In vitro biological control of infective larvae of Ancylostoma ceylanicum

Fernandes, Fernanda Mara; Araújo, Jackson Víctor de; Braga, Fábio Ribeiro; Gazzinelli-Guimarães, Pedro Henrique; Araújo, Juliana Milani; Ferreira, Sebastião Rodrigo; Carvalho, Rogério Oliva; Mello, Ingrid Ney Kramer de; Fujiwara, Ricardo Toshio

doi:10.1590/s1984-29612012000300018

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…While some filamentous fungi are able for breaking the eggshells from certain helminths [14, 15], others are able for capturing and destroying infective larvae [16, 17]. These are organisms innocuous for animals and plants which spread as spores, and large amounts for reaching successful results in the control of parasites look necessary [18].…”

Section: Introductionmentioning

confidence: 99%

Mixed Production of Filamentous Fungal Spores for Preventing Soil-Transmitted Helminth Zoonoses: A Preliminary Analysis

Arias

Cazapal-Monteiro

Miguélez

et al. 2013

BioMed Research International

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Helminth zoonoses are parasitic infections shared by humans and animals, being the soil-transmitted helminths (STHs) mainly caused by roundworms (ascarids) and hookworms. This study was aimed to assess the individual and/or mixed production of two helminth-antagonistic fungi, one ovicide (Mucor circinelloides) and other predator (Duddingtonia flagrans). Fungi were grown both in Petri plates and in a submerged culture (composed by water, NaCl, Na2HPO4 · 12 H2O, and wheat (Triticum aestivum)). A Fasciola hepatica recombinant protein (FhrAPS) was incorporated to the cultures to improve fungal production. All the cultured plates showed fungal growth, without difference in the development of the fungi when grown alone or mixed. High counts of Mucor spores were produced in liquid media cultures, and no significant differences were achieved regarding single or mixed cultures, or the incorporation of the FhrAPS. A significantly higher production of Duddingtonia spores after the incorporation of the FhrAPS was observed. When analyzing the parasiticide efficacy of the fungal mixture, viability of T. canis eggs reduced to 51%, and the numbers of third stage cyathostomin larvae reduced to 4%. It is concluded, the capability of a fungal mixture containing an ovicide (Mucor) and a predator species (Duddingtonia) for growing together in a submerged medium containing the FhrAPS offers a very interesting tool for preventing STHs.

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Section: Introductionmentioning

confidence: 99%

Mixed Production of Filamentous Fungal Spores for Preventing Soil-Transmitted Helminth Zoonoses: A Preliminary Analysis

Arias

Cazapal-Monteiro

Miguélez

et al. 2013

BioMed Research International

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Nematophagous fungi for biological control of gastrointestinal nematodes in domestic animals

Braga

Araújo

2013

Appl Microbiol Biotechnol

145

101

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Several studies have been conducted using fungi in the biological control of domestic animals and humans. In this respect, a large amount of research has been undertaken to understand the particularities of each fungus used. These fungi have been demonstrated to act on all classes of helminthes. Therefore, they should not only be called nematophagous but also helmintophagous. Evidence of enzymatic action has also revealed their mechanism of action, as well as potential metabolites that could be synthesized as bioactive molecules. Cultural barriers to the use of fungi should be broken down, since the impact on the environment is minimal. In this context, much is already known about the mechanism of interaction of these organisms with their 'targets'. Recent research has pointed to the search for substances derived from nematophagous fungi that have demonstrated their ovicidal and/or larvicidal activity, thus being a global premise to be studied further. Crude extracts derived from nematophagous fungi of predator and ovicidal groups reduce the amount of larvae of gastrointestinal nematodes and prevent the hatching of their eggs, since they have been demonstrated to act with extracellular proteases and other enzymes. Furthermore, the activity of these enzymes has begun to be explored regarding their possible interaction with the exoskeleton of arthropods, which could emerge as an alternative method of tick control. Finally, it should be clear that nematophagous fungi in general are 'old friends' that are ready to the 'fight with our old enemies', the gastrointestinal helminth parasites harmful to human and animal health.

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