Molecular detection of Anaplasmataceae agents in Dasyprocta azarae in northeastern Brazil

Braga, Maria do Socorro Costa de Oliveira; Pereira, José Gomes; Fernandes, Sérgio Rodrigo; Marques, Ingrid Carolinne Lopes; Jesus, Renata Passos de; Ferreira, Gleycianny Santos; Xavier, Daniele Rosa; Benevenute, Jyan Lucas; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.1590/s1984-29612017071

Cited by 5 publications

(7 citation statements)

References 47 publications

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“…The rrs genotypes phylogenetically related to E. canis have been increasingly described in wild animals from Brazil, and have already been detected in wild canids 12 , 14 , wild felids 15 , rodents 14 , 18 , 19 , coatis 14 , and geese ( Neochen jubata ) 24 . E. minasensis , a recently described species that is genetically similar to E. canis 62 , 63 , can infect and cause clinical signs in cattle in central-western Brazil 64 .…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, several genotypes of A. phagocytophilum , A. marginale , A. bovis , A. platys , E. canis , E. chafeensis , and potential new species, that are not yet named, have been detected in different species of wild animals, including deer 5 – 11 , wild canids 12 – 14 , wild felids 12 , 15 , 16 , coatis 14 , rodents 14 , 17 – 19 , caititus and peccaries 20 , opossums 21 , 22 , and birds 23 , 24 .…”

Section: Introductionmentioning

confidence: 99%

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Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’

Calchi

Vultão

Alves

et al. 2020

Sci Rep

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Anaplasmataceae agents are obligatory intracellular Gram-negative α-proteobacteria that are transmitted mostly by arthropod vectors. Although mammals of the Superorder Xenarthra (sloths, anteaters, and armadillos) have been implicated as reservoirs for several zoonotic agents, only few studies have sought to detect Anaplasmataceae agents in this group of mammals. This study aimed to investigate the occurrence and genetic diversity of Anaplasma spp. and Ehrlichia spp. in blood and spleen samples of free-living Xenarthra from four different states in Brazil (São Paulo, Mato Grosso do Sul, Rondônia, and Pará). Nested and conventional PCR screening assays were performed to detect the rrs and dsb genes of Anaplasma spp. and Ehrlichia spp., respectively. The assays were positive in 27.57% (91/330) of the Anaplasma spp. and 24.54% (81/330) of the Ehrlichia spp. Of the 91 positive Anaplasma spp. samples, 56.04% were positive in a conventional PCR assay targeting the 23S–5S intergenic region. Phylogenetic and distance analyses based on the rrs gene allocated Anaplasma sequences from sloths captured in Rondônia and Pará states in a single clade, which was closely related to the A. marginale , A. ovis, and A. capra clades. The sequences detected in southern anteaters from São Paulo were allocated in a clade closely related to sequences of Anaplasma spp. detected in Nasua nasua , Leopardus pardalis , and Cerdocyon thous in Brazil. These sequences were positioned close to A. odocoilei sequences. Genotype analysis corroborated previous findings and demonstrated the circulation of two distinct Anaplasma genotypes in animals from north and southeast Brazil. The first genotype was new. The second was previously detected in N. nasua in Mato Grosso do Sul state. The intergenic region analyses also demonstrated two distinct genotypes of Anaplasma . The sequences detected in Xenarthra from Pará and Rondônia states were closely related to those in A. marginale , A. ovis, and A. capra . Anaplasma spp. sequences detected in Xenarthra from São Paulo and were allocated close to those in A. phagocytophilum . The analyses based on the dsb gene grouped the Ehrlichia spp. sequences with sequences of E. canis (São Paulo, Mato Grosso do Sul, and Pará) and E. minasensis (Rondônia and Pará). The data indicate the occurrence of E....

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’

Calchi

Vultão

Alves

et al. 2020

Sci Rep

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“…Cities and suburbs are home to a large number of pigeons of various breeds, including wild pigeons, which are permanent residents of parks, playgrounds, markets and other facilities and can serve as distributors of the most common intestinal nematodosis (Vaz et al, 2017;El-Dakhly et al, 2019). The presence of nematodes in some organs (liver, trachea and lungs) of infected pigeons can have serious consequences for human health, especially when eating pigeons.…”

Section: Introductionmentioning

confidence: 99%

Parasites of domestic and wild pigeons in the south of Ukraine

et al. 2021

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Pigeons are closely related to human life and are both a source of food and object for hobbies and sports. Parasitic diseases of birds are the main reason for their growth retardation, reduced productivity and viability. The article presents the results of studying the prevalence of trichomonosis, cestodes and nematodes among the population of wild and domestic pigeons in the south of Ukraine. According to the results of the conducted researches it is established that in the south of Ukraine endoparasitoses of domestic and wild pigeons are quite widespread, and all species of this bird are carriers of Trichomonas gallinae. At the same time, the highest rate of Trichomonas infection was recorded in Columba palambus and C. livia domestica, and the overall infestation of males is higher by 4.4% compared to females. It was determined that domestic pigeons C. livia domestica were infested with trichomonosis (27.5%) and nematodes (Ascaridia columbae, 57.1%), C. palambus – trichomonosis (32.1%) and cestodes (Raillietina spp., 82.4%), Streptopelia turtur – cestodes (Raillietina spp., 12.5%) and S. decaocto – cestodes (Raillietina spp., 71.4%) and nematodes (Heterakis gallinarum, A. columbae – 33.3% and 44.4% respectively). The overall rate of infestation of pigeons with cestodes was 28.4% and the incidence in males was higher by 3.9% compared to females. It was found that the most common species among pigeon cestodes is Raillietina spp. In addition, 22.9% of pigeons are carriers of nematodes (H. gallinarum, A. columbae and Capillaria spp.). Continuous monitoring of pigeon parasites is necessary because they, in most cases, come into contact with other species of poultry and are a source of general invasion. In terms of further research, it would be promising to study the prevalence of helminthic infestation among wild migratory birds.

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“…Palavras-chave: Gambá, Didelphis aurita, Ehrlichia, erliquiose, caracterização molecular, Brasil. WERTHER et al, 2017;BENEVENUTE et al, 2017;BRAGA et al, 2018;SOUSA et al, 2017). However, to date there are few published studies on the occurrence of Anaplasmataceae in opossums.…”

Section: Introductionmentioning

confidence: 99%

“…Among wild animals, genotypes closely related to E. canis have been reported in wild carnivores (ANDRÉ et al, 2010(ANDRÉ et al, , 2012, wild birds from Brazil (MACHADO et al, 2012), Orinoco geese (Neochen jubata) (WERTHER et al, 2017), rodents (BENEVENUTE et al, 2017BRAGA et al, 2018), and wild mammals (SOUSA et al, 2017) from Brazil.…”

Section: Introductionmentioning

confidence: 99%

Detection of a putative novel genotype of Ehrlichia sp. from opossums (Didelphis aurita) from Brazil

Guimarães

Raimundo

Silva

et al. 2019

Rev. Bras. Parasitol. Vet.

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Erlichiosis affects humans and animals worldwide. Its distribution and prevalence depends on the presence of tick vectors and hosts in one geographic area. The aim of the present study was to investigate the occurrence of Ehrlichia spp. and Anaplasma spp. in opossums (Didelphis sp.) from the State of Rio de Janeiro, southeast Brazil. Blood samples from 37 animals were tested for these two pathogens using molecular methods. One animal (2.7%) was positive for Ehrlichia sp. by 16S rRNA-based nested PCR. In a phylogenetic analysis based on the 16S rRNA gene using the maximum likelihood method and the GTRGAMMA+I evolutionary model, we detected a novel Ehrlichia sp. genotype closely related to genotypes of E. canis previously reported in dogs from Brazil. To the authors’ knowledge, this is the first molecular detection of Ehrlichia sp. in opossums from this State in the southeastern region of the country.

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Molecular detection of Anaplasmataceae agents in Dasyprocta azarae in northeastern Brazil

Cited by 5 publications

References 47 publications

Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’

Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’

Parasites of domestic and wild pigeons in the south of Ukraine

Detection of a putative novel genotype of Ehrlichia sp. from opossums (Didelphis aurita) from Brazil

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