Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis

Schoenardie, Elizandra Roselaine; Scaini, Carlos James; Ávila, Luciana Farias da Costa de; Sperotto, Rita Leal; Borsuk, Sibele; Felicetti, Cristine Dias Pires; Pepe, Michele Soares; Berne, Maria Elisabeth Aires

doi:10.1590/s1984-29612014060

Cited by 6 publications

(8 citation statements)

References 20 publications

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“…In mice infected with a single dose or with multiple infections of T. canis eggs, a high AI (about 50%) was observed 40 to 60 DPI SCHOENARDIE et al, 2014). In our study, however, high avidity IgG was detected early after infection (mean AI = 65.1%) and gradually increased for at least 2 months (mean AI = 97.4%), resulting in a strong association between the Avidity and Reactivity Indices at 60 DPI.…”

Section: Discussioncontrasting

confidence: 68%

“…In our study, we evaluated a small sample size to minimize the number of evaluated animals. In terms of challenge, the infection of the rabbits was limited to a single dose of 1000 eggs, which has been used in other studies with murine models (XI & JIN, 1998;CHO et al, 2007;SCHOENARDIE et al, 2014). Thus, future studies are needed, designed to evaluate different subclasses of immunoglobulins and IgG avidity in rabbits subjected to different doses of infection in a long term study, and a larger sample size, to precisely explain the wide individual variations in immune response and to demonstrate the possibility of using rabbits as an animal model for toxocariasis.…”

Section: Discussionmentioning

confidence: 99%

“…The literature on the avidity of anti-Toxocara antibodies in hosts is scanty and limited to mice SCHOENARDIE et al, 2014). Therefore, studying the avidity of anti-Toxocara spp.…”

Section: Introductionmentioning

confidence: 99%

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Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

Bin

Santarém

Laposy

et al. 2015

Rev. Bras. Parasitol. Vet.

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An evaluation was made of the kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with embryonated Toxocara canis eggs. Seventeen four month old New Zealand White rabbits were distributed into two groups. In the experimental group, twelve rabbits were infected orally with 1,000 embryonated T. canis eggs. A second group (n = 5), uninfected, was used as a control. Serum samples were collected for analysis on days 7, 14, 21, 28 and 60 post-infection (DPI). An indirect ELISA test was performed to evaluate the reactivity index (RI) of IgG anti-T. canis antibodies and to calculate the avidity index (AI). The animals showed seroconversion from the 14 th DPI, with high AI (over 50%) except for one animal, which presented an intermediate AI. At 60 DPI, all the animals were seropositive and maintained a high AI. The data indicated that specific IgG antibodies formed early (14 DPI) in rabbits infected with T. canis, with a high avidity index that persisted throughout the course of the infection.

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Section: Discussioncontrasting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

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Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

Bin

Santarém

Laposy

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Depending on the affected organ and the clinical symptoms, toxocariasis can be classified as visceral larva migrans (VLM) or visceral toxocariasis (VT) and ocular larva migrans (OLM) or ocular toxocariasis (OT), covert or common toxocariasis (CT) and neurotoxocariasis (NT) [ 7 ]. Several studies have characterized human toxocariasis as a chronic infection that can persist for several years [ 8 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

New insights into the immunopathology of early Toxocara canis infection in mice

et al. 2015

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BackgroundNematodes of the genus Toxocara are cosmopolitan roundworms frequently found in dogs and cats. Toxocara spp. can accidentally infect humans and cause a zoonosis called human toxocariasis, which is characterized by visceral, ocular or cerebral migration of larval stages of the parasite, without completing its life cycle. In general, chronic nematode infections induce a polarized TH2 immune response. However, during the initial phase of infection, a strong pro-inflammatory response is part of the immunological profile and might determine the outcome and/or pathology of the infection.MethodsParasitological aspects and histopathology during larval migration were evaluated after early T. canis experimental infection of BALB/c mice, which were inoculated via the intra-gastric route with a single dose of 1000 fully embryonated eggs. Innate immune responses and systemic cytokine patterns (TH1, TH2, TH17 and regulatory cytokines) were determined at different times after experimental challenge by sandwich ELISA.ResultsWe found that experimental infection with T. canis induced a mix of innate inflammatory/TH17/TH2 responses during early infection, with a predominance of the latter. The TH2 response was evidenced by significant increases in cytokines such as IL-4, IL-5, IL-13 and IL-33, in addition to increasing levels of IL-6 and IL-17. No significant increases were observed for IL-10, TNF-α or IFN-γ levels. In parallel, parasitological analysis clearly revealed the pattern of larval migration through the mouse organs, starting from the liver in the first 24 h of infection, reaching the peak in the lungs on the 3rd day of infection and finally being found numerously in the brain after 5 days of infection. Peripheral leukocytosis, characterized by early neutrophilia and subsequent eosinophilia, was remarkable during early infection. The tissue damage induced by larvae was evidenced by histopathological analysis of the organs at different time points of infection. In all of the affected organs, larval migration induced intense inflammatory infiltrate and hemorrhage.ConclusionIn conclusion, these new insights into early T. canis infection in mice presented here enabled a better understanding of the immunopathological events that might also occur during human toxocariasis, thus contributing to future strategies of diagnosis and control.

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“…To further examine the difference in IgG affinity between serum samples from stage IV and early-stage breast cancer, all serum samples selected were analyzed using a urea degradation combining ELISA. This assay is a common method to detect the affinity of an antibody (23,24) and also enables the examination of a larger sample size. The results showed no differences between stage IV and early-stage breast cancer in the affinity of anti-MUC1 IgG (t-test, P=0.873).…”

Section: Discussionmentioning

confidence: 99%

Binding of circulating anti-MUC1 antibody and serum MUC1 antigen in stage IV breast cancer

Tang

Cui

Han

et al. 2017

Molecular Medicine Reports

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The present study aimed to investigate the binding of circulating mucin 1 (MUC1) antibody with serum MUC1 antigen in stage IV breast cancer. Serum samples of 61 patients with stage IV breast cancer and 64 patients with early-stage breast cancer were collected. The anti‑MUC1 antibody (IgG) and MUC1 antigen (cancer antigen 15‑3; Ca15‑3) were detected using an indirect enzyme-linked immunosorbent assay (I‑ELISA) and ELISA, respectively. The MUC1 IgG affinity was detected using a urea degradation combining ELISA. Western blot analysis and an inhibition test were performed for verification of the binding of anti‑MUC1 IgG with MUC1 antigen, and their correlation was analyzed. The results showed that there was a negative correlation between anti‑MUC1 IgG and CA15‑3 antigen in stage IV breast cancer when positive CA15‑3 antigen and/or anti‑MUC1 IgG were selected (r=‑0.417; P=0.0044). The positive anti‑MUC1 IgG with positive Ca15‑3 antigen was more common in stage IV breast cancer, compared with early‑stage breast cancer (χ2=4.629; P=0.031), however, Ca15‑3 antigen positivity was higher in stage IV breast cancer, compared with early‑stage breast cancer (χ2=10.58; P=0.001). Anti‑MUC1 IgG was able to bind to the MUC1 antigen in stage IV breast cancer. No differences in the 8R-MUCPT inhibition ratio were found between the two groups (P=0.778), and there were no differences in the affinity of anti‑MUC1 IgG (P=0.873). In stage IV breast cancer, circulating anti‑MUC1 antibody was found to bind serum MUC1 antigen, although their compatibility was low. No significant difference was found in the affinity of the anti‑MUC1 antibody between stage IV breast cancer and early‑stage breast cancer.

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Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis

Cited by 6 publications

References 20 publications

Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

New insights into the immunopathology of early Toxocara canis infection in mice

Binding of circulating anti-MUC1 antibody and serum MUC1 antigen in stage IV breast cancer

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