Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites

Abstract

Differences in the efficacy of diagnostic techniques employed in the parasitological examination of feces are a limiting factor of this laboratory procedure in the field of Veterinary Parasitology. To verify advances in this type of examination in dogs, we conducted a study using a new technique (TFGII/Dog). Fifty naturally infected dogs were housed in individual stalls, and their feces were evaluated comparatively using this technique and four other conventional techniques. The TFGII/Dog showed high levels of sensitivity and efficiency, surpassing the diagnostic accuracy of the other techniques with a kappa concordance index of 0.739 (Substantial), as opposed to 0.546 (Moderate), 0.485 (Moderate), 0.467 (Moderate), and 0.325 (Fair) of the Spontaneous-Sedimentation, Centrifugal-Flotation in Saturated Zinc Sulfate Solution, Centrifugal-Flotation in Saturated Sugar Solution, and Spontaneous-Flotation in Saturated Sodium Chloride Solution techniques, respectively. The combination of positive results of all techniques comprises eight genera of parasites, with Ancylostoma spp. predominating among helminths, and Cystoisospora spp. among protozoa. The TFGII/Dog technique showed better diagnostic performance, and can therefore be considered an important tool for optimizing the results of laboratory routines and for the control of canine gastrointestinal parasites.

Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

“…1 and 2). The same could not be demonstrated in most slides processed by the CSN-Malachite technique, in accordance with literature reports (Coelho et al, 2015).…”

“…The precursor technique of this study, called TF-Test (Three Fecal Test), has been used with practicality for years for fecal parasitological diagnosis in humans, with excellent results (Gomes et al, 2004;Carvalho et al, 2012Carvalho et al, , 2016. More recently, this technique has been extended to research in animal area by studies involving sheep (Lumina et al, 2006) and canine (Coelho et al, 2013(Coelho et al, , 2015 species. In these works, new diagnostic procedures were validated with gastrointestinal helminths and protozoa, not taking into account the genus Cryptosporidium.…”

Automated Diagnosis of Canine Gastrointestinal Parasites Using Image Analysis

“…In this study we used the technique known as TF-GII/Dog [3] and the Prototic Coproparasitological Test for Dogs (PC-TEST DOG) described below.…”

“…Step 1-Laboratory evaluation of the Parasitological Protocol (PC)-TEST DOG for the DAPI system and creation of an image database composed of parasites and fecal impurities; Step 2-The TF-GII/Dog technique [3] and the PC-TEST DOG protocol were compared against each other, and the same slides were tested in the DAPI system to evaluate the automated detection. The slides were evaluated by two parasitology specialists.…”

“…In regions of developing countries with tropical, subtropical, and equatorial climates, four genera of parasites among metazoan and protozoan stand out as highly prevalent in dog infection: Toxocara spp., Ancylostoma spp., Trichuris spp., and Giardia spp. [2][3][4].…”