Expressão e atividade enzimática de glutationa s-transferase em tecidos de fêmeas de Boophilus microplus

Freitas, Daniela Reis Joaquim de; Vaz, Itabajara da Silva; Masuda, Aoi

doi:10.1590/s1984-29612008000200007

Cited by 12 publications

(5 citation statements)

References 38 publications

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“…We observed an association of these factors with the increased GST activity. In ticks, endogenous oxidative damage was generated by the products of the metabolism obtained from food intake, and associated with increased GST activity (Freitas et al 2008). We also observed that the depth of the temporary ponds was an important factor influencing the levels of TBARS and GST activity.…”

Section: Discussionmentioning

confidence: 64%

“…We suggest that GST may have an important function during the period of sexual maturation and pre-posture, acting possibly in the process of biotransformation of sexual hormones secreted in this stage. Freitas et al (2008) and Pinheiro and Oliveira (2016) also suggest GST at this stage of the animals' life cycle; however, with ticks and crayfish, respectively. GSTs are present in different organisms and are important for the intracellular biotransformation of endo and xenobiotics (Papadopoulos et al 2004).…”

Section: Discussionmentioning

confidence: 97%

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Biomarkers of oxidative stress in the post-embryonic characterization of the neotropical annual killifish

et al. 2021

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Annual killifish are among the most remarkable extremophile species with the shortest vertebrate life span. Few studies have reported on the oxidative balance throughout their life cycle and its association to the natural aging process of these neotropical animals in a natural environment. We standardized and analyzed physiological markers related to the redox balance of the annual killifish (Cynopoecilus fulgens) throughout the post-embryonic life cycle (enzyme activity of Superoxide Dismutase, Catalase, Glutathione Peroxidase, and Glutathione S-transferase, as well as the determination of the levels of Lipoperoxidation, Carbonylated Proteins, and Total Proteins). We tested the influence of environmental variables on these biomarkers. Individuals were collected, including juveniles, adults, and seniles, in three sampling units around the Parque Nacional da Lagoa do Peixe, located in the Coastal Plain of Rio Grande do Sul. We observed that males and females used different physiological strategies of their redox balance during their life cycle, and their oxidative balance was influenced by their reproductive period and environmental variables (water temperature, abundance of predators, abundance of another sympatric annual killifish species, and abundance of C. fulgens). The population of each temporary pond presented different physiological responses to the adaptation of their life cycle, and there was an influence of environmental component as a modulator of this cycle. Our study offers reference values that will be useful for comparison in future research with short-lived organisms.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 97%

Biomarkers of oxidative stress in the post-embryonic characterization of the neotropical annual killifish

et al. 2021

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“…In humans there is an increase in infectious diseases such as conjunctivitis [ 60 ]. Glutathione S transferase Acts as an antioxidant in the eye system [ 61 , 62 ]. Polymeric immunoglobulin receptor This glycoprotein has already been described in tear samples of humans.…”

Section: Discussionmentioning

confidence: 99%

Protein expression of the tear film of domestic cats before and after inoculation with Toxoplasma gondii

et al. 2021

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Background Tear film (TF) helps maintain and protect ocular function against damage to the ocular surface. Proteins are one of its main constituents, whose expression pattern can be used as a biomarker of ocular changes and systemic diseases. The aim of this study was to evaluate the expression of proteins in the TF of domestic cats before and after infection with Toxoplasma gondii, in the phases of acute infection and chronicity. Twelve healthy cats received orally homogenized brain matter obtained from mice inoculated with T. gondii oocysts, strain ME49. Cat feces were collected daily from the third day after infection to assess the release of oocysts. TF samples were obtained from cats, by Schirmer’s Tear Test 1, on day 0 (before infection), day 5 after infection (acute phase of infection, with maximum peak release of oocysts in feces) and on day 21 after infection (start of chronic phase, 7 days after total absence of oocyst release in feces). Tear samples were also submitted to proteomic analysis in a Q-Tof-Premier mass spectrometer. Results A total of 37 proteins with scores equal to or greater than 100 were identified on D0, followed by 36 on D5 and 42 on D21. Of these, 27 were common to D0 and D5, 33 to D0 and D21, 27 to D5 and D21, and 26 were common to the three groups, totaling 54 proteins. The most abundant proteins were lipocalin allergen Fel d, serum albumin, aldehyde dehydrogenase, lactoperoxidase and lactotransferrin. There was no significant difference in the abundance of proteins found on D0 and D5, but there was a statistical difference between D0 and D21 for ACT1_AEDAE, CERU_HUMAN and GELS_HUMAN. Regarding D5 and D21, there were significant differences for KV1_CANLF, LAC_PIG, TRFL_PIG, ACT1_AEDAE, CERU_HUMAN, GELS_HUMAN and OVOS2_HUMAN. Conclusions The main proteins identified in the TF of domestic cats are similar to those found in humans and other animal species. Most are part of the ocular surface defense system against injuries. The most expressed proteins in animals in the chronic phase of T. gondii infection are associated with the immune response to the parasite.

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“…Glutathione S-transferases (GSTs) denotes a superfamily of catalytic proteins that are present in almost all living beings, being distributed in a great diversity of aerobic organisms, ranging from bacteria to human [10][11]. These enzymes are involved in several biological processes [13].…”

Section: Highlightsmentioning

confidence: 99%

Possible Metsulfuron Herbicide Detoxification by a Oryza sativa L. Glutathione S-transferase Enzyme.

Amador

Silva

Nadvorny

et al. 2020

Braz. arch. biol. technol.

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Rice (Oryza sativa L.) is one of the most important crops in the world, and it is considered the primary source of nutritional layout in developing countries in Asia. The glutathione S-transferases (GSTs) superfamily confers to rice protection against biotic and abiotic stress, and herbicide resistance. However, the three-dimensional structure of a GST Tau class, is unsolved. The objectives of this work were to develop a reliable comparative model for the s-transferase glutathione class Tau 4 from rice, and simulate docking interactions, against herbicides bentazon and metsulfuron. Results showed that the predicted model is reliable and has structural quality. Ramachandran plot set 91.9% of the residues in the most favored regions. All complexes showed negative binding energies values; and metsulfuron docked to the glutathione tripeptide, and it represents a possible insilico evidence of glutathione conjugation with this herbicide.

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Expressão e atividade enzimática de glutationa s-transferase em tecidos de fêmeas de Boophilus microplus

Cited by 12 publications

References 38 publications

Biomarkers of oxidative stress in the post-embryonic characterization of the neotropical annual killifish

Biomarkers of oxidative stress in the post-embryonic characterization of the neotropical annual killifish

Protein expression of the tear film of domestic cats before and after inoculation with Toxoplasma gondii

Possible Metsulfuron Herbicide Detoxification by a Oryza sativa L. Glutathione S-transferase Enzyme.

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