Molecular and morphological characterization of Contracaecum pelagicum (Nematoda) parasitizing Spheniscus magellanicus (Chordata) from Brazilian waters

Abstract

Three new sequences of Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2) from C. pelagicum parasite of Spheniscus magellanicus, the Magelanicus penguin, were determined from Brazilian waters. The sequences presented 99 and 98% of similarity with C. pelagicum sequences from Argentina, deposited on GenBank for the same genetic region and with a strong statistical support inferred from the phylogenetic tree. The morphological and ultrastructural studies that were carried out confirmed the genetic analysis.Keywords: Anisakidae, penguin, Rio de Janeiro. ResumoForam determinadas três novas sequências da região do Citocromo c-oxidase da subunidade II do DNA mitocondrial (cox-2 mtDNA) de Contracaecum pelagicum, parasito de Spheniscus magellanicus, pinguim Magalhães, de águas brasileiras. As sequências apresentaram 99 e 98% de similaridade com sequências de C. pelagicum da Argentina depositadas no GenBank da mesma região genética com forte suporte estatístico inferido pela arvore filogenética. Estudos morfológicos e ultraestruturais realizados confirmaram a identidade genética.

Occurrence of gastrointestinal parasites in Spheniscus magellanicus (Foster, 1781) located in Pontal do Sul, PR, Brazil

“…This fact, combined to the restrict habitat of the definitive hosts might be the main aggravating factors of the low prevalence of T. lutzi also observed in other studies (González-Acuña et al, 2009;Mäder et al, 2010;Rezende et al, 2013). Diaz et al (2010) working in the Valdez Peninsula, Argentina, found T. lutzi as the most prevalent helminth, probably as a result of the geographic distribution of its intermediary hosts, which are normally present in this region (Borges et al, 2014). In our study, we were surprise by the enormous numbers of this parasite infecting most of the animals.…”

The occurrence of Contracaecum sp. larvae (Nematoda: Anisakidae) in four teleostean species from Lake Nasser, Egypt: morphological and molecular studies

“…from various host groups attracted attention of scientists in different geographical parts of the world using both light and scanning electron microscopy for morphological examination, as well as the evaluation of genetic markers as the ribosomal internal transcribed spacers (ITS-1 and ITS-2) for the molecular characterization (Nadler, D'Amelio, Dailey, Paggi, Siu, et al, 2005;D'Amelio, Barros, Ingrosso, Fauquier, Russo, et al, 2007;Shamsi, Gasser, Beveridge, & Shabani, 2008;Shamsi, Norman, Gasser, & Beveridge, 2009;Shamsi, Gasser, & Beveridge, 2011;Garbin, Mattiucci, Paoletti, González-Acuña, & Nascetti, 2011;Garbin, Mattiucci, Paoletti, Diaz, Nascetti, et al, 2013;Jabbar, Fong, Kok, Lopata, Gasser, et al, 2013;Borges, Santos, Brandã, Santos, Miranda, et al, 2014).…”

A new species of Contracaecum (Nematoda: Anisakidae) found parasitizing Nannopterum brasilianus (Suliformes: Phalacrocoracidae) and Hoplias argentinensis (Characiformes: Erythrinidae) in South America: morphological and molecular characterization of larval and adult stages

“…The direct relationship between adults and larval stages of C. jorgei n. sp. and the interspecific variation found in this study show that mt cox -2 can be a valuable tool for species identification within the genus (Jex et al ., 2008; Borges et al ., 2014). Therefore, due to the biodiversity of Anisakidae nematodes, the analysis of molecular genetic markers has been shown to be an efficient tool for specific diagnosis and unequivocal identification of anisakid nematodes with zoonotic potential (Kanarek & Bohdanowicz, 2009).…”