Hepatozoon caimani in Caiman crocodilus yacare (Crocodylia, Alligatoridae) from North Pantanal, Brazil

Bouer, A.; André, Marcos Rogério; Gonçalves, Luiz Ricardo; Luzzi, Mayara de Cássia; Oliveira, Juliana; Rodrigues, Ana Carolina; Varani, Alessandro de Melo; Miranda, Vitor F. O.; Perles, Lívia; Werther, Karin; Machado, Rosangela Zacarias

doi:10.1590/s1984-29612017041

Cited by 13 publications

(9 citation statements)

References 37 publications

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“…( B ) Haplotypic network of Hepatozoon sp. generated through mitochondrial gene 18S rRNA sequencing with TCS v.1.21 software 30 . Each line in the network represents a single mutational step; Small circles indicate hypothetical haplotypes that are necessary intermediates among the identified haplotypes, but which were not observed in the sampling.…”

Section: Resultsmentioning

confidence: 99%

“…In samples positive for both of the above PCR protocols, the two Hepatozoon 18S rRNA sequences obtained were concatenated to obtain a large 18S rRNA fragment to be used in phylogenetic analyses (approximately 1400 bp). Hepatozoon caimani DNA obtained from a naturally infected Caiman crocodilus yacare 30 was used as a positive control. Ultra-pure sterile water (Life Technologies ® , Carlsbad, CA, USA) was used as a negative control in all PCR assays.…”

Section: Methodsmentioning

confidence: 99%

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Genetic diversity of Hepatozoon spp. in rodents from Brazil

Perles

Roque

D’Andrea

et al. 2019

Sci Rep

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Hepatozoon spp. are Apicomplexan protozoa that parasitize a wide diversity of vertebrate hosts. In Brazil, few studies have reported the occurrence of Hepatozoon spp. in rodent species. Additionally, an evaluation of the population structure and distribution of Hepatozoon species over several Brazilian biomes has not yet been performed. The present work aimed to investigate the genetic diversity of Hepatozoon spp. in rodents from 31 genera sampled in five Brazilian biomes. Samples were submitted to PCR assays for Hepatozoon spp. targeting two regions of the 18S rRNA gene. Infection by Hepatozoon spp. was detected in 195 (42.2%) rodents comprising 24 genera. Phylogenetic analyses of 18S rRNA sequences grouped all sequences in the clade of Hepatozoon spp. previously detected in rodents and reptiles, apart from those detected in domestic/wild carnivores. These data raise two non-exclusive hypotheses: (i) rodents play an important role as intermediate or paratenic hosts for Hepatozoon infections in reptiles; and (ii) rodents do not seem to participate in the epidemiology of Hepatozoon infections of domestic/wild canids and felids in Brazil. TCS analyses performed with available 18S rRNA Hepatozoon sequences detected in rodents from Brazil showed the occurrence of six haplotypes, which were distributed in two large groups: one from rodents inhabiting the coastal region of Brazil and Mato Grosso state, and another from rodents from the central region of the country. A wide survey of the South American territory will help to elucidate the evolutionary history of Hepatozoon spp. parasitizing Rodentia in the American continent.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genetic diversity of Hepatozoon spp. in rodents from Brazil

Perles

Roque

D’Andrea

et al. 2019

Sci Rep

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“…The DNA positive controls for Leishmania infantum (JUSI et al, 2015), Bartonella henselae (André et al, 2015), Mycoplasma sp. (GONÇALVES et al, 2015), Hepatozoon caimani (BOUER et al, 2017), Babesia vogeli (Jaboticabal strain), Cytauxzoon sp. (ANDRÉ et al, 2009), Ehrlichia canis (Jaboticabal strain), and Anaplasma sp.…”

Section: Fvbp Molecular Screening and Characterizationmentioning

confidence: 99%

Molecular detection of vector-borne agents in cats in Southern Brazil

Pedrassani

Biolchi

Gonçalves

et al. 2019

Rev. Bras. Parasitol. Vet.

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This study used serological and molecular methods to investigate the occurrence of vector-borne pathogens (VBP) with zoonotic potential in cats neutered at the University Veterinary Hospital in Canoinhas, Santa Catarina. The combined PCR and serological results revealed that 17 (56.6%) cats were positive for one or more pathogens. The sampled cats had antibodies to Ehrlichia spp. (7/30), Anaplasma phagocytophilum (3/30) and Leishmania infantum (2/30). The PCR assay detected DNA closely related to Ehrlichia canis in 6/30 cats, Mycoplasma haemofelis in 2/30 cats, A. phagocytophilum and Cytauxzoon sp. in one cat each. While Bartonella clarridgeiae and B. henselae were detected in two cats each, and B. koehlerae was detected in one cat.

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“…In addition, blood smear analyses to detect hemoparasites revealed that 26% (6/23) of the animals from site 1 were infected with hemogregarine sensu lato, whereas no hemoparasites were detected in animals from site 2. Conversely, Bouer et al found a higher prevalence of Hepatozoon caimani in captive animals (88.8%; 8/9) than in free‐living animals (70.8%; 20/24) from the northern region of Pantanal (Brazil).…”

Section: Discussionmentioning

confidence: 96%

The effects of sex, body length, and different habitats on the serum protein electrophoresis of the broad‐snouted caiman (Caiman latirostris)

Fonseca

Dornelas

Orozco

et al. 2020

Veterinary Clinical Pathol

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Background Knowledge of mammalian inflammatory responses is vast; however, many aspects of the inflammatory response in non‐mammalian vertebrates, such as reptiles, remain unclear, including those regarding acute‐phase proteins (APPs). Recent studies have focused on the use of serum protein electrophoresis (SPE) to assess inflammatory responses in the broad‐snouted caiman (Caiman latirostris) and other reptiles. Objectives The purpose of this study was to examine the effects of sex, body length, and different habitats on SPE patterns in C latirostris using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Methods A total of 40 animals of both sexes and varying body lengths were collected; of these, 23 were free‐living in an industrial complex (site 1), and 17 were captive on a rural property (site 2). Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis was performed to fractionate different serum protein constituents. Results Sex affected protein levels, with females showing higher serum levels of total protein, as well as the 90‐, 66‐, and 58‐kDa proteins compared with males. Similarly, body length affected protein levels, with male adults >1.70 m in length showing the lowest serum levels of 152‐, 58‐, 54‐, and 27‐kDa proteins of all other animals. Finally, habitat affected protein levels, with animals at site 1 showing higher serum levels of 152‐ and 41‐kDa proteins compared with those at site 2. Conclusions This preliminary study was the first to evaluate the SPE of C latirostris using SDS‐PAGE. Further studies to identify the proteins in each band with more specific and sensitive techniques (eg, mass spectrometry) should be conducted to elucidate the standard of APPs in reptiles better.

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Hepatozoon caimani in Caiman crocodilus yacare (Crocodylia, Alligatoridae) from North Pantanal, Brazil

Cited by 13 publications

References 37 publications

Genetic diversity of Hepatozoon spp. in rodents from Brazil

Genetic diversity of Hepatozoon spp. in rodents from Brazil

Molecular detection of vector-borne agents in cats in Southern Brazil

The effects of sex, body length, and different habitats on the serum protein electrophoresis of the broad‐snouted caiman (Caiman latirostris)

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