using the Modular Compact Rheometer-MCR 102 (Anton Paar® GmbH, Ostfildern, Germany), and the graphs were obtained using Rheoplus software. Blood count data were obtained by analysis in a private laboratory. Diagnostic confirmation was obtained by molecular PCR technique that was used to determine the groups of not infected and infected by Anaplasmataceae. Serum cytokines were dosed by flow cytometry (FACScalibur BD®) using BD® Biosciences Cytometric Bead Array (CBA) Human Th1/Th2/Th17 Cytokine kits. The results showed a correlation between blood viscosity (p < 0.05, r = 0.73) and shear rate (p < 0.05; r = −0.676) with IFN-γ in the group of infected dogs that presented anemia, as well as correlations of shear rate with erythrocytes (p < 0.05; r = −0.88). Thus, IFN-γ appears to play an important role in the immunomodulation of the rheological behavior of naturally infected dogs to Anaplasmataceae. The alterations in cytokines profile and their relationship with blood viscosity and hematological parameters was related in this study the first time of dogs naturally infected with Anaplasmataceae.

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“…[7], and another that demonstrated changes in blood viscosity of dogs infected with Leishmania ssp. [9].…”

Section: Hemorheological Diagnosismentioning

confidence: 99%

Section: Rheologymentioning

confidence: 99%

Section: Rheologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Studies on the hemorheological behavior of dogs with infectious diseases, as well as the immunomodulation of this process help to understand the immunophysiopathological mechanisms [7,9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hemorheological Evaluation and Cytokine Production in Dogs Naturally Infected with Anaplasmataceae

Cardoso¹,

Paludo²,

Silva³

et al. 2020

Parasitology and Microbiology Research

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Effects of Doxycycline Treatment on Hematological Parameters, Viscosity, and Cytokines in Canine Monocytic Ehrlichiosis

Cardoso

Honório-França

França

et al. 2023

Biology

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This study aimed to analyze the hematological parameters, blood viscosity, and cytokines of dogs infected by Ehrlichia canis untreated and treated with doxycycline. Initially, 47 dogs were examined, and 36 were suspected to have canine monocytic ehrlichiosis, which was confirmed through molecular polymerase chain reaction tests. This study consisted of 25 dogs, with 11 being healthy and 14 testing positive for E. canis. The dogs were divided into experimental groups based on their test results, including a control group of healthy dogs (N = 11), a group of infected dogs without treatment (N = 7), and a group of infected dogs treated with doxycycline (N = 7) at a 10 mg/kg dose every 12 h for 28 days. Blood samples were taken to determine hematological parameters, viscosity, and cytokine levels. It was observed that, regardless of doxycycline treatment, there was a reduction in total leukocytes and lymphocytes in infected dogs with Ehrlichia canis. The eosinophils and platelets decreased in dogs with Ehrlichia canis infections without treatment. Monocytes, eosinophils, and platelets increased when the dogs were treated with doxycycline. Regardless of treatment, infected dogs’ blood viscosity was lower than uninfected dogs. Infected dogs showed lower TNF-α and increased IL-1β. There was a correlation between the blood viscosity with the cytokines IL-10 and IL-12 in the infected dogs. The eosinophil count correlated with TNF-α in the group of infected and untreated dogs. In conclusion, treating dogs with monocytic ehrlichiosis using doxycycline can increase platelet and eosinophil levels but may also increase IL-1β and monocyte levels, exacerbating inflammation. Therefore, evaluating viscosity and cytokine levels is important when treating dogs with this condition.

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Immunohaematological and rheological parameters in canine visceral leishmaniasis

Cited by 2 publications

References 31 publications

Hemorheological Evaluation and Cytokine Production in Dogs Naturally Infected with Anaplasmataceae

Hemorheological Evaluation and Cytokine Production in Dogs Naturally Infected with Anaplasmataceae

Effects of Doxycycline Treatment on Hematological Parameters, Viscosity, and Cytokines in Canine Monocytic Ehrlichiosis

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