New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil

Csordas, Bárbara Guimarães; Garcia, Marcos Valério; Cunha, Rodrigo Casquero; Giachetto, P. F.; Blecha, I. M. Z.; Andreotti, Renato

doi:10.1590/s1984-29612016053

Cited by 27 publications

(20 citation statements)

References 31 publications

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“…Cox1 is a well-known genetic barcode that could be utilized to molecularly identify tick species, and using this unique gene have improved epidemiological and evolutional studies that deal with ticks (9). Therefore; the Cox1-based recognition of ticks helps entomologists reaching the species level of ticks and even more to differentiate between closely related isolates (10). Moreover, knowing the species of ticks using this gene helps to recognize the breeds of animals that these ticks infest on, and if there is any alteration in the tick adaption to parasitize on different animal breeds (11).…”

Section: Introductionmentioning

confidence: 99%

Morphological and phylogenetic study of Hyalomma anatolicum in Al-Najaf, Iraq

Alfatlawi¹,

Ali²,

Albayati³

2019

IJVS

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Studies had been previously conducted to genetically identify species of ticks in Iraq. Therefore, the current investigational study was conducted to recognize the species of 50 ticks collected from infested skin of cattle. The current study defined the ticks to be from Hyalomma genus depending on their morphological features. Using mitochondrial cytochrome oxidase subunit  (Cox) gene, 16 ticks were further confirmed using polymerase chain reaction (PCR). Two PCR products were subjected to DNA sequencing to name the species of the ticks and compare them to some other known ticks in neighbor and world countries. The sequencing results identified the ticks to be Hyalomma anatolicum. One isolate is closely similar to Indian and Iranian isolates, and the other isolate is clustered alone by itself. The results indicated that H. anatolicum is one of the widespread ticks that affect cattle in Al Najaf province, Iraq.

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Section: Introductionmentioning

confidence: 99%

Morphological and phylogenetic study of Hyalomma anatolicum in Al-Najaf, Iraq

Alfatlawi¹,

Ali²,

Albayati³

2019

IJVS

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“…Additional polymorphisms may have occurred during the evolution of the R. microplus populations (SOSSAI et al, 2005). Population studies performed by Csordas et al (2016) with the cytochrome c oxidase subunit 1 (COI-1) gene indicated the presence of two haplotypes that differ from other Brazilian R. microplus populations. Thus, their antigenic epitopes may have become distinct from the epitopes of other populations.…”

Section: Discussionmentioning

confidence: 99%

“…The morpho taxonomy of Rhipicephalus microplus complex has been challenged in recent years, and until now, they are known as a complex composed of five taxa, namely R. australis, R. annulatus, R. microplus clade A sensu, R. microplus clade B sensu and R. microplus clade C sensu (BURGER et al, 2014;LOW et al, 2015;CSORDAS et al, 2016). This complex causes significant economic losses to livestock.…”

Section: Introductionmentioning

confidence: 99%

“…Vaccines, such as TickGARD PLUS (formerly, Intervet Australia), against the cattle tick in Australia, which was previously named R. microplus until it was morphologically and genetically distinguished from Rhipicephalus australis and now is part of the R. microplus complex (LABRUNA et al, 2009;ESTRADA-PEÑA, et al 2012;LOW et al, 2015;CSORDAS et al, 2016) are effective against susceptible strains of Australian origin. Numerous tests have been performed and might exhibit a different pattern of sensitivity and therefore induce poorer control when applied against R. microplus in America (SUTHERST & BOURNE, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Bm86 conserved epitopes: is a global vaccine against Cattle Tick Rhipicephalus microplus possible?

Blecha

Csordas

Aguirre

et al. 2018

Rev. Bras. Parasitol. Vet.

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The cattle tick Rhipicephalus microplus causes significant economic losses in agribusiness. Control of this tick is achieved mainly through the application of chemical acaricides, often resulting in contamination of animal food products and of the environment. Another major concern associated with acaricide use is the increasing reports of resistance of this tick vector against the active ingredients of many commercial products. An alternative control method is vaccination. However, the commercially available vaccine based on a protein homologous to Bm86 exhibits variations in efficacy relative to the different geographical locations. This study aimed to identify antigenic determinants of the sequences of proteins homologous to Bm86. Phylogenetic analyses were performed to determine the extent of divergence between different populations of R. microplus to identify the sequence that could be used as a universal vaccine against the multiple geographically distinct populations of R. microplus and related tick species. Considering the extensive sequence and functional polymorphism observed among strains of R. microplus from different geographical regions, we can conclude that it may be possible to achieve effective vaccination against these cattle ticks using a single universal Bm86-based antigen.

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“…The A. scalpturatum male was subjected to DNA extraction using the guanidine isothiocyanate and phenol/chloroform protocol, as described previously (SANGIONI et al, 2005adapted). Polymerase chain reaction (PCR) used primers ITS-2F and ITS-2R (CSORDAS et al, 2016) to target an rDNA fragment of 649 bp from the Internal Transcribed Spacer 2 (ITS2) gene for further sequencing.…”

Section: Molecular Analysis Of a Scalpturatummentioning

confidence: 99%

Amblyomma scalpturatum Neumann, 1906 (Acari: Ixodidae): confirmation in Acre State, Brazil, and description of parasitism in a human

Aguirre

Rodrigues

Costa

et al. 2019

Rev. Bras. Parasitol. Vet.

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Amblyomma scalpturatum is a tick species that is unique to South America. It is commonly associated with the Amazon biome and has been reported in some Brazilian states. This tick species exhibits host specificity: it parasitizes tapirs and suidae. Its role in transmitting pathogens to humans is still unknown. Amblyomma scalpturatum is known to be a human-biting tick; however, there is only one report showing that humans make suitable hosts for this species. The knowledge of tick fauna is lacking in the Acre State. This study collected free-living ticks with the aim of finding new records in Acre State. Collections were carried out in Amazon forest fragments in Rio Branco municipality. An A. scalpturatum specimen was identified and submitted sequencing of the ITS-2 gene. This study presents the first molecular confirmation of A. scalpturatum collected in situ in Acre State, North Region, Brazil. This study also presents the first record of a successfully completed feeding by an A. scalpturatum nymph on a human host in the North region of Brazil. Further investigation is needed to ascertain the complete life cycle of this tick species, its seasonality in the environment, and its relationship to pathogens and competence to transmit them. ResumoAmblyomma scalpturatum é uma espécie de carrapato que ocorre apenas na América do Sul. Está comumente associada ao bioma da Amazônia e tem sido relatada em alguns estados brasileiros. Esta espécie de carrapato apresenta especificidade a hospedeiros: parasita antas e suídeos. Sua competência em transmitir agentes patogênicos a seres humanos é ainda desconhecida. Amblyomma scalpturatum é também conhecido por picar seres humanos; entretanto, há apenas um relato que evidencie que seres humanos sejam capazes de serem hospedeiros adequados. Ainda existem lacunas no conhecimento sobre a ixodofauna no estado do Acre. Neste estudo foram coletados carrapatos de vida livre com o objetivo de encontrar novos registros para o estado do Acre. Coletas de carrapatos foram realizadas em fragmentos de floresta Amazônica no município de Rio Branco. Um exemplar de A. scalpturatum foi identificado e submetido a sequenciamento do gene ITS-2. Este estudo apresenta a primeira confirmação molecular de A. scalpturatum coletado in situ no estado do Acre, região Norte, Brasil. Este estudo também apresenta o primeiro relato de parasitismo completo de uma ninfa de

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New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil

Abstract: The Rhipicephalus (

Cited by 27 publications

References 31 publications

Morphological and phylogenetic study of Hyalomma anatolicum in Al-Najaf, Iraq

Morphological and phylogenetic study of Hyalomma anatolicum in Al-Najaf, Iraq

Analysis of Bm86 conserved epitopes: is a global vaccine against Cattle Tick Rhipicephalus microplus possible?

Amblyomma scalpturatum Neumann, 1906 (Acari: Ixodidae): confirmation in Acre State, Brazil, and description of parasitism in a human

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