Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture

Abstract

<jats:p>The protective efficacy of an inactivated vaccine from Anaplasma marginale that was cultured in tick cells (IDE8) for use against bovine anaplasmosis was evaluated. Five calves (Group 1) were inoculated subcutaneously, at 21-day intervals, with three doses of vaccine containing 3 × 10(9) A. marginale initial bodies. Five control calves received saline solution alone (Group 2). Thirty-two days after the final inoculation, all the calves were challenged with approximately 3 × 10(5) erythrocytes infected with A. marginale high-virulence isolate (UFMG2). The Group 1 calves seroconverted 14 days after the second dose of vaccine. After the challenge, all the animals showed patent rickettsemia. There was no significant difference (p &gt; 0.05) between the Group 1 and 2 calves during the incubation period, patency period or convalescence period. All the animals required treatment to prevent death. The results suggest that the inactivated vaccine from A. marginale produced in IDE8 induced seroconversion in calves, but was not effective for preventing anaplasmosis induced by the UFMG2 isolate under the conditions of this experiment.</jats:p>

Knockdown of the Rhipicephalus microplus Cytochrome c Oxidase Subunit III Gene Is Associated with a Failure of Anaplasma marginale Transmission

“…In Latin America, it is estimated that bovine anaplasmosis and babesiosis cause annual economic losses exceeding US$ 800 million [2]. In endemic regions, anaplasmosis control strategies include the use of a live-attenuated vaccine, a killed vaccine, antibiotic prophylaxis and/or tick control measures [3], [4]. Vaccines are the most effective method for controlling disease and induce protective immunity that prevents acute bacteremia.…”

“…Vaccines are the most effective method for controlling disease and induce protective immunity that prevents acute bacteremia. However, vaccines do not prevent A. marginale infection, and infected animals can serve as reservoirs for tick transmission [1], [4].…”

Immune response of calves inoculated with proteins ofAnaplasma marginale bound to an immunostimulant complex

“…Protection against bovine anaplasmosis has been the target of several studies (LOPEZ et al, 2007;PALMER et al, 2011;DARK et al, 2011;LASMAR et al, 2012). Although previous studies have shown that the A. marginale subsp.…”

Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules

“…The production of crude antigen was performed by cultivating A. marginale (UFMG2) in IDE8 cells (BASTOS et al, 2009). Initial bodies produced in vitro were purified (LASMAR et al, 2012); thereafter, the sample was sonicated (Ultrasonic Homogenizer 4710, Cole Parmer  ), and an aliquot was used for protein quantification (BRADFORD, 1976). The remaining sample was stored at -20 °C.…”

“…Establishment of A. marginale growth in embryonic cells from the Ixodes scapularis tick (IDE8) was considered a promising alternative in the production of inactivated vaccine. However, this vaccine failed to fully protect adult cattle from clinical disease (GARCIA-GARCIA et al, 2004;LASMAR et al, 2012). One possible reason for the failure could be related to the absence of the Major Surface Protein 1a (MSP1a) expression in this system, due to down-regulation of the msp1a gene in A. marginale derived from cultured tick cells (GARCIA-GARCIA et al, 2004).…”