Influência do comportamento gregário nos processos de muda de Rhipicephalus sanguineus (Latreille, 1806) (acari: ixodidae), em condições laboratoriais

Abstract

RESUMOObjetivou-se com o experimento verificar a influência de diferentes agregações sobre os parâmetros relacionados ao processo de ecdise ninfal e de adulto de Rhipicephalus sanguineus (Latreille, 1806 (Latreille, 1806). Tick-naïve domestic rabbits (Oryctolagus cuniculus) were used as experimental hosts for the parasitic phase. A total of 10 rabbits were used on the experiment. All tick free-living phases were kept in incubator at 27±1 o C, 80±5% relative humidity and scotophase. The periods of pre-molt, molt, percentage of ecdisis and molt rhythm of larvae and nymphs were studied. A total of 1560 engorged larvae and 1560 nymphs of R. sanguineus were divided in six groups of aggregations: 1, 5, 10, 20, 40 and 80 ticks with ten repetitions each one. The ecdises of nymphs and adults were daily observed. The percentage of ecdysis was the only parameter with no aggregations effects. All others parameters were statistically different (p<0,05). The results suggested that highest aggregations accelerate the molting process.

Prognosing the sex of adults of Amblyomma auricularium through evaluating the biological parameters of engorged nymphs

“…In this study, we used engorged nymphs of A. auricularium that were collected between the 7 th and 12 th day after infestation of four tick-naive rabbits experimentally infested. Some authors who used the same technique recovered engorged nymphs of H. leporispalustris between the sixth and ninth days after infestation (FREITAS et al, 2000), while others recovered nymphs of R. sanguineus on the third day after infestation (BARBIERI et al, 2003), all with weight lower than what was observed in A. auricularium this study. Ciência Rural, v.43, n.4, abr, 2013.…”

“…In relation to A. cajennense (OLIVIERI et al, 1986, BARBIERI et al, 2001 and R. sanguineus (BARBIERI et al, 2003), overlapping weight classes between nymphs that originated males and females were also observed. For these species, this parameter was considered impractical for predicting the sex of the adult form.…”

“…To establish colonies of arthropods in a laboratory, adequate equipment, personnel qualifi ed to deal with these arthropods and, especially, biological knowledge of the species that will be kept in the laboratory is required (CANÇADO et al, 2007). Through knowledge of species biology, colony maintenance and experiments that are designed using ixodid ticks may be improved.…”

“…The biological parameters of nymphs have previously been evaluated with the aim of determining the sex of adults of some species of Neotropical ticks. The weight of engorged nymphs, engorgement period and premolt period have been the most commonly used parameters, as can be seen in relation to Amblyomma cajennense (FABRICIUS, 1787) (OLIVIERI et al, 1986BARBIERI et al, 2001;PINTER et al, 2002), Amblyomma tigrinum (Kock, 1844) (LABRUNA et al, 2002, Amblyomma triste (Kock, 1844) (LABRUNA et al, 2003, Haemaphysalis leporispalustris (Packard, 1869) (FREITAS et al, 2000 and Rhipicephalus sanguineus (Latreile, 1806) (BARBIERI et al, 2003. It is important to prognose the sex of adult specimens while they are still at the nymphal stage, in order to optimize experiments under laboratory conditions, especially those that require previous determination.…”

Biology and ecology of the brown dog tick, Rhipicephalus sanguineus

“…sanguineus ticks. What is known is that aggregation accelerates the moulting process of nymphs [29]. Interestingly, the presence of newly moulted nymphs appears to act as a mechanical stimulus for the ecdysis of other nymphs (see additional file 3) (unpublished observations).…”