Optimization of protease production by the fungusMonacrosporium thaumasium and its action againstAngiostrongylus vasorum larvae

Soares, Filippe Elias de Freitas; Braga, Fábio Ribeiro; Araújo, Jackson Víctor de; Lima, Walter dos Santos; Mozzer, Lanuze Rose; Queiróz, José Humberto de

doi:10.1590/s1984-29612013000200048

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…In this study, we used 2% chitin agar culture medium as a source of chitin for the growth of the VC4 isolate and, subsequently, its possible enzymatic action. Our results agree with other studies that suggest the production of certain enzymes by nematophagous fungi, depending on the type of substrate in which they are cultivated (Soares et al 2013).…”

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confidence: 93%

Colonization and destruction of ants of the genus Camponotus sp. (Hymenoptera: Formicidae) in vitro by the fungus Pochonia chlamydosporia in the southeast region of Brazil

et al. 2018

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The objective of this study was to evaluate, in vitro, the colonization and destruction of ants of the genus sp. by the ovicidal fungus (VC4 isolate), in the southeast region of Brazil. The insects used in the experiment were worker ants of the genus sp., collected periodically in the environment and immediately transported to the laboratory in test tubes. Then, VC4 growth was promoted in 2% chitin agar medium (2% WQ) to obtain a fungal solution containing conidia and/or chlamydospores. Two experimental groups were formed. Treated group consisted of Petri dishes containing 2% agar-water culture medium (2% WA) with nine live insects and 20 µL of fungal solution at the concentration of 15,000 conidia/chlamydospores. Control group consisted of Petri dishes containing 2% WA culture medium and nine live insects. The dishes in the treated and control groups were incubated in BOD at 25 ± 1 °C and 80 ± 10% relative humidity for 4 days. After 4 days, it was observed that the VC4 had grown, colonized, and caused the destruction of the ants. The fungus was efficient at colonizing and destroying the urban ants collected on an experimental basis. Thus, it could open up new ways to reduce the use of chemical compounds in the future, decreasing health and environmental problems.

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confidence: 93%

Colonization and destruction of ants of the genus Camponotus sp. (Hymenoptera: Formicidae) in vitro by the fungus Pochonia chlamydosporia in the southeast region of Brazil

et al. 2018

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“…The inoculum has grown in shaken flasks at 120 × g. After 6 days, proteases were obtained in its crude form, by filtration using Whatman no.1 filter paper, followed by centrifugation for 5 min at 10 × g and 4°C. The supernatant (crude proteases) was used in the subsequent assays [8]. …”

Section: Resultsmentioning

confidence: 99%

“…An in vitro assay was conducted to evaluate the nematicidal action of the proteases of A. sinensis (SF53) produced in liquid medium on A. vasorum L 1 following the methodology of Soares and colleagues [8]. Two groups were formed in sterile tubes, a treated group containing the crude proteases and a control group (without enzyme), which were then incubated at 26°C in the dark for 24 h. A total of 100 A. vasorum L 1 were poured into sterile tubes containing the crude enzyme.…”

Section: Resultsmentioning

confidence: 99%

Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae

et al. 2014

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BackgroundThe predatory nematophagous fungus Arthrobotrys sinensis (SF53) produces three proteases with nematicidal activity when grown on solid media culture. However, the proteolytic profile produced by this fungus, when grown in liquid culture medium remains unknown.FindingsThus, the objective of this work was to evaluate the production of proteases from nematophagous fungus Arthrobotrys sinensis in liquid medium and its nematicidal activity on first stage larvae of A. vasorum. Proteases were obtained in its crude form, using Whatman no.1 filter paper, followed by centrifugation for 5 min at 10 × g and 4°C. A zymogram was performed with co-polymerized casein in an acrylamide gel as substrate. An in vitro assay to evaluate the nematicidal action of the proteases of A. sinensis (SF53) produced in liquid medium on A. vasorum L1 was conducted. By the analysis of the zymogram, it was observed a single halo at the beginning of digestion of the gel, suggesting that the three proteases of SF53 are produced in an enzymatic complex of large molecular weight. Regarding nematicidal activity, within 24 hours, the proteases produced in liquid medium of A. sinensis (SF53) showed a percentage reduction of 64% on the number of L1 of A. vasorum.ConclusionIn the present work, it is suggested that the three proteases of SF53 are produced in an enzymatic complex and was also demonstrated that these enzymes were effective in destroying A. vasorum L1.

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“…Accordingly, the species M. thaumasium (NF34) produces chitinases that act on experimental model of freeliving nematodes, which represents a new step in understanding the interaction of extracellular enzymes with the cuticle of the nematode (Soares et al, 2014). The species M. sinense produced extracellular proteases that are important virulence factors on nematodes (Soares et al, 2013b).…”

Section: Discussionmentioning

confidence: 99%

Statistical screening for the chitinase production by nematophagous fungi from Monacrosporium genu

Filippe¹,

Jose²,

Jackson³

et al. 2015

Afr. J. Microbiol. Res.

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Several chitinases with nematicidal action of these fungi have been identified in recent years, however, little is known about which nutritional factors from the culture medium are significant for its mass production. The aim of this study was to perform the statistical screening of the components of the culture medium for chitinase production by nematophagous fungi, Monacrosporium sinense (SF53) and Monacrosporium thaumasium (NF34) aiming to identify which of these components were significant and to evaluate the influence of pH on chitinase activity of these fungi. The effect of five variables (chitin, colloidal chitin, glucose, sodium nitrate and moisture) on chitinase production was analyzed using Plackett-Burman statistical design. Chitinase activity was determined at different pH (pH 3.5, 4.5 and 5.5) and Tris-HCl 50 mM (pH 7.0 and 8.0). In relation to the isolate NF34, at the levels evaluated, two variables were significant (p <0.05) for chitinase production: chitin and NaNO 3. On the other hand, for SF53, at the levels evaluated, only glucose was significant (p <0.05). Regarding the influence of pH on enzyme activity for M. thaumasium the pH value with the highest activity obtained was 5.5. However, for M. sinense, the highest activity was observed at pH 8. These data demonstrate that different species of nematophagous fungi may exhibit better enzymatic activities and consequently, better predatory activities at different environmental conditions.

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Optimization of protease production by the fungusMonacrosporium thaumasium and its action againstAngiostrongylus vasorum larvae

Cited by 6 publications

References 12 publications

Colonization and destruction of ants of the genus Camponotus sp. (Hymenoptera: Formicidae) in vitro by the fungus Pochonia chlamydosporia in the southeast region of Brazil

Colonization and destruction of ants of the genus Camponotus sp. (Hymenoptera: Formicidae) in vitro by the fungus Pochonia chlamydosporia in the southeast region of Brazil

Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae

Statistical screening for the chitinase production by nematophagous fungi from Monacrosporium genu

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